本刊主编,武汉大学人民医院李艳教授（通讯作者）指导博士研究生陈占国（第一作者）等在《PLOS ONE》（2015,10（3） e0122530,IF：3.534）发表了题为“Development and validation of a 3-plex RT-qPCR assay for the simultaneous det...本刊主编,武汉大学人民医院李艳教授（通讯作者）指导博士研究生陈占国（第一作者）等在《PLOS ONE》（2015,10（3） e0122530,IF：3.534）发表了题为“Development and validation of a 3-plex RT-qPCR assay for the simultaneous detection and quantitation of the three PML-RARa fusion transcripts in acute promyelocytic leukemia”的研究论文,主要内容如下。展开更多
Objective Most acute promyelocytic leukemia cases are characterized by the PML-RARa fusion oncogene and low white cell counts in peripheral blood.Methods Based on the frequent overexpression of miR-125-family miRNAs i...Objective Most acute promyelocytic leukemia cases are characterized by the PML-RARa fusion oncogene and low white cell counts in peripheral blood.Methods Based on the frequent overexpression of miR-125-family miRNAs in acute promyelocytic leukemia,we examined the consequence of this phenomenon by using an inducible mouse model overexpressing human miR-125b.Results MiR-125b expression significantly accelerates PML-RARa-induced leukemogenesis,with the resultant induced leukemia being partially dependent on continued miR-125b overexpression.Interestingly,miR-125b expression led to low peripheral white cell counts to bone marrow blast percentage ratio,confirming the clinical observation in acute promyelocytic leukemia patients.Conclusion This study suggests that dysregulated miR-125b expression is actively involved in disease progression and pathophysiology of acute promyelocytic leukemia,indicating that targeting miR-125b may represent a new therapeutic option for acute promyelocytic leukemia.展开更多
文摘本刊主编,武汉大学人民医院李艳教授（通讯作者）指导博士研究生陈占国（第一作者）等在《PLOS ONE》（2015,10（3） e0122530,IF：3.534）发表了题为“Development and validation of a 3-plex RT-qPCR assay for the simultaneous detection and quantitation of the three PML-RARa fusion transcripts in acute promyelocytic leukemia”的研究论文,主要内容如下。
基金supported in part by NIH grants[R01CA149109,R01GM099811]Connecticut Regenerative Medicine Fund grant[15-RMB-YALE-06]National Clinical Research Center for Geriatric Diseases&Chinese PLA General Hospital grant[NCRCG-PLAGH-2022011]
文摘Objective Most acute promyelocytic leukemia cases are characterized by the PML-RARa fusion oncogene and low white cell counts in peripheral blood.Methods Based on the frequent overexpression of miR-125-family miRNAs in acute promyelocytic leukemia,we examined the consequence of this phenomenon by using an inducible mouse model overexpressing human miR-125b.Results MiR-125b expression significantly accelerates PML-RARa-induced leukemogenesis,with the resultant induced leukemia being partially dependent on continued miR-125b overexpression.Interestingly,miR-125b expression led to low peripheral white cell counts to bone marrow blast percentage ratio,confirming the clinical observation in acute promyelocytic leukemia patients.Conclusion This study suggests that dysregulated miR-125b expression is actively involved in disease progression and pathophysiology of acute promyelocytic leukemia,indicating that targeting miR-125b may represent a new therapeutic option for acute promyelocytic leukemia.
基金supported by the Natio-nal High-tech R&D Program(863 Program)(grant No.2006AA02A405)the Beijing Municipal Science&Tech-nology Commission"Health care of Beijing citizens"(2011)
文摘急性早幼粒细胞白血病(APL)接受维甲酸和砷剂诱导治疗早期的分子动力学及其临床意义尚不清楚。本研究对32例初治APL进行动态检测,利用实时定量PCR和间期荧光原位杂交(FISH)方法检测PML-RARα转录本水平(PML-RARα/ABL)和细胞遗传学。结果表明,诱导14 d时PML-RARα转录本水平比治疗前显著升高(40.10%和57.74%,P<0.01),诱导28 d和巩固治疗结束时PML-RARα转录本分别为:6.97%和0%。在诱导治疗14 d和28 d分别有65.62%和31.25%患者发生PML-RARα转录本增加。治疗前、诱导14 d和诱导28 d PML-RARα拷贝数/每个APL细胞为0.9,2.2,1.4(PML-RARα/ABL×2/APL细胞%)。中位随访时间为22个月,32例患者均无复发。结论:PML-RARα表达上调是急性早幼粒细胞白血病接受维甲酸和砷剂联合诱导治疗过程中一个普遍现象,对疾病预后无影响。
