Macroalgae in a littoral zone are inevitably exposed to UV-B irradiance.We analyzed the effects of UV-B on isoenzyme patterns and activities of superoxide dismutase(SOD),peroxidase(POX),catalase(CAT),and ascorbate per...Macroalgae in a littoral zone are inevitably exposed to UV-B irradiance.We analyzed the effects of UV-B on isoenzyme patterns and activities of superoxide dismutase(SOD),peroxidase(POX),catalase(CAT),and ascorbate peroxidase(APX) of red algae Grateloupia filicina(Lamour.) C.Agardh.The activities of SOD,CAT,and APX changed in response to UV-B in a time- and dose-dependent manner.POX activity increased significantly under all three UV-B treatments.The enzymatic assay showed three distinct bands of SODⅠ(Mn-SOD),SODⅡ(Fe-SOD),and SODⅢ(CuZn-SOD) under a low(Luv) and medium(Muv) dose of UV-B irradiation,while SODⅠ and SODⅢ activities decreased significantly when exposed to a high dose of UV-B irradiation(Huv).The activity of POX isoenzymes increased significantly after exposure to UV-B,which is consistent with the total activity.In addition,a clear decrease in activity of CATIV was detected in response to all the three doses of UV treatments.Some bands of APX isoenzyme were also clearly influenced by UV-B irradiation.Correspondingly,the daily growth rate declined under all the three exposure doses,and was especially significant under Muv and Huv treatments.These data suggest that,although the protection mechanisms of antioxidant defense system are partly inducible by UV-B to prevent the damage,G.filicina has incomplete tolerance to higher UV-B irradiation stress.展开更多
基金Supported by the National Natural Science Foundation of China(No.31300326)the Natural Science Foundation of Shandong Province(Nos.ZR2010DQ007,ZR2011CM006)
文摘Macroalgae in a littoral zone are inevitably exposed to UV-B irradiance.We analyzed the effects of UV-B on isoenzyme patterns and activities of superoxide dismutase(SOD),peroxidase(POX),catalase(CAT),and ascorbate peroxidase(APX) of red algae Grateloupia filicina(Lamour.) C.Agardh.The activities of SOD,CAT,and APX changed in response to UV-B in a time- and dose-dependent manner.POX activity increased significantly under all three UV-B treatments.The enzymatic assay showed three distinct bands of SODⅠ(Mn-SOD),SODⅡ(Fe-SOD),and SODⅢ(CuZn-SOD) under a low(Luv) and medium(Muv) dose of UV-B irradiation,while SODⅠ and SODⅢ activities decreased significantly when exposed to a high dose of UV-B irradiation(Huv).The activity of POX isoenzymes increased significantly after exposure to UV-B,which is consistent with the total activity.In addition,a clear decrease in activity of CATIV was detected in response to all the three doses of UV treatments.Some bands of APX isoenzyme were also clearly influenced by UV-B irradiation.Correspondingly,the daily growth rate declined under all the three exposure doses,and was especially significant under Muv and Huv treatments.These data suggest that,although the protection mechanisms of antioxidant defense system are partly inducible by UV-B to prevent the damage,G.filicina has incomplete tolerance to higher UV-B irradiation stress.
