通过水培试验,以青萍(Lemna minor L)为研究材料,研究不同浓度砷胁迫对青萍生物量、荧光参数、快速光响应和砷富集量的影响。结果显示,随着砷处理浓度增加,青萍生物量、有效量子产量Y(Ⅱ)、潜在光化学效率(F_(v)/F_(o))、最大光化学效率...通过水培试验,以青萍(Lemna minor L)为研究材料,研究不同浓度砷胁迫对青萍生物量、荧光参数、快速光响应和砷富集量的影响。结果显示,随着砷处理浓度增加,青萍生物量、有效量子产量Y(Ⅱ)、潜在光化学效率(F_(v)/F_(o))、最大光化学效率(F_(v)/F_(m))和光化学荧光淬灭系数(q_(P))显著下降(P<0.05),非调节性能量耗散量子产量Y(NO)、调节性能量耗散量子产量Y(NPQ)和砷单位富集量显著上升(P<0.05),非光化学淬灭系数(q_(N))先升后降。电子传递效率(ETR)随着砷处理浓度增大,呈显著下降的趋势;然而当有效辐射强度(PAR)增大时,ETR呈先升后逐渐趋于平稳的趋势。本研究表明,青萍在受到砷胁迫时,可通过调节PSⅡ反应中心进行自我保护,缓解砷毒害;对于低浓度(As^(3+)≤3mg·L^(-1))时,则表现出一定耐受性,且砷浓度越高,青萍对砷单位富集量越大。然而,当为高砷处理浓度时,则产生严重抑制作用。本研究可为青萍修复As^(3+)污染水体提供参考意义。展开更多
Photodamage of some pigments in the isolated photosystem Ⅱ (PS Ⅱ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by means of high performance liquid chromatography. The light induced damag...Photodamage of some pigments in the isolated photosystem Ⅱ (PS Ⅱ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by means of high performance liquid chromatography. The light induced damage of pheophytin a (pheo a) in the complex was observed for the first time. The content of pheo a decreased about 47% by illumination, suggesting only one of the two pheo a molecules in the PSⅡ reaction center complex was damaged. No damage of β carotene was found.展开更多
Photodamage of pheophytin a (pheo a) in the isolated photosystem Ⅱ (PSⅡ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by high performance liquid chromatographic method in detail. The res...Photodamage of pheophytin a (pheo a) in the isolated photosystem Ⅱ (PSⅡ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by high performance liquid chromatographic method in detail. The results showed that: (1) There is one pheo a molecule which is not associated with the primary photochemistry in the PSⅡ reaction center complex. It may be considered that there are two different electron transfer branches in the PSⅡ reaction center just as in the purple bacterium photosynthetic reaction center. (2) The damaged pheo a may be attributed to the one bonding to the D2 protein comparing the D2 subunit in the PSⅡ reaction center with M subunit in the purple bacterium photosynthetic reaction center. (3) A possible arrangement model of redox cofactors in the PSⅡ reaction center was proposed based on our experiment.展开更多
IN recent years,magnetic circular dichroism(MCD)demonstrated its unique advantages inanalysis of metal porphyrin and its interaction with protein.However,there were few re-ports about the application of MCD method in ...IN recent years,magnetic circular dichroism(MCD)demonstrated its unique advantages inanalysis of metal porphyrin and its interaction with protein.However,there were few re-ports about the application of MCD method in photosynthesis field.It is known that most pig-ments in PS Ⅱ-RC are metal porphyrin type.This note reports the MCD spectrum of PS Ⅱ-展开更多
文摘通过水培试验,以青萍(Lemna minor L)为研究材料,研究不同浓度砷胁迫对青萍生物量、荧光参数、快速光响应和砷富集量的影响。结果显示,随着砷处理浓度增加,青萍生物量、有效量子产量Y(Ⅱ)、潜在光化学效率(F_(v)/F_(o))、最大光化学效率(F_(v)/F_(m))和光化学荧光淬灭系数(q_(P))显著下降(P<0.05),非调节性能量耗散量子产量Y(NO)、调节性能量耗散量子产量Y(NPQ)和砷单位富集量显著上升(P<0.05),非光化学淬灭系数(q_(N))先升后降。电子传递效率(ETR)随着砷处理浓度增大,呈显著下降的趋势;然而当有效辐射强度(PAR)增大时,ETR呈先升后逐渐趋于平稳的趋势。本研究表明,青萍在受到砷胁迫时,可通过调节PSⅡ反应中心进行自我保护,缓解砷毒害;对于低浓度(As^(3+)≤3mg·L^(-1))时,则表现出一定耐受性,且砷浓度越高,青萍对砷单位富集量越大。然而,当为高砷处理浓度时,则产生严重抑制作用。本研究可为青萍修复As^(3+)污染水体提供参考意义。
文摘Photodamage of some pigments in the isolated photosystem Ⅱ (PS Ⅱ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by means of high performance liquid chromatography. The light induced damage of pheophytin a (pheo a) in the complex was observed for the first time. The content of pheo a decreased about 47% by illumination, suggesting only one of the two pheo a molecules in the PSⅡ reaction center complex was damaged. No damage of β carotene was found.
文摘Photodamage of pheophytin a (pheo a) in the isolated photosystem Ⅱ (PSⅡ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by high performance liquid chromatographic method in detail. The results showed that: (1) There is one pheo a molecule which is not associated with the primary photochemistry in the PSⅡ reaction center complex. It may be considered that there are two different electron transfer branches in the PSⅡ reaction center just as in the purple bacterium photosynthetic reaction center. (2) The damaged pheo a may be attributed to the one bonding to the D2 protein comparing the D2 subunit in the PSⅡ reaction center with M subunit in the purple bacterium photosynthetic reaction center. (3) A possible arrangement model of redox cofactors in the PSⅡ reaction center was proposed based on our experiment.
文摘IN recent years,magnetic circular dichroism(MCD)demonstrated its unique advantages inanalysis of metal porphyrin and its interaction with protein.However,there were few re-ports about the application of MCD method in photosynthesis field.It is known that most pig-ments in PS Ⅱ-RC are metal porphyrin type.This note reports the MCD spectrum of PS Ⅱ-