Objective To observe the changes of sciatic nerve conduction velocity （SNCV） and interleukin-1α （IL-1α）, phospholipase A2 （PLA2） and prostaglandin E2 （PGE2） in neucleus pulposus tissue in experimental rabbit...Objective To observe the changes of sciatic nerve conduction velocity （SNCV） and interleukin-1α （IL-1α）, phospholipase A2 （PLA2） and prostaglandin E2 （PGE2） in neucleus pulposus tissue in experimental rabbits with lumbar disc herniation （LDH） ; and discuss the mechanism of LDH treated with bloodletting on Wěizhōng （委中 BL40）. Methods 40 normal named control group, LDH model group, BL40 group New Zealand rabbits were randomized into 4 groups, and comparative point group. The experimental LDH model was prepared by the self-made LDH animal pathological modeling device. Bloodletting on BL40 or on comparative point was applied for 7 days. BL-410 physiological signal recorder was used to record SNCV. Enzyme-linked immunosorbent assay （ELISA） and radioimmunology methods were used to determine the contents of IL-1α and PLA2 in neucleus pulposus tissue and PGE2 in serum. Results In BL40 group, SNCV was significantly increased after treatment comparing with that before treatment （P〈0. 01 ）, and the difference in SNCV value before and after treatment was significantly bigger than that in model group and comparative point group （P〈0.05）. Bloodletting on BL40 reduced the contents of IL-1α and PLA2 in neucleus pulposus tissue and serum PGE2 in LDH rabbits, which indicated significant difference or very significant results in the comparison with model and comparative point groups （P〈0.01 or 0. 05）. Conclusion Bloodletting speeded up rapidly SNCV and reduced the contents of inflammatory factors, i.e. IL-1α, PLA2 and serum PGE2 in LDH rabbits, which indicated that alleviation of local inflammatory reaction is probably one of the mechanisms on LDH treated by bloodletting on BL40.展开更多
目的研究亲磷酸酯酶A1(PA-PLA1)基因在小鼠分泌胰岛素细胞株M IN 6中的表达及其与胰岛素分泌的关系。方法对M IN 6细胞进行不同浓度和不同时间的葡萄糖刺激胰岛素分泌的实验,酶联免疫吸附法测定细胞上清液中胰岛素分泌量,实时荧光定量P...目的研究亲磷酸酯酶A1(PA-PLA1)基因在小鼠分泌胰岛素细胞株M IN 6中的表达及其与胰岛素分泌的关系。方法对M IN 6细胞进行不同浓度和不同时间的葡萄糖刺激胰岛素分泌的实验,酶联免疫吸附法测定细胞上清液中胰岛素分泌量,实时荧光定量PC R检测相应条件下PA-PLA1 m R N A的表达水平,线性相关分析PA-PLA1 m R N A表达水平与胰岛素分泌的相关性。结果在一定葡萄糖浓度范围内,PA-PLA1m R N A水平随着葡萄糖浓度的升高和刺激时间的延长而升高,并与细胞胰岛素分泌量呈明显的正相关。不同葡萄糖浓度和不同刺激时间的PA-PLA1 m R N A水平与胰岛素水平的相关系数r分别为0.89(P<0.01)和0.98(P<0.01)。结论小鼠分泌胰岛素细胞株M IN 6中PA-PLA1基因的表达与胰岛素分泌之间具有高度的正相关性。展开更多
文摘Objective To observe the changes of sciatic nerve conduction velocity （SNCV） and interleukin-1α （IL-1α）, phospholipase A2 （PLA2） and prostaglandin E2 （PGE2） in neucleus pulposus tissue in experimental rabbits with lumbar disc herniation （LDH） ; and discuss the mechanism of LDH treated with bloodletting on Wěizhōng （委中 BL40）. Methods 40 normal named control group, LDH model group, BL40 group New Zealand rabbits were randomized into 4 groups, and comparative point group. The experimental LDH model was prepared by the self-made LDH animal pathological modeling device. Bloodletting on BL40 or on comparative point was applied for 7 days. BL-410 physiological signal recorder was used to record SNCV. Enzyme-linked immunosorbent assay （ELISA） and radioimmunology methods were used to determine the contents of IL-1α and PLA2 in neucleus pulposus tissue and PGE2 in serum. Results In BL40 group, SNCV was significantly increased after treatment comparing with that before treatment （P〈0. 01 ）, and the difference in SNCV value before and after treatment was significantly bigger than that in model group and comparative point group （P〈0.05）. Bloodletting on BL40 reduced the contents of IL-1α and PLA2 in neucleus pulposus tissue and serum PGE2 in LDH rabbits, which indicated significant difference or very significant results in the comparison with model and comparative point groups （P〈0.01 or 0. 05）. Conclusion Bloodletting speeded up rapidly SNCV and reduced the contents of inflammatory factors, i.e. IL-1α, PLA2 and serum PGE2 in LDH rabbits, which indicated that alleviation of local inflammatory reaction is probably one of the mechanisms on LDH treated by bloodletting on BL40.
文摘目的研究亲磷酸酯酶A1(PA-PLA1)基因在小鼠分泌胰岛素细胞株M IN 6中的表达及其与胰岛素分泌的关系。方法对M IN 6细胞进行不同浓度和不同时间的葡萄糖刺激胰岛素分泌的实验,酶联免疫吸附法测定细胞上清液中胰岛素分泌量,实时荧光定量PC R检测相应条件下PA-PLA1 m R N A的表达水平,线性相关分析PA-PLA1 m R N A表达水平与胰岛素分泌的相关性。结果在一定葡萄糖浓度范围内,PA-PLA1m R N A水平随着葡萄糖浓度的升高和刺激时间的延长而升高,并与细胞胰岛素分泌量呈明显的正相关。不同葡萄糖浓度和不同刺激时间的PA-PLA1 m R N A水平与胰岛素水平的相关系数r分别为0.89(P<0.01)和0.98(P<0.01)。结论小鼠分泌胰岛素细胞株M IN 6中PA-PLA1基因的表达与胰岛素分泌之间具有高度的正相关性。
