In order to investigate the expression levels of Pinl mRNA and protein in cervical cancer and its association with Ki67 and their clinical significance, amplification of Pinl gene was examined by RT-PCR, and the expre...In order to investigate the expression levels of Pinl mRNA and protein in cervical cancer and its association with Ki67 and their clinical significance, amplification of Pinl gene was examined by RT-PCR, and the expression of both Pinl and Ki67 protein was detected by immunohistochemistry in cervical cancer tissues. It was shown that the expression levels of Pinl were higher in cervical cancer than in normal cervical tissues （P〈0.05）. The expression of Pinl protein was increased progressively along with the disease process from normal cervix to CIN and to cervical cancer （P〈0.05）. No significant difference in the Pinl expression was found between disease stages （FIGO）, pathological grades or pelvic lymph node metastasis status （P〉0. 05）. The expression of Pin1 was significantly higher in adenocarcinoma than in squamous carcinoma of the uterine cervix （P〈0.05）. In cervical cancer, the overexpression of Pinl was positively correlated with that of Ki67 （P〈 0. 05）. These results suggested that the overexpression of Pinl was closely related with cancer cell proliferation or progression of cervical cancer and contributed to oncogenesis. Pinl may serve as a potential marker for cervical cancer diagnosis.展开更多
Objective: To investigate the expressions and correlations of Pin1, β-catenin and cyclin D1 in elderly lung car-cinomas. Methods: The expressions of Pin1, β-catenin and cyclin D1 were examined in the specimens of 92...Objective: To investigate the expressions and correlations of Pin1, β-catenin and cyclin D1 in elderly lung car-cinomas. Methods: The expressions of Pin1, β-catenin and cyclin D1 were examined in the specimens of 92 elderly lung carcinomas and 10 normal lung tissues by immunohistochemistry and explored the relationship between the expression levels and clinicopathological factors. Results: (1) The overexpression of Pin1 and cyclin D1 in lung carcinomas was 46 (50%) cases and 60 (65.22%) cases respectively and 56 (60.82%) cases showed positive immunoreactivity for β-catenin in the nuclear and (or) cytoplasmic fraction in tumor tissues. In normal tissue, the expressions of Pin1 and cyclin D1 were negative, the expression of β-catenin was lied in cell membrane. (2) In lung carcinomas the expressions of Pin1, β-catenin and cyclin D1 correlated with tumor differentiation (P < 0.05). The positive expression rate and intensity of Pin1 correlated with tumor stage (P = 0.032) and lymph node positive disease (P = 0.041). The expression of β-catenin correlated with lymph node positive disease (P = 0.012). (3) High expression levels of Pin1 correlated with aberrant β-catenin expression (P = 0.000) but did not show a correlation with cyclin D1 (P = 0.157). Conclusion: In elderly lung carcinomas, the positive expression of Pin1 causes abnormal accumulation of β-catenin and actives its target gene, however, this target gene was not cyclin D1. The detection of Pin1 expression had some clinical significance in estimating prognosis of elderly patient with lung carcinomas.展开更多
基金This project was supported by grants from National Key Basic Research （973） Fund （No. 2002CB513107） and National Natural Science Foundation of China （No. 30271358）.
文摘In order to investigate the expression levels of Pinl mRNA and protein in cervical cancer and its association with Ki67 and their clinical significance, amplification of Pinl gene was examined by RT-PCR, and the expression of both Pinl and Ki67 protein was detected by immunohistochemistry in cervical cancer tissues. It was shown that the expression levels of Pinl were higher in cervical cancer than in normal cervical tissues （P〈0.05）. The expression of Pinl protein was increased progressively along with the disease process from normal cervix to CIN and to cervical cancer （P〈0.05）. No significant difference in the Pinl expression was found between disease stages （FIGO）, pathological grades or pelvic lymph node metastasis status （P〉0. 05）. The expression of Pin1 was significantly higher in adenocarcinoma than in squamous carcinoma of the uterine cervix （P〈0.05）. In cervical cancer, the overexpression of Pinl was positively correlated with that of Ki67 （P〈 0. 05）. These results suggested that the overexpression of Pinl was closely related with cancer cell proliferation or progression of cervical cancer and contributed to oncogenesis. Pinl may serve as a potential marker for cervical cancer diagnosis.
文摘Objective: To investigate the expressions and correlations of Pin1, β-catenin and cyclin D1 in elderly lung car-cinomas. Methods: The expressions of Pin1, β-catenin and cyclin D1 were examined in the specimens of 92 elderly lung carcinomas and 10 normal lung tissues by immunohistochemistry and explored the relationship between the expression levels and clinicopathological factors. Results: (1) The overexpression of Pin1 and cyclin D1 in lung carcinomas was 46 (50%) cases and 60 (65.22%) cases respectively and 56 (60.82%) cases showed positive immunoreactivity for β-catenin in the nuclear and (or) cytoplasmic fraction in tumor tissues. In normal tissue, the expressions of Pin1 and cyclin D1 were negative, the expression of β-catenin was lied in cell membrane. (2) In lung carcinomas the expressions of Pin1, β-catenin and cyclin D1 correlated with tumor differentiation (P < 0.05). The positive expression rate and intensity of Pin1 correlated with tumor stage (P = 0.032) and lymph node positive disease (P = 0.041). The expression of β-catenin correlated with lymph node positive disease (P = 0.012). (3) High expression levels of Pin1 correlated with aberrant β-catenin expression (P = 0.000) but did not show a correlation with cyclin D1 (P = 0.157). Conclusion: In elderly lung carcinomas, the positive expression of Pin1 causes abnormal accumulation of β-catenin and actives its target gene, however, this target gene was not cyclin D1. The detection of Pin1 expression had some clinical significance in estimating prognosis of elderly patient with lung carcinomas.
