目的:探讨LncRNA PRNCR1(prostate cancer non-coding RNA 1)相关的4个基因多态性位点与胃癌发病风险及病理特征的相关性。方法:研究纳入300例经病理确诊的胃癌患者和300例健康者进行病例对照研究;基因分型采用Mass-array基因分析平台完...目的:探讨LncRNA PRNCR1(prostate cancer non-coding RNA 1)相关的4个基因多态性位点与胃癌发病风险及病理特征的相关性。方法:研究纳入300例经病理确诊的胃癌患者和300例健康者进行病例对照研究;基因分型采用Mass-array基因分析平台完成;采用酶联免疫吸附实验(ELISA)检测幽门螺杆菌(H. pylori)感染状态。结果:LncRNA PRNCR1 rs16901946位点的基因型分布在对照组及病例组中有显著性差异(P=0.018)。与野生型AA基因型相比,rs16901946位点AG基因型(AG vs. AA:校正后OR=1.41,95%CI:1.00～1.98,P=0.048)、GG基因型(GG vs. AA:校正后OR=2.57,95%CI:1.15～5.75,P=0.022)及AG/GG基因型(AG/GG vs. AA:校正后OR=1.51,95%CI:1.09～2.09,P=0.014)的胃癌相对发病风险较高。亚组分析显示,该位点在男性(AG/GG vs. AA:校正后OR=1.82,95%CI:1.23～2.69,P=0.003)、在H. pylori感染人群(AG/GG vs. AA:校正后OR=1.83,95%CI:1.16～2.89,P=0.009)发病风险相对较高。对肿瘤病理特征进行亚组分析显示,该位点与贲门癌的发病风险相关(AG/GG vs. AA:校正后OR=1.58,95%CI:1.10～2.28,P=0.011)、早期胃癌风险相关(AG/GG vs. AA:校正后OR=1.88,95%CI:1.18～2.98,P=0.008)。结论:lncRNA PRNCR1 rs16901946的遗传多态性位点与胃癌发病风险相关,在男性及H. pylori感染阳性人群中的相对风险高。展开更多
目的比较胃癌及其癌旁组织中PRNCR1含量;设计并合成针对PRNCR1基因的siRNA,转染人胃癌细胞BGC-823,观察PRNCR1含量改变对BGC-823细胞增殖活性的影响。方法 Real Time-PCR检测10对胃癌及其癌旁组织中PRNCR1含量;化学法合成针对PRNCR1的si...目的比较胃癌及其癌旁组织中PRNCR1含量;设计并合成针对PRNCR1基因的siRNA,转染人胃癌细胞BGC-823,观察PRNCR1含量改变对BGC-823细胞增殖活性的影响。方法 Real Time-PCR检测10对胃癌及其癌旁组织中PRNCR1含量;化学法合成针对PRNCR1的siRNAs,脂质体法转染至BGC-823细胞,转染分为细胞对照组、转染对照组、错义序列组和siRNA转染组。转染后48 h收集细胞,Real Time-PCR检测转染后细胞内PRNCR1含量变化;CCK-8检测肿瘤细胞对数生长期基因干预对于增殖活性的影响。结果 PRNCR1在胃癌组织中含量明显高于癌旁组织(P<0.05)。与细胞对照组比较,siRNA转染组细胞中PRNCR1含量明显降低,细胞增殖活性明显受到抑制(P<0.01);而转染对照组、错义序列组与细胞对照组比较,PRNCR1含量、细胞增殖活性均无明显差异(P>0.05)。结论 PRNCR1沉默可显著抑制BGC-823细胞增殖活性。展开更多
Prostate cancer non-coding RNA 1(PRNCR1),a lncRNA transcribed from 8q24,is involved in carcinogenesis and development of varies cancer.Several molecular epidemiology studies revealed that the association between PRNCR...Prostate cancer non-coding RNA 1(PRNCR1),a lncRNA transcribed from 8q24,is involved in carcinogenesis and development of varies cancer.Several molecular epidemiology studies revealed that the association between PRNCR1 single-nucleotide polymorphisms(SNPs)and cancer risk,but the results remain controversial.In order to derive a more precise evaluation,we conducted a meta-analysis to summarize all eligible case-control studies to evaluate the association between SNPs in PRNCR1 and the overall cancer susceptibility.A systematic literature search on PubMed and Web of science database was up to January 1st,2017.Finally,a total of six articles involving 2804 cases and 3233 controls samples were included in this meta-analysis.Homozygous,heterozygous,dominant,recessive and allelic models were used to analyze the associations between SNPs in PRNCR1 and cancer risk in the Asian population by using the odds ratio(OR)and 95%confidence interval(95%CIs).Heterogeneity and publication bias were used to measure the robustness of our findings.Significant associations between the PRNCR1 rs16901946 polymorphism and cancer risk were observed(GG vs.AA+AG:OR=1.19,95%CI=1.07-1.32,P=0.002,I2=0.0%)in Asian population.Moreover,subgroup analyses by cancer type indicated that rs1016343 C >T and rs16901946 A >G was associated with an increase prostate cancer risk.In addition,no significant association was detected between rs13252298 A >G and rs7007694 C >T polymorphism and cancer susceptibility.In conclusion,our meta-analysis suggests that PRNCR1 polymorphisms may be associated with cancer susceptibility in Asian populations,particularly in prostate cancer.展开更多
文摘目的:探讨LncRNA PRNCR1(prostate cancer non-coding RNA 1)相关的4个基因多态性位点与胃癌发病风险及病理特征的相关性。方法:研究纳入300例经病理确诊的胃癌患者和300例健康者进行病例对照研究;基因分型采用Mass-array基因分析平台完成;采用酶联免疫吸附实验(ELISA)检测幽门螺杆菌(H. pylori)感染状态。结果:LncRNA PRNCR1 rs16901946位点的基因型分布在对照组及病例组中有显著性差异(P=0.018)。与野生型AA基因型相比,rs16901946位点AG基因型(AG vs. AA:校正后OR=1.41,95%CI:1.00～1.98,P=0.048)、GG基因型(GG vs. AA:校正后OR=2.57,95%CI:1.15～5.75,P=0.022)及AG/GG基因型(AG/GG vs. AA:校正后OR=1.51,95%CI:1.09～2.09,P=0.014)的胃癌相对发病风险较高。亚组分析显示,该位点在男性(AG/GG vs. AA:校正后OR=1.82,95%CI:1.23～2.69,P=0.003)、在H. pylori感染人群(AG/GG vs. AA:校正后OR=1.83,95%CI:1.16～2.89,P=0.009)发病风险相对较高。对肿瘤病理特征进行亚组分析显示,该位点与贲门癌的发病风险相关(AG/GG vs. AA:校正后OR=1.58,95%CI:1.10～2.28,P=0.011)、早期胃癌风险相关(AG/GG vs. AA:校正后OR=1.88,95%CI:1.18～2.98,P=0.008)。结论:lncRNA PRNCR1 rs16901946的遗传多态性位点与胃癌发病风险相关,在男性及H. pylori感染阳性人群中的相对风险高。
文摘Prostate cancer non-coding RNA 1(PRNCR1),a lncRNA transcribed from 8q24,is involved in carcinogenesis and development of varies cancer.Several molecular epidemiology studies revealed that the association between PRNCR1 single-nucleotide polymorphisms(SNPs)and cancer risk,but the results remain controversial.In order to derive a more precise evaluation,we conducted a meta-analysis to summarize all eligible case-control studies to evaluate the association between SNPs in PRNCR1 and the overall cancer susceptibility.A systematic literature search on PubMed and Web of science database was up to January 1st,2017.Finally,a total of six articles involving 2804 cases and 3233 controls samples were included in this meta-analysis.Homozygous,heterozygous,dominant,recessive and allelic models were used to analyze the associations between SNPs in PRNCR1 and cancer risk in the Asian population by using the odds ratio(OR)and 95%confidence interval(95%CIs).Heterogeneity and publication bias were used to measure the robustness of our findings.Significant associations between the PRNCR1 rs16901946 polymorphism and cancer risk were observed(GG vs.AA+AG:OR=1.19,95%CI=1.07-1.32,P=0.002,I2=0.0%)in Asian population.Moreover,subgroup analyses by cancer type indicated that rs1016343 C >T and rs16901946 A >G was associated with an increase prostate cancer risk.In addition,no significant association was detected between rs13252298 A >G and rs7007694 C >T polymorphism and cancer susceptibility.In conclusion,our meta-analysis suggests that PRNCR1 polymorphisms may be associated with cancer susceptibility in Asian populations,particularly in prostate cancer.
