Micro sate Hite instability（MSI） defines a subtype of colorectal cancer（CRC） with typical clinicopathologic characteristics. CRCs with MSI（MSI CRCs） frequently acquire accelerated carcinogenesis and 5-FU resista...Micro sate Hite instability（MSI） defines a subtype of colorectal cancer（CRC） with typical clinicopathologic characteristics. CRCs with MSI（MSI CRCs） frequently acquire accelerated carcinogenesis and 5-FU resistance, and the exact underlying mechanism remains incompletely understood. Our previous study has identified the microRNA（miRNA） expression profile in MSI CRCs. In this study, three miRNAs（miR-181 a, miR-135 a and miR-302 c） were validated by qRT-PCR to be dramatically decreased in 67 CRC samples. Proliferation and apoptosis assays demonstrated that miR-181 a/135 a/302 c function as tumor suppressors via repressing PLAG1/IGF2 signaling. Moreover, we presented compelling evidence that restoration of miR-181 a/135 a/302 c expression promoted sensitivity of MSI CRC cells to 5-FU treatment. miR-181 a/135 a/302 c exerted their effect on chemoresistance through attenuating PLAG1 expression. Notably, the hypermethylation status of MSI CRC accounts for the decrements of miR-181 a/135 a/302 c. Our results contribute to a better understanding of the mechanism of chemo？resistance in MSI CRCs, and provide a clue for digging the bio markers and therapeutic targets for CRC patients.展开更多
BACKGROUND Pleomorphic adenoma(PA)is the most common type of salivary gland tumor,and its common sites are parotid gland,sinus,nasal septum and cleft palate.PA is an uncommon benign type of tumor occurring in the brea...BACKGROUND Pleomorphic adenoma(PA)is the most common type of salivary gland tumor,and its common sites are parotid gland,sinus,nasal septum and cleft palate.PA is an uncommon benign type of tumor occurring in the breast,and there are few reports of cases in Asia.CASE SUMMARY An 84-year-old woman found a mass in the upper outer quadrant of the right breast>1 year ago.The patient underwent a right breast lumpectomy and sentinel lymph node biopsy.The pathological diagnosis was PA in the upper outer quadrant of the right breast,and the malignant component was malignant adenomyoepithelioma.The postoperative course was uneventful,and no chemotherapy was administered.At 18 mo of follow-up,the patient is alive and well,with no evidence of recurrent disease.CONCLUSION Patients with breast PA should first undergo extended excision of breast masses followed by pathological examination.If malignancy is confirmed or the surgical margin is positive,modified radical mastectomy should be performed.展开更多
基金supported by the National Key Research and Development Program of China (2016YFC1303501 and 2016YFC1303504)the National Natural Science Foundation of China (Nos. 81572914 and 81230061)
文摘Micro sate Hite instability（MSI） defines a subtype of colorectal cancer（CRC） with typical clinicopathologic characteristics. CRCs with MSI（MSI CRCs） frequently acquire accelerated carcinogenesis and 5-FU resistance, and the exact underlying mechanism remains incompletely understood. Our previous study has identified the microRNA（miRNA） expression profile in MSI CRCs. In this study, three miRNAs（miR-181 a, miR-135 a and miR-302 c） were validated by qRT-PCR to be dramatically decreased in 67 CRC samples. Proliferation and apoptosis assays demonstrated that miR-181 a/135 a/302 c function as tumor suppressors via repressing PLAG1/IGF2 signaling. Moreover, we presented compelling evidence that restoration of miR-181 a/135 a/302 c expression promoted sensitivity of MSI CRC cells to 5-FU treatment. miR-181 a/135 a/302 c exerted their effect on chemoresistance through attenuating PLAG1 expression. Notably, the hypermethylation status of MSI CRC accounts for the decrements of miR-181 a/135 a/302 c. Our results contribute to a better understanding of the mechanism of chemo？resistance in MSI CRCs, and provide a clue for digging the bio markers and therapeutic targets for CRC patients.
基金Supported by National Natural Science Foundation of China,No.81874063Natural Science Foundation of Anhui Province,No.2008085QH408.
文摘BACKGROUND Pleomorphic adenoma(PA)is the most common type of salivary gland tumor,and its common sites are parotid gland,sinus,nasal septum and cleft palate.PA is an uncommon benign type of tumor occurring in the breast,and there are few reports of cases in Asia.CASE SUMMARY An 84-year-old woman found a mass in the upper outer quadrant of the right breast>1 year ago.The patient underwent a right breast lumpectomy and sentinel lymph node biopsy.The pathological diagnosis was PA in the upper outer quadrant of the right breast,and the malignant component was malignant adenomyoepithelioma.The postoperative course was uneventful,and no chemotherapy was administered.At 18 mo of follow-up,the patient is alive and well,with no evidence of recurrent disease.CONCLUSION Patients with breast PA should first undergo extended excision of breast masses followed by pathological examination.If malignancy is confirmed or the surgical margin is positive,modified radical mastectomy should be performed.
文摘为了探寻影响杜寒杂交羊体尺性状的遗传标记,试验通过Multiplex SNaPshot技术对72只6月龄杜寒杂交羊的HMGA1、HMGA2、PLAG1和BMPR1B四个影响体型大小的候选基因中的20个SNPs位点进行了基因型分型,应用PLINK v1.07软件对单核苷酸多态性(SNPs)位点与体尺性状(体斜长、体高和管围)进行单点关联分析,利用Excel软件进行等位基因替代效应分析,并应用HaploView version 4.2软件进行单倍型构建及连锁不平衡分析。结果表明:除2个SNPs位点外,其他18个SNPs位点均存在突变基因型。HMGA1基因上游的rs405972023位点与体斜长和管围呈显著相关(P<0.05),rs404165907位点与体斜长呈显著相关(P<0.05);HMGA2基因第2内含子区域的rs399648873位点和该基因上游rs409565324位点与体高呈显著相关(P<0.05);位于PLAG1和BMPR1B基因及其周边区域的SNPs位点与杜寒杂交羊体尺性状未呈现显著相关(P>0.05)。HMGA1基因rs405972023位点TT基因型个体的体斜长极显著高于CT基因型个体(P<0.01),rs404165907位点AA基因型个体的体斜长显著高于CA和CC基因型个体(P<0.05)。HMGA1基因中的4个SNPs位点呈现强连锁,共构建出4种单倍型频率大于0.03的单倍型组合,TCAA、CTCG、TTAG、TTCG单倍型频率分别为0.479,0.318,0.096,0.071。说明rs404165907与rs405972023位点是可用于杜寒杂交羊体斜长育种的潜在分子标记。
