PRKAG3是编码一磷酸腺苷激活蛋白激酶(AMP-activated protein kinase,AMPK)γ3亚基的基因,是近年来确定的一个影响猪肉pH值、肉色以及系水力的主效基因,其突变R200Q与过量肌糖原含量有关,被认为是汉普夏猪发生RN-突变的根本原因。本研究...PRKAG3是编码一磷酸腺苷激活蛋白激酶(AMP-activated protein kinase,AMPK)γ3亚基的基因,是近年来确定的一个影响猪肉pH值、肉色以及系水力的主效基因,其突变R200Q与过量肌糖原含量有关,被认为是汉普夏猪发生RN-突变的根本原因。本研究以-βactin作为内参,用半定量RT-PCR方法测定雅南猪和杜×长×大三元杂交猪(DLY猪)PRKAG3基因在心脏、肝脏、肾和骨骼肌中表达差异,并测定猪胴体品质。结果表明,PRKAG3主要在骨骼肌中表达,在心脏中有少量表达,而在肝脏和肾中未见表达,在不同品种的骨骼肌中,雅南猪PRKAG3基因的相对表达量高于DLY猪(P=0.078)。相关分析结果表明:骨骼肌中PRKAG3基因的表达量与屠宰率、眼肌面积和背膘厚关系不大,而与肌内脂肪含量成正相关(r=0.832)。展开更多
Eighty-four castrated boars including Laiwu Black （LW） （weight 30-90 kg, n = 6） and Lulai Black （LL） （weight 40-100 kg, n = 6） were used to study the developmental changes of collagen type Ⅲ alpha 1 （Col3al...Eighty-four castrated boars including Laiwu Black （LW） （weight 30-90 kg, n = 6） and Lulai Black （LL） （weight 40-100 kg, n = 6） were used to study the developmental changes of collagen type Ⅲ alpha 1 （Col3al） mRNA expression in the muscle and their association with intramuscular collagen （IMC）. The muscle total RNA was extracted to determine the abundance of Col3al mRNA using relative quantitative RT-PCR with β-actin mRNA as the internal standard. The results indicated that the developmental patterns of muscle Col3al mRNA in LW and LL pigs were similar. The abundance of Col3al mRNA increased with body weight, but decreased a little at 70 kg and 80 kg phases for LW and LL, respectively. On the whole, the expression level of Col3al mRNA in muscle of LW was higher than that of LL （P 〈 0.05）. Correlation analysis showed that the expression of Col3al mRNA in muscle was positively correlated with total and insoluble IMC, but was negatively correlated with IMC solubility for LW pigs （P 〈 0.01） and LL pigs （P 〈 0.05）, respectively. These results suggest that the muscle Col3al gene expression is affected by body weight and genotype and has important effect on IMC content and characteristics.展开更多
文摘PRKAG3是编码一磷酸腺苷激活蛋白激酶(AMP-activated protein kinase,AMPK)γ3亚基的基因,是近年来确定的一个影响猪肉pH值、肉色以及系水力的主效基因,其突变R200Q与过量肌糖原含量有关,被认为是汉普夏猪发生RN-突变的根本原因。本研究以-βactin作为内参,用半定量RT-PCR方法测定雅南猪和杜×长×大三元杂交猪(DLY猪)PRKAG3基因在心脏、肝脏、肾和骨骼肌中表达差异,并测定猪胴体品质。结果表明,PRKAG3主要在骨骼肌中表达,在心脏中有少量表达,而在肝脏和肾中未见表达,在不同品种的骨骼肌中,雅南猪PRKAG3基因的相对表达量高于DLY猪(P=0.078)。相关分析结果表明:骨骼肌中PRKAG3基因的表达量与屠宰率、眼肌面积和背膘厚关系不大,而与肌内脂肪含量成正相关(r=0.832)。
基金This work was supported by the National Animal Breed Resource Preserve Project (No.200014) the Key Project of Shandong Science and Technology Development (No. 20059913) the Sciences Innovation Foundation of Shandong Agricultural University.
文摘Eighty-four castrated boars including Laiwu Black （LW） （weight 30-90 kg, n = 6） and Lulai Black （LL） （weight 40-100 kg, n = 6） were used to study the developmental changes of collagen type Ⅲ alpha 1 （Col3al） mRNA expression in the muscle and their association with intramuscular collagen （IMC）. The muscle total RNA was extracted to determine the abundance of Col3al mRNA using relative quantitative RT-PCR with β-actin mRNA as the internal standard. The results indicated that the developmental patterns of muscle Col3al mRNA in LW and LL pigs were similar. The abundance of Col3al mRNA increased with body weight, but decreased a little at 70 kg and 80 kg phases for LW and LL, respectively. On the whole, the expression level of Col3al mRNA in muscle of LW was higher than that of LL （P 〈 0.05）. Correlation analysis showed that the expression of Col3al mRNA in muscle was positively correlated with total and insoluble IMC, but was negatively correlated with IMC solubility for LW pigs （P 〈 0.01） and LL pigs （P 〈 0.05）, respectively. These results suggest that the muscle Col3al gene expression is affected by body weight and genotype and has important effect on IMC content and characteristics.
