To examine the relationship between expression of the c fosmRNA, ppENKmRNA, iNOSmRNA, iNOS activity and acupuncture analgesia, the acupuncture effect on expression of them in the mouse spinal cord was studied. 20 BALB...To examine the relationship between expression of the c fosmRNA, ppENKmRNA, iNOSmRNA, iNOS activity and acupuncture analgesia, the acupuncture effect on expression of them in the mouse spinal cord was studied. 20 BALB/C mice were randomly divided into 2 groups: a. the acupuncture group treated with 5 Hz electroacupuncture(EA); b. the control group treated with no EA. The gene expression of c fos, ppENK and iNOS in the spinal cord was detected by using in situ hybridization and RNA dot blot techniques. The iNOS activity was detected by using histochemistry and protein dot blot. The dot blot signals were scanned for statistical analysis. The results showed that the hybridization signals appeared bluish violet color. The c fos signals were localized in the laminae I-II and V-VII, the ppEMK weak signals localized in the superficial lamina and the iNOS signals localized in the cytoplasm of astrocytes of the mouse spinal cord. The pain threshold and all of the dot blot signals were enhanced in the acupuncture group when compared to that in the control group, P<0.05. In the acupuncture group, the intensity of expression of c fosmRNA, ppENKmRNA, iNOSmRNA and iNOS activity was positively correlated with analgesia effect respectively, P<0.05-0.01. The enhanced expression of iNOSmRNA and iNOS suggests that the spinal glia may play a more active role in synaptic function to cause analgesia induced by acupuncture stimulation. Besides, there was a positive correlation in alteration between spinal c fosmRNA and ppENK or c fosmRNA and iNOSmRNA, suggesting that c fos may be involved in ppENKmRNA transcription and c fos and iNOS may have common transactivators.展开更多
To investigate the relation between acupuncture stimulation and cellular immunity and the relation between the expression of cfosmRNA and ppENKmRNA or MEKIR and DynIR, the effect of acupuncture on the circulating lymp...To investigate the relation between acupuncture stimulation and cellular immunity and the relation between the expression of cfosmRNA and ppENKmRNA or MEKIR and DynIR, the effect of acupuncture on the circulating lymphocyte cfosmRNA, ppENKmRNA, MEKIR and DynIR was studied. 20 BALB/C mice were randomly divided into 2 groups: a) acupuncture group, treated with 5 Hz electroacupuncture(EA); b) control group, treated with restraint but no EA. The mouse pain threshold was detected by K+ ionophoresis before and after EA or restraining. The mouse lymphocyte cfosmRNA and ppENKmRNA were demonstrated by in situ hybridization and RNA dot blot. The mouse lymphocyte MEKIR and DynIR were detected by immunohistochemistry and protein dot blot. The dot blot signals were scanned for statistical analysis. The results showed that all the signals offosmRNA, ppENKmRNA, MEKIR and DynIR were localized in the lymphocyte cytoplasma and stronger in the acupuncture group than that in the control group(P<0.05). All of the signals were positively correlated with the analgesic effect in the EA group(P<0.05-0.01). Besides, there was a positive correlation on alteration between cfosmRNA and ppENKmRNA or between cfosmRNA and DynIR(P<0.05), suggesting that cFos may be involved in ppE or ppD gene transcription in the mouse lymphocyte.展开更多
Novel high temperature-resistant coatings with high mechanical strength and thermal-insulating performance were prepared with poly(ether nitrile ketone)(PPENK)resin as matrix and hollow glass microspheres(HGMs)as ther...Novel high temperature-resistant coatings with high mechanical strength and thermal-insulating performance were prepared with poly(ether nitrile ketone)(PPENK)resin as matrix and hollow glass microspheres(HGMs)as thermal-insulating filler.The corresponding mechanical and thermal-insulating study indicated that the mechanical properties of the coatings decreased with the increase of HGM content,and were improved after surface modification of HGM by KH570 resulting in enhancement of interaction between HGM and PPENK resin.The thermal conductivity of HGM/PPENK thermal-insulating coating decreased with the increase of HGM content and coating thickness,along with the decrease of the true density.It also showed slight increase trend due to HGMs surface modification.The HGM/PPENK coating filled with modified HGMs showed better thermal resistance than that of unmodified HGM/PPENK coating.The thermal decomposition temperature at 5%weight loss of the coating containing modified HGMs was 10°C lower than that of pure PPENK,and 40°C higher than that of neat HGM/PPENK coating.The coating exhibited commendable appearance after 400°C for 30 min.The merits of HGM/PPENK-based thermal coatings obviously demonstrated promising prospect in thermal protection fields.展开更多
文摘To examine the relationship between expression of the c fosmRNA, ppENKmRNA, iNOSmRNA, iNOS activity and acupuncture analgesia, the acupuncture effect on expression of them in the mouse spinal cord was studied. 20 BALB/C mice were randomly divided into 2 groups: a. the acupuncture group treated with 5 Hz electroacupuncture(EA); b. the control group treated with no EA. The gene expression of c fos, ppENK and iNOS in the spinal cord was detected by using in situ hybridization and RNA dot blot techniques. The iNOS activity was detected by using histochemistry and protein dot blot. The dot blot signals were scanned for statistical analysis. The results showed that the hybridization signals appeared bluish violet color. The c fos signals were localized in the laminae I-II and V-VII, the ppEMK weak signals localized in the superficial lamina and the iNOS signals localized in the cytoplasm of astrocytes of the mouse spinal cord. The pain threshold and all of the dot blot signals were enhanced in the acupuncture group when compared to that in the control group, P<0.05. In the acupuncture group, the intensity of expression of c fosmRNA, ppENKmRNA, iNOSmRNA and iNOS activity was positively correlated with analgesia effect respectively, P<0.05-0.01. The enhanced expression of iNOSmRNA and iNOS suggests that the spinal glia may play a more active role in synaptic function to cause analgesia induced by acupuncture stimulation. Besides, there was a positive correlation in alteration between spinal c fosmRNA and ppENK or c fosmRNA and iNOSmRNA, suggesting that c fos may be involved in ppENKmRNA transcription and c fos and iNOS may have common transactivators.
文摘To investigate the relation between acupuncture stimulation and cellular immunity and the relation between the expression of cfosmRNA and ppENKmRNA or MEKIR and DynIR, the effect of acupuncture on the circulating lymphocyte cfosmRNA, ppENKmRNA, MEKIR and DynIR was studied. 20 BALB/C mice were randomly divided into 2 groups: a) acupuncture group, treated with 5 Hz electroacupuncture(EA); b) control group, treated with restraint but no EA. The mouse pain threshold was detected by K+ ionophoresis before and after EA or restraining. The mouse lymphocyte cfosmRNA and ppENKmRNA were demonstrated by in situ hybridization and RNA dot blot. The mouse lymphocyte MEKIR and DynIR were detected by immunohistochemistry and protein dot blot. The dot blot signals were scanned for statistical analysis. The results showed that all the signals offosmRNA, ppENKmRNA, MEKIR and DynIR were localized in the lymphocyte cytoplasma and stronger in the acupuncture group than that in the control group(P<0.05). All of the signals were positively correlated with the analgesic effect in the EA group(P<0.05-0.01). Besides, there was a positive correlation on alteration between cfosmRNA and ppENKmRNA or between cfosmRNA and DynIR(P<0.05), suggesting that cFos may be involved in ppE or ppD gene transcription in the mouse lymphocyte.
基金by the National Natural Science Foundation of China(No.51903028)China Postdoctoral Science Foundation(No.2017M611217).
文摘Novel high temperature-resistant coatings with high mechanical strength and thermal-insulating performance were prepared with poly(ether nitrile ketone)(PPENK)resin as matrix and hollow glass microspheres(HGMs)as thermal-insulating filler.The corresponding mechanical and thermal-insulating study indicated that the mechanical properties of the coatings decreased with the increase of HGM content,and were improved after surface modification of HGM by KH570 resulting in enhancement of interaction between HGM and PPENK resin.The thermal conductivity of HGM/PPENK thermal-insulating coating decreased with the increase of HGM content and coating thickness,along with the decrease of the true density.It also showed slight increase trend due to HGMs surface modification.The HGM/PPENK coating filled with modified HGMs showed better thermal resistance than that of unmodified HGM/PPENK coating.The thermal decomposition temperature at 5%weight loss of the coating containing modified HGMs was 10°C lower than that of pure PPENK,and 40°C higher than that of neat HGM/PPENK coating.The coating exhibited commendable appearance after 400°C for 30 min.The merits of HGM/PPENK-based thermal coatings obviously demonstrated promising prospect in thermal protection fields.
