Holographic quantitative structure activity relationship(HQSAR) is an emerging QSAR technique with the combined application of molecular hologram, which encoded the frequency of occurrence of various molecular fragme...Holographic quantitative structure activity relationship(HQSAR) is an emerging QSAR technique with the combined application of molecular hologram, which encoded the frequency of occurrence of various molecular fragment types, and the subsequent partial least squares(PLS) regression analysis. In this paper, the acute toxicity data to the guppy( poecilia reticulata) for a series of 56 substituted benzenes, phenols, aromatic amines and nitro aromatics were subjected and this resulted in a model with a high predictive ability. The influence of fragment size and fragment distinction parameters on the quality of HQSAR model was investigated. The robustness and predictive ability of the model were also validated by leave one out (LOO) cross validation procedure and external testing data set.展开更多
Objective:To describe the occurrence of various spinal deformations in a captive-bred wild line of Poecilia wingei(P.wingei).Methods:Fish belonging to a wild line of P.wingei caught from Laguna de Los Patos,Venezuela,...Objective:To describe the occurrence of various spinal deformations in a captive-bred wild line of Poecilia wingei(P.wingei).Methods:Fish belonging to a wild line of P.wingei caught from Laguna de Los Patos,Venezuela,were bred in an aquarium home-breeding system during a period of three years(2006-2009).The spinal curvature was observed to study spinal deformities in P.wingei.Results:Out of a total of 600 fish,22 showed different types of deformities(scoliosis,lordosis,kyphosis),with a higher incidence in females.Growth,swimming and breeding of deformed fish were generally normal.Conclusions:Possible causes for spinal curvature in fish are discussed on the basis of the current literature.While it is not possible to determine the exact cause(s)of spinal deformities observed in the present study,traumatic injuries,nutritional imbalances,genetic defects or a combination of these factors can be supposed to be involved in the pathogenesis of such lesions.展开更多
The present studies describe Chromosomal aberration effects of electrofishing, which were evaluated on Poecilia latipinna, located in Shat Al-Arab river in Al-garmma city (south of Iraq). The electrofishing derive use...The present studies describe Chromosomal aberration effects of electrofishing, which were evaluated on Poecilia latipinna, located in Shat Al-Arab river in Al-garmma city (south of Iraq). The electrofishing derive used in work is simulated to that used in the commercial fishing. The apparatus generates voltage ranged from 40 to 280 volts. Nine bearers of Poecilia latipinna sailfin molly fish in chromosomal analysis were divided into three treatments. The first were a control, the fishes of the second were exposed to 110 volts (10 seconds), and final groups were exposed to 110 volts (15 seconds). Mitotic index of the electrofishing with a control for each group decreased with increasing exposed time in somatic cell kidney tissue of Poecilia latipinna. The chromosome aberration analysis revealed a significant increase in the most frequent aberration per 150 metaphase in analyzed groups (1.33 in T1 groups, 39.33 in T2 groups) was chromosome break, fragment, range chromosome, Sticky chromosome mean, were higher in comparison to non exposed electrical shock fishing groups (control groups T1). At the same time, it showed a higher positive correlation of total chromosome aberration frequencies between T1 and T2 groups, while, all fishes died in T3 groups. According to our results, we represented the first record in Iraq.展开更多
The full length cDNA of a prion protein (PrP) encoding gene of guppy (Poecilia reticulata) and the corresponding ge-nomic DNA were cloned. The cDNA was 2245 bp in length and contained an open reading frame (ORF) of 15...The full length cDNA of a prion protein (PrP) encoding gene of guppy (Poecilia reticulata) and the corresponding ge-nomic DNA were cloned. The cDNA was 2245 bp in length and contained an open reading frame (ORF) of 1545 bp encoding a pro-tein of 515 amino acids,which held all typical structural characteristics of the functional PrP. The cloned genomic DNA fragment corresponding to the cDNA was 3720 bp in length,consisting of 2 introns and 2 exons. The 5’ untranslated region of cDNA origi-nated from the 2 exons,while the ORF originated from the second exon. Although the gene was transcribed in diverse tissues in-cluding brain,eye,liver,intestine,muscle and tail,its transcript was most abundant in the brain. In addition,the transcription of the gene was enhanced by 5 salinity,implying that it was associated with the response of guppy to saline stress.展开更多
Guppies( Poecilia reticulata) are considered a candidate model species for the identification and testing of endocrine-disrupting chemicals. Male guppies may be used to address the challenge of making potential linkag...Guppies( Poecilia reticulata) are considered a candidate model species for the identification and testing of endocrine-disrupting chemicals. Male guppies may be used to address the challenge of making potential linkages between alterations of biomarkers, both at the cellular and organ level, and adverse outcomes. In the present study, a predictive relationship between sex characteristics and reproductive output was observed in male guppies that underwent a long-term toxicity test with 0.5 μg/L 17 β-estradiol administered during the juvenile period. Radioimmunoassay and western blot analyses demonstrated that 17 β-estradiol exposure caused a significant increase in testicular 17 β-estradiol levels as well as the induction of exposure biomarkers, namely hepatic vitellogenin. Exposure to 17 β-estradiol also caused a significant decrease in testosterone levels, which consequently reduced the gonadosomatic index, sperm counts, and the coloration index. These changes of male sexual characteristics further translated into adverse influences on reproduction, as measured by a decrease in offspring production and survival rate. Our results suggest that the above-mentioned sexual characteristics of male guppies may be considered potential in vivo biomarkers of estrogen effects on reproduction.展开更多
Objective:To determine toxicity and safety margins of trichlorofon in different species as trichlorofon toxicity has a large variation in the susceptibility of different species.Methods:In this research,four viviparou...Objective:To determine toxicity and safety margins of trichlorofon in different species as trichlorofon toxicity has a large variation in the susceptibility of different species.Methods:In this research,four viviparous aquarium fish were exposed to trichlorofon for 96 h.LC_(50)of 24 h,48 h,72 h and 96 h were attained by probit analysis software SPSS Version 16.Fish samples were exposed to different concentrations of trichlorofon(5,10,20 and 40 mg/L)for 96 h and mortality were recorded,separately.Results:The 96 h LC_(50)of Poecilia latipinna,Poecilia reticulata a,Gambusia holbrooki and Xiphophorus helleri were 9.80,9.80,9.95 and 7.99 mg/L,respectively.Conclusions:According to the results of this research,LC_(50)values indicated Xiphophorus helleri was the most resistant and Gambusia holbrooki,with a few differences,was the most sensitive species compared with the fishes examined.展开更多
基于密度泛函理论,利用多氯二苯并对二噁英(PCDDs)和多氯二苯并呋喃(PCDFs)几何构型全优化而得的量子化学和延伸计算参数:前线轨道能、熵、无迹四极矩、分子绝对硬度、电负性和亲电指数等,对两类化合物在(虹鳉)脂-水间的平衡分配性质进...基于密度泛函理论,利用多氯二苯并对二噁英(PCDDs)和多氯二苯并呋喃(PCDFs)几何构型全优化而得的量子化学和延伸计算参数:前线轨道能、熵、无迹四极矩、分子绝对硬度、电负性和亲电指数等,对两类化合物在(虹鳉)脂-水间的平衡分配性质进行了定量化模型研究,并由多元线性回归分析(MLR)建立了二次函数型的定量结构-性质相关关系(QSPR):lgKbw=5.343-0.001(S-125.480)2-0.355(ω-3.239)2+0.006(Qzz-2.950)2-22.728(η-2.365)2.结果表明,模型具有较高的拟合优度与稳健性,其决定系数和交叉验证相关系数分别为0.943和0.908,且模型的内外部预测性能理想,总体质量明显优于SOFA(solubility parameter for fate analysis)计算结果,可用于未知脂-水分配性质的预测;PCDD/Fs脂-水平衡分配(系数)主要与分子体积以及由电荷分布决定的芳烃分子间相互作用有关,也受潜在的生物转化和分子反应性的影响.展开更多
文摘Holographic quantitative structure activity relationship(HQSAR) is an emerging QSAR technique with the combined application of molecular hologram, which encoded the frequency of occurrence of various molecular fragment types, and the subsequent partial least squares(PLS) regression analysis. In this paper, the acute toxicity data to the guppy( poecilia reticulata) for a series of 56 substituted benzenes, phenols, aromatic amines and nitro aromatics were subjected and this resulted in a model with a high predictive ability. The influence of fragment size and fragment distinction parameters on the quality of HQSAR model was investigated. The robustness and predictive ability of the model were also validated by leave one out (LOO) cross validation procedure and external testing data set.
基金Supported by a grant from University of Teramo(Research Projects 60%/2009)
文摘Objective:To describe the occurrence of various spinal deformations in a captive-bred wild line of Poecilia wingei(P.wingei).Methods:Fish belonging to a wild line of P.wingei caught from Laguna de Los Patos,Venezuela,were bred in an aquarium home-breeding system during a period of three years(2006-2009).The spinal curvature was observed to study spinal deformities in P.wingei.Results:Out of a total of 600 fish,22 showed different types of deformities(scoliosis,lordosis,kyphosis),with a higher incidence in females.Growth,swimming and breeding of deformed fish were generally normal.Conclusions:Possible causes for spinal curvature in fish are discussed on the basis of the current literature.While it is not possible to determine the exact cause(s)of spinal deformities observed in the present study,traumatic injuries,nutritional imbalances,genetic defects or a combination of these factors can be supposed to be involved in the pathogenesis of such lesions.
文摘The present studies describe Chromosomal aberration effects of electrofishing, which were evaluated on Poecilia latipinna, located in Shat Al-Arab river in Al-garmma city (south of Iraq). The electrofishing derive used in work is simulated to that used in the commercial fishing. The apparatus generates voltage ranged from 40 to 280 volts. Nine bearers of Poecilia latipinna sailfin molly fish in chromosomal analysis were divided into three treatments. The first were a control, the fishes of the second were exposed to 110 volts (10 seconds), and final groups were exposed to 110 volts (15 seconds). Mitotic index of the electrofishing with a control for each group decreased with increasing exposed time in somatic cell kidney tissue of Poecilia latipinna. The chromosome aberration analysis revealed a significant increase in the most frequent aberration per 150 metaphase in analyzed groups (1.33 in T1 groups, 39.33 in T2 groups) was chromosome break, fragment, range chromosome, Sticky chromosome mean, were higher in comparison to non exposed electrical shock fishing groups (control groups T1). At the same time, it showed a higher positive correlation of total chromosome aberration frequencies between T1 and T2 groups, while, all fishes died in T3 groups. According to our results, we represented the first record in Iraq.
基金the Key Laboratory of Freshwater Germplasm and Biotechnology of Chinese Ministry of Agriculture,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences (LFB20070601)the Key Laboratory of Mariculture of Chinese Ministry of Education,Ocean University of ChinaNational High Technology Research and Development Program of China (2007AA09Z427)
文摘The full length cDNA of a prion protein (PrP) encoding gene of guppy (Poecilia reticulata) and the corresponding ge-nomic DNA were cloned. The cDNA was 2245 bp in length and contained an open reading frame (ORF) of 1545 bp encoding a pro-tein of 515 amino acids,which held all typical structural characteristics of the functional PrP. The cloned genomic DNA fragment corresponding to the cDNA was 3720 bp in length,consisting of 2 introns and 2 exons. The 5’ untranslated region of cDNA origi-nated from the 2 exons,while the ORF originated from the second exon. Although the gene was transcribed in diverse tissues in-cluding brain,eye,liver,intestine,muscle and tail,its transcript was most abundant in the brain. In addition,the transcription of the gene was enhanced by 5 salinity,implying that it was associated with the response of guppy to saline stress.
基金Supported by the National Natural Science Foundation of China(No.31202001)the Natural Science Foundation of Shandong Province,China(No.ZR2012CQ010)
文摘Guppies( Poecilia reticulata) are considered a candidate model species for the identification and testing of endocrine-disrupting chemicals. Male guppies may be used to address the challenge of making potential linkages between alterations of biomarkers, both at the cellular and organ level, and adverse outcomes. In the present study, a predictive relationship between sex characteristics and reproductive output was observed in male guppies that underwent a long-term toxicity test with 0.5 μg/L 17 β-estradiol administered during the juvenile period. Radioimmunoassay and western blot analyses demonstrated that 17 β-estradiol exposure caused a significant increase in testicular 17 β-estradiol levels as well as the induction of exposure biomarkers, namely hepatic vitellogenin. Exposure to 17 β-estradiol also caused a significant decrease in testosterone levels, which consequently reduced the gonadosomatic index, sperm counts, and the coloration index. These changes of male sexual characteristics further translated into adverse influences on reproduction, as measured by a decrease in offspring production and survival rate. Our results suggest that the above-mentioned sexual characteristics of male guppies may be considered potential in vivo biomarkers of estrogen effects on reproduction.
基金Supported by the Gorgan University of Agriculture Science and Natural Resource(Grant No.6177515-5).
文摘Objective:To determine toxicity and safety margins of trichlorofon in different species as trichlorofon toxicity has a large variation in the susceptibility of different species.Methods:In this research,four viviparous aquarium fish were exposed to trichlorofon for 96 h.LC_(50)of 24 h,48 h,72 h and 96 h were attained by probit analysis software SPSS Version 16.Fish samples were exposed to different concentrations of trichlorofon(5,10,20 and 40 mg/L)for 96 h and mortality were recorded,separately.Results:The 96 h LC_(50)of Poecilia latipinna,Poecilia reticulata a,Gambusia holbrooki and Xiphophorus helleri were 9.80,9.80,9.95 and 7.99 mg/L,respectively.Conclusions:According to the results of this research,LC_(50)values indicated Xiphophorus helleri was the most resistant and Gambusia holbrooki,with a few differences,was the most sensitive species compared with the fishes examined.
文摘基于密度泛函理论,利用多氯二苯并对二噁英(PCDDs)和多氯二苯并呋喃(PCDFs)几何构型全优化而得的量子化学和延伸计算参数:前线轨道能、熵、无迹四极矩、分子绝对硬度、电负性和亲电指数等,对两类化合物在(虹鳉)脂-水间的平衡分配性质进行了定量化模型研究,并由多元线性回归分析(MLR)建立了二次函数型的定量结构-性质相关关系(QSPR):lgKbw=5.343-0.001(S-125.480)2-0.355(ω-3.239)2+0.006(Qzz-2.950)2-22.728(η-2.365)2.结果表明,模型具有较高的拟合优度与稳健性,其决定系数和交叉验证相关系数分别为0.943和0.908,且模型的内外部预测性能理想,总体质量明显优于SOFA(solubility parameter for fate analysis)计算结果,可用于未知脂-水分配性质的预测;PCDD/Fs脂-水平衡分配(系数)主要与分子体积以及由电荷分布决定的芳烃分子间相互作用有关,也受潜在的生物转化和分子反应性的影响.
