Recurrent pregnancy loss （RPL） is a condition with complex etiologies, to which both genetic and envi- ronmental factors may contribute. During the last decade, studies indicated that the expression patterns of the ...Recurrent pregnancy loss （RPL） is a condition with complex etiologies, to which both genetic and envi- ronmental factors may contribute. During the last decade, studies indicated that the expression patterns of the pro- kineticin receptor （PKR1 and PKR2） are closely related to early pregnancy. However, there are few studies on the role of PKR1 and PKR2 in RPL. In this study, we purpose to investigate the association between polymorphisms of the prokineticin receptor （PKR1 rs4627609 and PKR2 rs6053283） and RPL on a group of 93 RPL cases and 169 healthy controls. Genotyping of the single nucleotide polymorphisms （SNPs） was performed using a Sequenom MassARRAY iPLEX system. The results revealed a significant association between PKR2 rs6053283 polymorphism and RPL （P=0.003）, whereas no association was observed between PKR1 rs4627609 polymorphism and RPL （P=-0.929） in the Chinese Han population.展开更多
The interferon-inducible double-stranded RNA-dependent protein kinase PKR has been suggested to function as a turnour suppressor gene product.Catalytically inactive mutants of PKR give rise to a tumourigenic phenotype...The interferon-inducible double-stranded RNA-dependent protein kinase PKR has been suggested to function as a turnour suppressor gene product.Catalytically inactive mutants of PKR give rise to a tumourigenic phenotype when overexpressed in NIH-3T3 fibroblasts and this has been attributed to a dominant negative effect on only inhibits the protein kinase activity of wild-type PKR but is also inhibitory towards another dotlblestranded RNA-dependent enzyme,the 40kDa form of 2'5'oligoadenylate synthetase. Inhibition of both wile-type PKR or 2'5' oligoadenylate synthetase is reversed by adding higher concentrations of double-stranded RNA. These results suggest competition between PKR K296R and wild-type PKR or 2'5' oligoadenylate synthetase for limiting amounts of dublestranded RNA. Moreover,the data imply that the tumourigenic effect of this PKR mutant could be due to inhibition of additional pathways requiring low levels of double-strandeed RNA for activation and cannot be unambiguously attributed to inhibition of endogenous PKR itself.展开更多
基金Project supported by the National Natural Science Foundation of China(No.81571503)
文摘Recurrent pregnancy loss （RPL） is a condition with complex etiologies, to which both genetic and envi- ronmental factors may contribute. During the last decade, studies indicated that the expression patterns of the pro- kineticin receptor （PKR1 and PKR2） are closely related to early pregnancy. However, there are few studies on the role of PKR1 and PKR2 in RPL. In this study, we purpose to investigate the association between polymorphisms of the prokineticin receptor （PKR1 rs4627609 and PKR2 rs6053283） and RPL on a group of 93 RPL cases and 169 healthy controls. Genotyping of the single nucleotide polymorphisms （SNPs） was performed using a Sequenom MassARRAY iPLEX system. The results revealed a significant association between PKR2 rs6053283 polymorphism and RPL （P=0.003）, whereas no association was observed between PKR1 rs4627609 polymorphism and RPL （P=-0.929） in the Chinese Han population.
文摘The interferon-inducible double-stranded RNA-dependent protein kinase PKR has been suggested to function as a turnour suppressor gene product.Catalytically inactive mutants of PKR give rise to a tumourigenic phenotype when overexpressed in NIH-3T3 fibroblasts and this has been attributed to a dominant negative effect on only inhibits the protein kinase activity of wild-type PKR but is also inhibitory towards another dotlblestranded RNA-dependent enzyme,the 40kDa form of 2'5'oligoadenylate synthetase. Inhibition of both wile-type PKR or 2'5' oligoadenylate synthetase is reversed by adding higher concentrations of double-stranded RNA. These results suggest competition between PKR K296R and wild-type PKR or 2'5' oligoadenylate synthetase for limiting amounts of dublestranded RNA. Moreover,the data imply that the tumourigenic effect of this PKR mutant could be due to inhibition of additional pathways requiring low levels of double-strandeed RNA for activation and cannot be unambiguously attributed to inhibition of endogenous PKR itself.
