The speckle-type POZ protein （SPOP） is a tumor suppressor in prostate cancer （PCa）. SPOP somatic mutations have been reported in up to 15% of PCa of those of European descent. However, the genetic roles of SPOP in...The speckle-type POZ protein （SPOP） is a tumor suppressor in prostate cancer （PCa）. SPOP somatic mutations have been reported in up to 15% of PCa of those of European descent. However, the genetic roles of SPOP in African American （AA）-PCa are currently unknown. We sequenced the SPOP gene to identify somatic mutations in 49 AA prostate tumors and identified three missense mutations （p.Y87C, p.F102S, and p.G111E） in five AA prostate tumors （10%） and one synonymous variant （p.11061） in one tumor. Intriguingly, all of mutations and variants clustered in exon six, and all of the mutations altered conserved amino acids. Moreover, two mutations （p.F102S and p.G111E） have only been identified in AA-PCa to date. Quantitative real-time polymerase chain reaction analysis showed a lower level of SPOP expression in tumors carrying SPOP mutations than their matched normal prostate tissues. In addition, SPOP mutations and novel variants were detected in 5 of 27 aggressive PCa and one of 22 less aggressive PCa （P 〈 0.05）. Further studies with increased sample size are needed to validate the clinicopathological significance of these SPOP mutations in AA-PCa.展开更多
Peripheral motor and sensory neuropathies are diseases with different etiologies emerging from genetic disorders,diabetes,infection or inflammation,paraneoplastic damage or intoxications including alcohol abuse（Marty...Peripheral motor and sensory neuropathies are diseases with different etiologies emerging from genetic disorders,diabetes,infection or inflammation,paraneoplastic damage or intoxications including alcohol abuse（Martyn and Hughes,1997）.展开更多
In this study, a recombinant pET28c-gBTB/POZ was constructed by cloning the sequence of the BTB/POZ domain of the zebrafish gcl （germ cell-less） into the expression vector pET28c, and pET28c-gBTB/POZ was transformed...In this study, a recombinant pET28c-gBTB/POZ was constructed by cloning the sequence of the BTB/POZ domain of the zebrafish gcl （germ cell-less） into the expression vector pET28c, and pET28c-gBTB/POZ was transformed into BL21（DE3） pLysS strain to express the fusion protein for the preparation of antibody. Polyclonal-antibody against the GCL-BTB/POZ domain was prepared by immunizing rabbit with the fusion protein, and the Western Blot and immuno-histochemical analysis were performed to detect the quantity of the polyclonal-antibody. The result indicates that the polyclonal-antibodies were of good quantity and specification. Further studies will be performed to demonstrate the function and expression pattern of the GCL protein during the development process of zebrafish with the polyclonal-antibody.展开更多
目的:探讨BTB/POZ结构域蛋白7(BTBD7)假基因1(BTBD7P1)在肝细胞癌(HCC)中的表达及功能。方法:检测106例配对的HCC组织与癌旁组织标本中BTBD7P1 m RNA的表达,分析BTBD7P1m RNA表达与HCC患者临床病理特征及预后的关系。用BTBD7P1过表达慢...目的:探讨BTB/POZ结构域蛋白7(BTBD7)假基因1(BTBD7P1)在肝细胞癌(HCC)中的表达及功能。方法:检测106例配对的HCC组织与癌旁组织标本中BTBD7P1 m RNA的表达,分析BTBD7P1m RNA表达与HCC患者临床病理特征及预后的关系。用BTBD7P1过表达慢病毒载体转染HCC细胞系Bel7404后,检测细胞增殖率以及BTBD7 m RNA与蛋白的表达。结果:HCC组织中BTBD7P1相对表达量明显低于癌旁组织为(0.71 vs.2.14,P<0.05);BTBD7P1m RNA低表达与肿瘤大小、卫星灶、分化程度、静脉血管侵犯、出血坏死、HCC分期明显有关(均P<0.05);BTBD7P1 m RNA低表达患者的1、3、5年总体生存率及无瘤生存率均明显低于BTBD7P1m RNA高表达患者(均P<0.05)。与转染空载体质粒的对照组Bel7404细胞比较,转染BTBD7P1过表达慢病毒载体的Bel7404细胞,细胞增殖能力明显减低,BTBD7 m RNA表达明显下调(均P<0.05),但BTBD7蛋白表达无明显变化(P>0.05)。结论:BTBD7P1可能在m RNA水平对亲本基因BTBD7表达进行调控,从而参与了HCC发生与发展。展开更多
目的研究BTB/POZ(broad complex,tramtrack and bric a brac/poxviruses and zinc finger)基因对乳腺癌细胞系增殖能力的影响。方法以人脾脏c DNA文库为模板,p CMV-HA-Vector为载体,构建真核表达载体p CMV-HA-BPOZ;免疫印迹实验鉴定重...目的研究BTB/POZ(broad complex,tramtrack and bric a brac/poxviruses and zinc finger)基因对乳腺癌细胞系增殖能力的影响。方法以人脾脏c DNA文库为模板,p CMV-HA-Vector为载体,构建真核表达载体p CMV-HA-BPOZ;免疫印迹实验鉴定重组蛋白p CMV-HA-BPOZ的表达;细胞生长实验检测BPOZ对细胞增殖能力的影响;平板克隆实验检测BPOZ对细胞生长能力的影响。结果免疫印迹实验结果证实,构建的真核表达载体p CMV-HA-BPOZ能正确表达;过表达HA-BPOZ可显著抑制MCF7和ZR-75-1细胞的增殖和生长。结论成功构建了真核表达载体p CMV-HA-BPOZ,并在细胞中成功表达;BPOZ可明显抑制MCF7和ZR-75-1细胞的增殖和生长能力,为进一步研究BPOZ在乳腺癌发生发展中的作用打下基础。展开更多
文摘The speckle-type POZ protein （SPOP） is a tumor suppressor in prostate cancer （PCa）. SPOP somatic mutations have been reported in up to 15% of PCa of those of European descent. However, the genetic roles of SPOP in African American （AA）-PCa are currently unknown. We sequenced the SPOP gene to identify somatic mutations in 49 AA prostate tumors and identified three missense mutations （p.Y87C, p.F102S, and p.G111E） in five AA prostate tumors （10%） and one synonymous variant （p.11061） in one tumor. Intriguingly, all of mutations and variants clustered in exon six, and all of the mutations altered conserved amino acids. Moreover, two mutations （p.F102S and p.G111E） have only been identified in AA-PCa to date. Quantitative real-time polymerase chain reaction analysis showed a lower level of SPOP expression in tumors carrying SPOP mutations than their matched normal prostate tissues. In addition, SPOP mutations and novel variants were detected in 5 of 27 aggressive PCa and one of 22 less aggressive PCa （P 〈 0.05）. Further studies with increased sample size are needed to validate the clinicopathological significance of these SPOP mutations in AA-PCa.
基金supported by Deutsche Forschungsgemeinschaft(DFG grant EL125/6-1)
文摘Peripheral motor and sensory neuropathies are diseases with different etiologies emerging from genetic disorders,diabetes,infection or inflammation,paraneoplastic damage or intoxications including alcohol abuse（Martyn and Hughes,1997）.
基金Supported by the National Natural Science Foundation of China (30570968, 30370744)
文摘In this study, a recombinant pET28c-gBTB/POZ was constructed by cloning the sequence of the BTB/POZ domain of the zebrafish gcl （germ cell-less） into the expression vector pET28c, and pET28c-gBTB/POZ was transformed into BL21（DE3） pLysS strain to express the fusion protein for the preparation of antibody. Polyclonal-antibody against the GCL-BTB/POZ domain was prepared by immunizing rabbit with the fusion protein, and the Western Blot and immuno-histochemical analysis were performed to detect the quantity of the polyclonal-antibody. The result indicates that the polyclonal-antibodies were of good quantity and specification. Further studies will be performed to demonstrate the function and expression pattern of the GCL protein during the development process of zebrafish with the polyclonal-antibody.
文摘目的:探讨BTB/POZ结构域蛋白7(BTBD7)假基因1(BTBD7P1)在肝细胞癌(HCC)中的表达及功能。方法:检测106例配对的HCC组织与癌旁组织标本中BTBD7P1 m RNA的表达,分析BTBD7P1m RNA表达与HCC患者临床病理特征及预后的关系。用BTBD7P1过表达慢病毒载体转染HCC细胞系Bel7404后,检测细胞增殖率以及BTBD7 m RNA与蛋白的表达。结果:HCC组织中BTBD7P1相对表达量明显低于癌旁组织为(0.71 vs.2.14,P<0.05);BTBD7P1m RNA低表达与肿瘤大小、卫星灶、分化程度、静脉血管侵犯、出血坏死、HCC分期明显有关(均P<0.05);BTBD7P1 m RNA低表达患者的1、3、5年总体生存率及无瘤生存率均明显低于BTBD7P1m RNA高表达患者(均P<0.05)。与转染空载体质粒的对照组Bel7404细胞比较,转染BTBD7P1过表达慢病毒载体的Bel7404细胞,细胞增殖能力明显减低,BTBD7 m RNA表达明显下调(均P<0.05),但BTBD7蛋白表达无明显变化(P>0.05)。结论:BTBD7P1可能在m RNA水平对亲本基因BTBD7表达进行调控,从而参与了HCC发生与发展。
文摘目的研究BTB/POZ(broad complex,tramtrack and bric a brac/poxviruses and zinc finger)基因对乳腺癌细胞系增殖能力的影响。方法以人脾脏c DNA文库为模板,p CMV-HA-Vector为载体,构建真核表达载体p CMV-HA-BPOZ;免疫印迹实验鉴定重组蛋白p CMV-HA-BPOZ的表达;细胞生长实验检测BPOZ对细胞增殖能力的影响;平板克隆实验检测BPOZ对细胞生长能力的影响。结果免疫印迹实验结果证实,构建的真核表达载体p CMV-HA-BPOZ能正确表达;过表达HA-BPOZ可显著抑制MCF7和ZR-75-1细胞的增殖和生长。结论成功构建了真核表达载体p CMV-HA-BPOZ,并在细胞中成功表达;BPOZ可明显抑制MCF7和ZR-75-1细胞的增殖和生长能力,为进一步研究BPOZ在乳腺癌发生发展中的作用打下基础。
