Objective:To study the correlation of PRPS1 and JAB1 expression in the bone marrow tissue of acute leukemia with the apoptosis and invasion of tumor cells.Methods:Patients who were diagnosed with acute leukemia by bon...Objective:To study the correlation of PRPS1 and JAB1 expression in the bone marrow tissue of acute leukemia with the apoptosis and invasion of tumor cells.Methods:Patients who were diagnosed with acute leukemia by bone marrow aspiration biopsy in Edong medical group Huangshi central hospital between February 2015 and December 2017 were selected as the AL group of the research, and patients who received bone marrow aspiration biopsy and were without significant abnormality in Edong medical group Huangshi central hospital during the same period were selected as the control group of the research. RNA was extracted to determine the mRNA expression of PRPS1, JAB1, apoptosis genes and invasion genes, and protein was extracted to determine the protein expression of PRPS1 and JAB1.Results:Both mRNA expression and protein expression of PRPS1 and JAB1 in bone marrow tissues of AL group were significantly higher than those of control group;AuroraA, Bcl-2,β-catenin, MMP2, MMP9, N-cadherin and Msi2 mRNA expression in bone marrow tissues of AL group were significantly higher than those of control group whereas Bax, C/EBP , RUNX3 and TIMP3 mRNA expression were significantly lower than those of control group, and the PRPS1 and JAB1 mRNA expression in bone marrow tissues of AL group were positively correlated with AuroraA, Bcl-2,β-catenin, MMP2, MMP9, N-cadherin and Msi2 mRNA expression, and negatively correlated with Bax, C/EBP , RUNX3 and TIMP3 mRNA expression.Conclusion:The high expression of PRPS1 and JAB1 in the bone marrow tissue of acute leukemia can inhibit cell apoptosis and promote cell invasion.展开更多
We were aiming to delineate, by the utility of the biological data results, in our investigations the link between the purine and pyrimidine metabolism and development of the glioblastoma. We analyzed the sets of the ...We were aiming to delineate, by the utility of the biological data results, in our investigations the link between the purine and pyrimidine metabolism and development of the glioblastoma. We analyzed the sets of the genes, belonging to the purine and pyrimidine metabolism by the utility of GSEA software as well as MSIgnDB application of the GSEA. The GEO database, GEOR2 tools were serving for the visualization of the genes expression profiles of the disease. The Cancer Proteome Atlas as well as the tools of the data sets were also used to collect and analyze the results. We concluded and came to the following consequential results. 1) Neurogenesis and Glioblastoma are sharing some common genes. 2) Purine and pyrimidine metabolism-linked enzymes and genes are responsible for the upregulation of DNA and mRNA synthesis in the settings of the tumor development. 3) EGFR expression responsible genes, mRNA as well as protein is upregulated during the development of the glioblastoma. 4) GMPS genes are more strongly upregulated in the settings of the glioblastoma than ADSL. 5) PRPS1 is strongly synthetized in neurospheres in contrast to the mature tissue during glioblastoma development.展开更多
文摘Objective:To study the correlation of PRPS1 and JAB1 expression in the bone marrow tissue of acute leukemia with the apoptosis and invasion of tumor cells.Methods:Patients who were diagnosed with acute leukemia by bone marrow aspiration biopsy in Edong medical group Huangshi central hospital between February 2015 and December 2017 were selected as the AL group of the research, and patients who received bone marrow aspiration biopsy and were without significant abnormality in Edong medical group Huangshi central hospital during the same period were selected as the control group of the research. RNA was extracted to determine the mRNA expression of PRPS1, JAB1, apoptosis genes and invasion genes, and protein was extracted to determine the protein expression of PRPS1 and JAB1.Results:Both mRNA expression and protein expression of PRPS1 and JAB1 in bone marrow tissues of AL group were significantly higher than those of control group;AuroraA, Bcl-2,β-catenin, MMP2, MMP9, N-cadherin and Msi2 mRNA expression in bone marrow tissues of AL group were significantly higher than those of control group whereas Bax, C/EBP , RUNX3 and TIMP3 mRNA expression were significantly lower than those of control group, and the PRPS1 and JAB1 mRNA expression in bone marrow tissues of AL group were positively correlated with AuroraA, Bcl-2,β-catenin, MMP2, MMP9, N-cadherin and Msi2 mRNA expression, and negatively correlated with Bax, C/EBP , RUNX3 and TIMP3 mRNA expression.Conclusion:The high expression of PRPS1 and JAB1 in the bone marrow tissue of acute leukemia can inhibit cell apoptosis and promote cell invasion.
文摘We were aiming to delineate, by the utility of the biological data results, in our investigations the link between the purine and pyrimidine metabolism and development of the glioblastoma. We analyzed the sets of the genes, belonging to the purine and pyrimidine metabolism by the utility of GSEA software as well as MSIgnDB application of the GSEA. The GEO database, GEOR2 tools were serving for the visualization of the genes expression profiles of the disease. The Cancer Proteome Atlas as well as the tools of the data sets were also used to collect and analyze the results. We concluded and came to the following consequential results. 1) Neurogenesis and Glioblastoma are sharing some common genes. 2) Purine and pyrimidine metabolism-linked enzymes and genes are responsible for the upregulation of DNA and mRNA synthesis in the settings of the tumor development. 3) EGFR expression responsible genes, mRNA as well as protein is upregulated during the development of the glioblastoma. 4) GMPS genes are more strongly upregulated in the settings of the glioblastoma than ADSL. 5) PRPS1 is strongly synthetized in neurospheres in contrast to the mature tissue during glioblastoma development.
