Dental caries is one of the most prevalent and biofilm-associated oral diseases in humans.Streptococcus mutans,with a high ability to form biofilms by adhering to hard surfaces,has been established as an important eti...Dental caries is one of the most prevalent and biofilm-associated oral diseases in humans.Streptococcus mutans,with a high ability to form biofilms by adhering to hard surfaces,has been established as an important etiological agent for dental caries.Therefore,it is crucial to find a way to prevent the formation of cariogenic biofilm.Here,we report an electrospun fibrous membrane that could inhibit the adhesion and biofilm formation of S.mutans.Also,the polystyrene(PS)/polyvinyl pyrrolidone(PVP)electrospun fibrous membrane altered the 3D biofilm architecture and decreased water-insoluble extracellular polysaccharide production.Notably,the anti-adhesion mechanism which laid in Coulomb repulsion between the negatively charged PS/PVP electrospun fibrous membrane and S.mutans was detected by zeta potential.Furthermore,metagenomics sequencing analysis and CCK-8 assay indicated that PS/PVP electrospun fibrous membrane was microbiome-friendly and displayed no influence on the cell viability of human gingival epithelial cells and human oral keratinocytes.Moreover,an in vitro simulation experiment demonstrated that PS/PVP electrospun fibrous membrane could decrease colony-forming unit counts of S.mutans effectively,and PS/PVP electrospun fibrous membrane carrying calcium fluoride displayed better anti-adhesion ability than that of PS/PVP electrospun fibrous membrane alone.Collectively,this research showed that the PS/PVP electrospun fibrous membrane has potential applications in controlling and preventing dental caries.展开更多
基金supported by the grant from National Natural Science Foundation of China(32170046)the Sichuan Science and Technology Program(2022YFH0048)the Technological Innovation and Development Project of Chengdu Bureau of Science and Technology(2021-YF05-01819-SN).
文摘Dental caries is one of the most prevalent and biofilm-associated oral diseases in humans.Streptococcus mutans,with a high ability to form biofilms by adhering to hard surfaces,has been established as an important etiological agent for dental caries.Therefore,it is crucial to find a way to prevent the formation of cariogenic biofilm.Here,we report an electrospun fibrous membrane that could inhibit the adhesion and biofilm formation of S.mutans.Also,the polystyrene(PS)/polyvinyl pyrrolidone(PVP)electrospun fibrous membrane altered the 3D biofilm architecture and decreased water-insoluble extracellular polysaccharide production.Notably,the anti-adhesion mechanism which laid in Coulomb repulsion between the negatively charged PS/PVP electrospun fibrous membrane and S.mutans was detected by zeta potential.Furthermore,metagenomics sequencing analysis and CCK-8 assay indicated that PS/PVP electrospun fibrous membrane was microbiome-friendly and displayed no influence on the cell viability of human gingival epithelial cells and human oral keratinocytes.Moreover,an in vitro simulation experiment demonstrated that PS/PVP electrospun fibrous membrane could decrease colony-forming unit counts of S.mutans effectively,and PS/PVP electrospun fibrous membrane carrying calcium fluoride displayed better anti-adhesion ability than that of PS/PVP electrospun fibrous membrane alone.Collectively,this research showed that the PS/PVP electrospun fibrous membrane has potential applications in controlling and preventing dental caries.
