利用机器人的理论和技术赋予电磁轴承以专业设计人员的"智慧",使它能够"独立"地"鉴别"将要"面对"的任何转子(或悬浮体)特点,再根据"已有的专业知识",包括"设计经验",对转...利用机器人的理论和技术赋予电磁轴承以专业设计人员的"智慧",使它能够"独立"地"鉴别"将要"面对"的任何转子(或悬浮体)特点,再根据"已有的专业知识",包括"设计经验",对转子(悬浮体)系统进行静、动特性分析与综合分析,"在线设计"出系统合适的控制参数,实现稳定的悬浮支承。本文针对在智能磁力轴承的实现过程中,智能磁力轴承的静态参数(也可称为固有参数或设计参数)及转子(悬浮体)的静态参数(如质量、质心、静载荷)的识别、存储和传输技术进行分析,并给出一种以计算机中的PnP(plug and play)技术为基础的实施方法。展开更多
Summary: To investigate the killing effect of PNP/MeP-dR suicide gene system on hepatoma cells, pcDNA3.0/PNP, an eukaryotic expression vector harboring E. coli PNP gene, was transfected into human hepatoma HepG2 cells...Summary: To investigate the killing effect of PNP/MeP-dR suicide gene system on hepatoma cells, pcDNA3.0/PNP, an eukaryotic expression vector harboring E. coli PNP gene, was transfected into human hepatoma HepG2 cells by liposome-mediated method. A HepG2 cell line with stable PNP gene expression, HepG2/PNP, was established with presence of G418 selection. The cell growth curves were determined with trypan blue staining. The sensitivity of HepG2/PNP to MeP-dR and bystander effects were assayed by MTT and FCM methods. The enzymatic activity of the product of PNP gene was determined by HPLC method. The cytotoxic effects of MeP-dR on HepG2/PNP cells were obvious (IC 50=4.5 μmol/L) and all HepG2/PNP cells were killed 4 days after the treatment with 100 μmol/L MeP-dR. In mixed cultures containing increasing percentages of HepG2/PNP cells, total population killing was demonstrated when HepG2/PNP cells accounted for as few as 5 % of all HepG2 cells 8 days after the treatment with 100μmol MeP-dR. High-pressure liquid chromatography (HPLC) demonstrated that the PNP enzyme could convert MeP-dR into 6-MP. PNP/MeP-dR suicide gene system had an advantage over traditional suicide gene systems for hepatoma gene therapy. Our e results suggest that high-level bystander effects of this system result in significant anti-tumor responses to hepatoma gene therapy, especially in vivo.展开更多
嘲讽者让你相信Plug and Play(“即插即用”)缺少新奇:插上板子,打开电源,观看你的微机自动进行系统配置。 让我给你讲一件可怕的事。几个月以前,我从一台微机中取出一块网络卡插到另一台微机中,这两台微机几乎是一模一样的,系统板也相...嘲讽者让你相信Plug and Play(“即插即用”)缺少新奇:插上板子,打开电源,观看你的微机自动进行系统配置。 让我给你讲一件可怕的事。几个月以前,我从一台微机中取出一块网络卡插到另一台微机中,这两台微机几乎是一模一样的,系统板也相同。在此之前,这两台机器工作都很正常,可是当我一插上网络卡,第二台微机就不能进行引导装入程序了。展开更多
文摘利用机器人的理论和技术赋予电磁轴承以专业设计人员的"智慧",使它能够"独立"地"鉴别"将要"面对"的任何转子(或悬浮体)特点,再根据"已有的专业知识",包括"设计经验",对转子(悬浮体)系统进行静、动特性分析与综合分析,"在线设计"出系统合适的控制参数,实现稳定的悬浮支承。本文针对在智能磁力轴承的实现过程中,智能磁力轴承的静态参数(也可称为固有参数或设计参数)及转子(悬浮体)的静态参数(如质量、质心、静载荷)的识别、存储和传输技术进行分析,并给出一种以计算机中的PnP(plug and play)技术为基础的实施方法。
基金This project was supported by a grant from the National Natural Science Foundation of China (No. 30330680).
文摘Summary: To investigate the killing effect of PNP/MeP-dR suicide gene system on hepatoma cells, pcDNA3.0/PNP, an eukaryotic expression vector harboring E. coli PNP gene, was transfected into human hepatoma HepG2 cells by liposome-mediated method. A HepG2 cell line with stable PNP gene expression, HepG2/PNP, was established with presence of G418 selection. The cell growth curves were determined with trypan blue staining. The sensitivity of HepG2/PNP to MeP-dR and bystander effects were assayed by MTT and FCM methods. The enzymatic activity of the product of PNP gene was determined by HPLC method. The cytotoxic effects of MeP-dR on HepG2/PNP cells were obvious (IC 50=4.5 μmol/L) and all HepG2/PNP cells were killed 4 days after the treatment with 100 μmol/L MeP-dR. In mixed cultures containing increasing percentages of HepG2/PNP cells, total population killing was demonstrated when HepG2/PNP cells accounted for as few as 5 % of all HepG2 cells 8 days after the treatment with 100μmol MeP-dR. High-pressure liquid chromatography (HPLC) demonstrated that the PNP enzyme could convert MeP-dR into 6-MP. PNP/MeP-dR suicide gene system had an advantage over traditional suicide gene systems for hepatoma gene therapy. Our e results suggest that high-level bystander effects of this system result in significant anti-tumor responses to hepatoma gene therapy, especially in vivo.
文摘嘲讽者让你相信Plug and Play(“即插即用”)缺少新奇:插上板子,打开电源,观看你的微机自动进行系统配置。 让我给你讲一件可怕的事。几个月以前,我从一台微机中取出一块网络卡插到另一台微机中,这两台微机几乎是一模一样的,系统板也相同。在此之前,这两台机器工作都很正常,可是当我一插上网络卡,第二台微机就不能进行引导装入程序了。
