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用同步辐射X射线相反衬成像研究PM_(2.5)诱导的大鼠肺炎 被引量:1
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作者 童永彭 谈明光 +9 位作者 李燕 陈建敏 张桂林 胡宇光 蔡文利 许培诚 Jung Ho Je 宋伟民 蒋蓉芳 G.Margaritondo 《核技术》 CAS CSCD 北大核心 2004年第8期566-570,共5页
应用中流量采样器采集典型工业污染区空气动力学直径小于2.5 μm的颗粒物(PM2.5)。用电感耦合等离子体质谱(ICP-MS)分析其元素含量。以SD大鼠为实验对象,进行一次性气管灌注PM2 5溶液0.4 mL(3.0g/L,pH≈6.8)染毒,对照组用生理盐水。1、... 应用中流量采样器采集典型工业污染区空气动力学直径小于2.5 μm的颗粒物(PM2.5)。用电感耦合等离子体质谱(ICP-MS)分析其元素含量。以SD大鼠为实验对象,进行一次性气管灌注PM2 5溶液0.4 mL(3.0g/L,pH≈6.8)染毒,对照组用生理盐水。1、2天后观察肺组织病理改变并应用同步辐射X-射线相反衬成像研究肺组织的形态特征变化。结果显示,染了毒的大鼠肺具有肺炎的特征,相反衬成像观察到对应的染毒组肺中具有许多气泡,而对照组没有;随染毒后时间的增加气泡增多,对应的病理切片特征也显示炎症的增加,同时观察到,肺出血是在含有气泡部位出现,其间的肺泡也萎缩。结果提示了在颗粒物染毒诱导大鼠肺炎过程中,肺泡排气功能丧失导致局部空气滞留于肺泡中,进一步炎症引起肺出血继而导致肺细胞死亡的机制。 展开更多
关键词 同步辐射 X显微成像 相反衬 鼠肺 pm2.5毒性
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Physical and chemical characteristics of PM_(2.5) and its toxicity to human bronchial cells BEAS-2B in the winter and summer 被引量:2
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作者 Hui-hui ZHANG Zheng LI +5 位作者 Yu LIU Ping XINAG Xin-yi CUI Hui YE Bao-lan HU Li-ping LOU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2018年第4期317-326,共10页
With the increasing occurrence of haze during the summer, the physicochemical characteristics and toxicity differences in PM2.5 in different seasons are of great concern. Hangzhou is located in an area that has a subt... With the increasing occurrence of haze during the summer, the physicochemical characteristics and toxicity differences in PM2.5 in different seasons are of great concern. Hangzhou is located in an area that has a subtropical monsoon climate where the humidity is very high during both the summer and winter. However, there are limited studies on the seasonal differences in PM2.5 in these weather conditions. In this test, PM2.5 samples were collected in the winter and summer, the morphology and chemical composition of PM2.5 were analyzed, the toxicity of PM2.5 to human bronchial cells BEAS-2B was compared, and the correlation between PM2.5 toxicity and the chemical compo- sition was discussed. The results showed that during both the winter and summer, the main compounds in the PM2.5 samples were water-soluble ions, particularly SO4^2-, NO3^-, and NH4^+, followed by organic components, while heavy metals were present at lower levels. The higher the mass concentration of PM2.5, the greater its impact on cell viability and ROS levels. However, when the mass concentration of PM2.5 was similar, the water extraction from the summer samples showed a greater impact on BEAS-2B than that from the winter samples. The cytotoxicity of PM2.5 was closely associated with heavy metals and organic pollutants but less related to water-soluble ions. 展开更多
关键词 pm2.5 Seasonal difference Physical and chemical characteristics CYTOTOXICITY
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