PRUNE2 plays an important role in regulating tumor cell differentiation,proliferation,and invasiveness in neuroblastoma.Our previous study revealed that PRUNE2/OBSCN two-gene relative expression classifer accurately d...PRUNE2 plays an important role in regulating tumor cell differentiation,proliferation,and invasiveness in neuroblastoma.Our previous study revealed that PRUNE2/OBSCN two-gene relative expression classifer accurately differentiated leiomyosarcoma from gastrointestinal stromal tumor.However,the association between PRUNE2 expression and prognosis in leiomyosarcoma is poorly understood.In this study,we evaluated the prognostic role of PRUNE2 in leiomyosarcoma.PRUNE2 expression was detected using immunohistochemistry in 30 formalin-fixed,paraffin-embedded leiomyosarcoma tissues from MD Anderson Cancer Center,and high expression was detected in 36.7%(11/30)of the samples.To validate these results,immunohistochemistry was performed on another cohort of 45 formalin-fixed,paraffinembedded leiomyosarcoma tissues from Tianjin Medical University Cancer Institute&Hospital,and high PRUNE2 protein expression was detected in 37.8%(17/45)of the samples.Moreover,elevated PRUNE2expression was significantly associated with tumor size(P=0.03)and hemorrhage/cyst(P=0.014),and was an independent favorable prognostic factor for overall survival in leiomyosarcoma patients from Tianjin Medical University Cancer Institute&Hospital(P<0.05).These data suggest that increased PRUNE2protein expression may serve as a favorable prognostic marker in human leiomyosarcoma.展开更多
Autism spectrum disorder（ASD） encompasses a complex set of developmental neurological disorders,characterized by de？cits in social communication and excessive repetitive behaviors. In recent years, ASD is increasin...Autism spectrum disorder（ASD） encompasses a complex set of developmental neurological disorders,characterized by de？cits in social communication and excessive repetitive behaviors. In recent years, ASD is increasingly being considered as a disease of the synapse.One main type of genetic aberration leading to ASD is gene duplication, and several mouse models have been generated mimicking these mutations. Here, we studied the effects of MECP2 duplication and human chromosome15q11-13 duplication on synaptic development and neural circuit wiring in the mouse sensory cortices. We showed that mice carrying MECP2 duplication had speci？c defects in spine pruning, while the 15q11-13 duplication mouse model had impaired spine formation. Our results demonstrate that spine pathology varies signi？cantly between autism models and that distinct aspects of neural circuit development may be targeted in different ASD mutations.Our results further underscore the importance of gene dosage in normal development and function of the brain.展开更多
基金partly supported by grants from the National Nature Science Foundation of China (No.81372872 to J.Yang and No.81320108022 to K.Chen)the University Cancer Foundation via the Sister Institution Network Fund (SINF) at the Tianjin Medical University Cancer Institute & Hospital (TMUCIH)+3 种基金Fudan University Shanghai Cancer Center (FUSCC)University of Texas MD Anderson Cancer Center (UTMDACC)Changjiang Scholars and Innovative Research Team in University (PCSIRT) in China (No.IRT1076)National Key Scientific and Technological Project (No.2011ZX09037-001-04) (K.Chen)
文摘PRUNE2 plays an important role in regulating tumor cell differentiation,proliferation,and invasiveness in neuroblastoma.Our previous study revealed that PRUNE2/OBSCN two-gene relative expression classifer accurately differentiated leiomyosarcoma from gastrointestinal stromal tumor.However,the association between PRUNE2 expression and prognosis in leiomyosarcoma is poorly understood.In this study,we evaluated the prognostic role of PRUNE2 in leiomyosarcoma.PRUNE2 expression was detected using immunohistochemistry in 30 formalin-fixed,paraffin-embedded leiomyosarcoma tissues from MD Anderson Cancer Center,and high expression was detected in 36.7%(11/30)of the samples.To validate these results,immunohistochemistry was performed on another cohort of 45 formalin-fixed,paraffinembedded leiomyosarcoma tissues from Tianjin Medical University Cancer Institute&Hospital,and high PRUNE2 protein expression was detected in 37.8%(17/45)of the samples.Moreover,elevated PRUNE2expression was significantly associated with tumor size(P=0.03)and hemorrhage/cyst(P=0.014),and was an independent favorable prognostic factor for overall survival in leiomyosarcoma patients from Tianjin Medical University Cancer Institute&Hospital(P<0.05).These data suggest that increased PRUNE2protein expression may serve as a favorable prognostic marker in human leiomyosarcoma.
基金supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences,China(XDB02010000)the National Natural Science Foundation of China(31530030 and 81371270)the Science and Technology Commission of Shanghai Municipality,China(16XD1404800)
文摘Autism spectrum disorder（ASD） encompasses a complex set of developmental neurological disorders,characterized by de？cits in social communication and excessive repetitive behaviors. In recent years, ASD is increasingly being considered as a disease of the synapse.One main type of genetic aberration leading to ASD is gene duplication, and several mouse models have been generated mimicking these mutations. Here, we studied the effects of MECP2 duplication and human chromosome15q11-13 duplication on synaptic development and neural circuit wiring in the mouse sensory cortices. We showed that mice carrying MECP2 duplication had speci？c defects in spine pruning, while the 15q11-13 duplication mouse model had impaired spine formation. Our results demonstrate that spine pathology varies signi？cantly between autism models and that distinct aspects of neural circuit development may be targeted in different ASD mutations.Our results further underscore the importance of gene dosage in normal development and function of the brain.
