金花菜叶绿体基因组特征及密码子偏好性分析

本研究对金花菜(Medicago polymorpha,JHC,江苏)叶绿体基因组进行BGISEQ-500平台测序,对其基因进行Noveplastys组装和GeSeq注释,最后通过MISA、REPuter、Gview、VISTA tools、IRscope和DNADnaSP6.0等软件对其序列特征、基因种类、密码子偏好性以及系统发育进行分析。结果表明,JHC叶绿体基因组大小为124163 bp,无四分体结构,基因组总GC含量为34.09%,共注释出111个基因(76个CDS基因、30个tRNA基因、4个rRNA基因、1个假基因)、91个SSR位点和148个长重复序列;JHC叶绿体基因组的第三位密码子偏好以A和U作为末尾碱基,UUA、GCU、UAA、ACU、CCU、GAA、GUA、AUU、UGU、GGU、UUU、CUU为JHC叶绿体基因组的最优密码子;JHC与MZ772862(云南)亲缘关系较近,与MW971560(美国)、NC_042848(美国)关系较远,表明中国金花菜与美国金花菜的起源存在差异。

基于精益生产的工厂产线设计方法

为解决制造业生产线产出低、浪费大等问题,将精益生产技术应用到企业产线设计中,在产线设计之初规避资源浪费问题,使企业生产产线最合理化。运用精益生产方法为基础,以满足市场需求为原则,通过产品PQ-PR分析,对工厂产线进行分族定义,并结合精益改善方法,对产线设计进行优化。通过对A公司的终端车间产线进行精益设计,结果表明基于产品分析设计的精益产线相比于传统产线,在人均产出、生产效率等方面有显著提升,大大降低了生产过程的人力物力等浪费。

Error analysis and distribution of the driving mechanism for large spherical radio telescope active reflector

In order to reduce the cost,3-PRS mechanism is introduced into the application of supporting theactive reflector unit of large radio telescope.The kinematic model of 3-PRS mechanism with rotationaljoint errors is derived to solve the error problem in actual engineering application.Then based on the errormodel,inverse and forward kinematics are analyzed.Because the solutions can not be analytically ex-pressed,a numerical method is applied.Afterwards,the parasitic motion errors are analyzed using searchmethod and empirical formulas of the maximum parasitic motion error are put forward.Finally,the toler-ance is distributed using empirical formulas to avoid interference between adjacent reflector units.Theanalyses provide a theoretical basis for the design and installation of large radio telescope active reflector.