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Prevalence and antibiogram study of Salmonella and Staphylococcus aureus in poultry meat 被引量:8
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作者 Ali Akbar Anil Kumar Anal 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2013年第2期163-168,共6页
Objective:To evaluate the presence and antibiogram pattern of Salmonella and Staphylococcus aureus(S.aureus)in retail poultry meat products.Methods:Foodborne pathogens(Salmonella and S.aureus)were isolated from poultr... Objective:To evaluate the presence and antibiogram pattern of Salmonella and Staphylococcus aureus(S.aureus)in retail poultry meat products.Methods:Foodborne pathogens(Salmonella and S.aureus)were isolated from poultry meat and confirmed with the help of biochemical and immunological test.Antibiogram of the isolates were examined by following CLS1 methods.Results:A total number of 209 poultry meat samples were collected and studied in this study.Out of which,5.26%were found contaminated with Salmonella while 18.18%were found contaminated with S.aureus.All the Salmonella and S.aureus isolates were found resistant to at least one antibiotic.About 72.72%of the Salmonella isolates showed resistance to tetracycline,while S.aureus isolates were also found highly resistant to tetracycline equal to 44.73%.One of the Salmonella isolates showed multi-drug resistance to almost six antibiotics out of nine antibiotics used in the study.Multidrug resistant S.aureus isolates were also found in the study.Conclusions:The study confirmed the presence of Salmonella and S.aureus in retail poultry meat.It is a potential threat to consumer health.To reduce the risk of contamination,good hygiene practices are necessary from processing to storage. 展开更多
关键词 ANTIBIOGRAM SALMONELLA STAPHYLOCOCCUS AUREUS Food safety poultry meat
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Safety Assessment and Distribution Characteristics of Heavy Metal Pollutants in Livestock and Poultry Meat from Different Regions of China 被引量:3
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作者 Yajie WANG Heng TAO +3 位作者 Kai CHEN Hu XIA Jinlin HE Guangcan TAO 《Asian Agricultural Research》 2019年第1期76-82,共7页
[Objective] To analyze and compare the heavy metal inspection results of different livestock and poultry meat from different farms,wholesale markets,farmers' markets and supermarkets in different areas of China,in... [Objective] To analyze and compare the heavy metal inspection results of different livestock and poultry meat from different farms,wholesale markets,farmers' markets and supermarkets in different areas of China,in order to evaluate the health risks of livestock and poultry meat.[Method] A total of 10 500 samples of 5 kinds of livestock and poultry meat were collected in North China,Northeast China,East China,Central China,South China,Southwest China and Northwest China during 2015-2017.The samples were analyzed and evaluated with the method of index of food safety( IFS).[Result]the average over-standard rates of Pb,Cd,Cr,Hg and As in samples were 1.10%-2.48%,0.67%-2.48%,1.00%-2.57%,0.81%-1.43% and 0.43%-1.00%,respectively,and the index of food safety was less than 1.The pollution levels of Pb,Cd,Cr,Hg and As in livestock and poultry meat in Southwest China were higher than those in other areas,while the pollution of As in Northeast China was severer than that in other areas.The order of IFS values of different heavy metals in different livestock and poultry meat was the same.[Conclusion] The index of food safety showed that the risk of heavy metal pollution in livestock and poultry meat did not exceed the acceptable limit. 展开更多
关键词 LIVESTOCK and poultry meat Assessment method of index of FOOD SAFETY Heavy metal FOOD SAFETY evaluation
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A Study on the Meat and Bone Meal or Poultry By-product Meal as Protein Substitutes of Fishmeal in Concentrated Diets for Paralichthys olivaceus 被引量:6
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作者 ZHU Wei MAI Kangsen +2 位作者 ZHANG Baigang HU Yangjiang YU Yu 《Journal of Ocean University of China》 SCIE CAS 2006年第1期63-66,共4页
研究被进行在生长表演,幸存和日语的明显的消化的好坏系数(模数转换器) 上在食谱作为鱼粉的代替评估肉和骨头饭和家禽副产品饭(PBM )(MBM ) 的效果挣扎(Paralichthys olivaceus ) 。试验性的食谱包括了 0% , 20% , 40% , 60% 和 80%... 研究被进行在生长表演,幸存和日语的明显的消化的好坏系数(模数转换器) 上在食谱作为鱼粉的代替评估肉和骨头饭和家禽副产品饭(PBM )(MBM ) 的效果挣扎(Paralichthys olivaceus ) 。试验性的食谱包括了 0% , 20% , 40% , 60% 和 80% 全部的鱼粉的 MBM 或 PBMreplacement 分别地。所有食谱是 iso 含氮的并且 isocaloric。 Theresults 证明没有重要差别( P 】 0.05 ),在生长,在处理之中的性能用鱼粉的0%-60% MBM 代替喂了百分比重量获得( WG ,%),身体长度获得( BLG ,%)并且当鱼粉被80% MBM 代替时,模数转换器显著地减少。Theresult 也证明没有重要差别(P 】 0.05 ) 在生长,在所有处理之中的性能和模数转换器与 0% 用食谱喂了 - 有 PBM 的鱼粉的 80% 代替。 展开更多
关键词 鱼粉 生长速度 浸溶性 蛋白质 营养成分
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A Study on the Meat and Bone Meal and Poultry By-product Meal as Protein Substitutes of Fish Meal in Practical Diets for Litopenaeus vannamei Juveniles 被引量:5
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作者 ZHUWei MAIKangsen +2 位作者 ZHANGBaigang WANGFuzhen YUYu 《Journal of Ocean University of China》 SCIE CAS 2004年第2期157-160,共4页
A study was conducted to evaluate the effects of meat and bone meal (MBM) and poultry by-product meal (PBM) as the replacement of fish meal in the diets on the growth performance, survival and apparent digestibility c... A study was conducted to evaluate the effects of meat and bone meal (MBM) and poultry by-product meal (PBM) as the replacement of fish meal in the diets on the growth performance, survival and apparent digestibility coefficients (ADC) of Litopenaeus vannamei. The basal diets were formulated with 22% fish meal and other ingredients which provided about 40% protein and 9% lipid in the diet. The experimental diets included MBM or PBM to replace 0, 20%, 40%, 60% and 80% of total fish meal respectively. All diets were iso-nitrogenous and isocaloric in gross terms. The results showed that there were no significant differences (P>0.05) in growth performance and ADC among the treatments fed with the diets in which 0-60% fish meal had been replaced with MBM, while the percent weight gain (WG, %), body length gain (BLG, %) and ADC significantly decreased when the MBM was up to 80% of the fish meal. There were no significant differences (P>0.05) in growth performance and ADC among all the treatments fed with the diets in which 0-80% fish meal had been replaced with PBM. 展开更多
关键词 海溶液 鱼类饲料替代品 生长演示 消化系数 蛋白质 家禽工业饲料
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Meat quality in “in door” and “out door” production systems of poultry and swine
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作者 Wagner A. G. Araújo Luiz F. T. Albino +2 位作者 Nilva K. Sakomura Pedro V. R. Paulino Anastacia M. Campos 《Open Journal of Animal Sciences》 2011年第3期75-88,共14页
The meat quality can be influenced by many interacting factors before and after the slaughter. Currently more sustainable production systems are targeted in general, whether or not they have any effect on meat quality... The meat quality can be influenced by many interacting factors before and after the slaughter. Currently more sustainable production systems are targeted in general, whether or not they have any effect on meat quality. The sustainability is a condition of agroecology and necessarily im- plies on the animal and plant association and succession. A condition for sustainability is to minimize or even eliminate the use of inputs from processes of chemical synthesis. In the case of pigs and poultry, this is feasible by adopting production systems that allows nutria- ents recycle directly on the soil at levels that do not involve pollution. Although we have the understanding that the general principles of sustainability to be observed are universal, the solution is not simple. For each situation a vi- able alternative must be sought, depending on the social, economic, ecological and cultural realities. In tropical and subtropical climates the production of pigs and poultry outdoors can be an appropriate option. This also leads to nutria- ents recycle and promotes a better energy bal- ance of the system. Among the alternatives that can be taken to introduce differentiating factors in meat production as food is the type of pro- duction system, due to its direct impact on the meat quality. These systems have a direct in- fluence through the consumed food, by the conditions of animal wellbeing, physical activity and the environment provided. The performance and meat quality depend on the interaction of genotypes, rearing conditions, pre-slaughter handling and processing of the meat and the carcass. The influence of the rearing system on the animal performance, on the carcass and finally on the meat is the result of the interactive effects among facilities, feeding level and ge-notype used in the production systems. The production of poultry and pigs more extensively tend to get a final product with its own or- ga-noleptic characteristics, changing the meat de-fault color and content, place of fat deposi- tion and the fatty acid profile deposited on the carcass. 展开更多
关键词 ALTERNATIVE Systems meat Quality poultry SWINE
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Pre-column Derivatization-High Performance Liquid Chromatography for the Detection of Monensin in Livestock and Poultry Meat
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作者 Jiao WANG Xiujuan WANG +2 位作者 Lingtong HU Guixia YANG Dandan HU 《Agricultural Biotechnology》 CAS 2021年第2期93-94,共2页
[Objectives]A method for the detection of monensin in poultry and livestock meat by pre-column derivatization-high performance liquid chromatography was established.[Methods]The sample was extracted with chloroform,de... [Objectives]A method for the detection of monensin in poultry and livestock meat by pre-column derivatization-high performance liquid chromatography was established.[Methods]The sample was extracted with chloroform,derivatized with trichloroacetic acid and 2,4-dinitrophenylhydrazine,and centrifuged to obtain a purified solution.A C18 chromatographic column(4.6 mm×150 mm,5μm)was used for separation with(1.5%)acetic acid water∶methanol(volume ratio)=1∶9 as the mobile phase using a DAD detector for detection,and the external standard method was adopted for peak area quantification.[Results]Monensin had good linearity in the concentration range of 5.00-200 mg/L,with the linear correlation coefficient r 2>0.999;the detection limit was 5.00 mg/kg;the relative standard deviation was smaller than 10%;and the recoveries of standard addition experiment were in the range of 75%-110%.[Conclusions]The method has the advantages of simple pretreatment operation,good derivatization effect and fast detection speed,and is suitable for detecting monensin in poultry and livestock meat. 展开更多
关键词 Pre-column derivatization-high performance liquid chromatography Livestock and poultry meat MONENSIN
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禽肉预制菜产业的发展现状及其质量安全的影响因素与提升策略
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作者 黄胜海 唐修君 +3 位作者 贾晓旭 马丽娜 刘茵茵 高玉时 《中国农业科技导报》 CAS CSCD 北大核心 2024年第6期133-140,共8页
禽肉预制菜对提高人们生活水平、乡村振兴、餐饮业标准化、家禽业高质量发展具有重要作用。然而,禽肉预制菜的生产经营与消费过程中存在诸多影响质量安全的不利因素。对家禽养殖和屠宰,禽肉预制菜的加工、包装、贮藏与运输、销售与消费... 禽肉预制菜对提高人们生活水平、乡村振兴、餐饮业标准化、家禽业高质量发展具有重要作用。然而,禽肉预制菜的生产经营与消费过程中存在诸多影响质量安全的不利因素。对家禽养殖和屠宰,禽肉预制菜的加工、包装、贮藏与运输、销售与消费等环节中影响质量安全的因素进行分析;从家禽生态养殖环境的建立与投入品管理、家禽屠宰的场地建设与生产管理、禽肉预制菜加工工艺与绿色标签制度的实施、包装材料的选择与包装技术的应用、贮藏与运输中科学管理体系的建立与信息技术的应用、销售管理制度的健全与对消费者的科学指导等方面提出相应的优化策略,以促进禽肉预制菜质量安全的提升和产业发展,为乡村振兴、“三农”问题的解决、家禽业和餐饮业的发展、人民群众对美好生活的需要和生态文明建设提供理论基础和模式借鉴。 展开更多
关键词 禽肉预制菜 质量安全 产业发展 提升策略
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家禽肉品质评价指标及其影响因素研究进展
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作者 陈安琪 杨春彦 +6 位作者 董丙强 周婷婷 陈世豪 白皓 江勇 常国斌 王志秀 《东北农业大学学报》 CAS CSCD 北大核心 2024年第5期90-100,共11页
近年来,随着人口持续增长,人们生活水平不断提高,禽肉需求量不断增长,消费者对其品质要求也越来越高。目前,禽肉市场存在许多问题,食品安全问题日益增加。从评价肉品质的重要指标(肉色、pH、系水力、剪切力、水分含量、蛋白质含量、脂... 近年来,随着人口持续增长,人们生活水平不断提高,禽肉需求量不断增长,消费者对其品质要求也越来越高。目前,禽肉市场存在许多问题,食品安全问题日益增加。从评价肉品质的重要指标(肉色、pH、系水力、剪切力、水分含量、蛋白质含量、脂肪含量及风味物质中存在的氨基酸、脂肪酸和肌苷酸)及影响家禽肉品质的品种、性别、日龄以及饲养管理和环境等方面多角度分析养殖过程中影响肉质优劣的重要影响因素,以期为生产优质禽肉的饲养管理及遗传育种提供参考。 展开更多
关键词 家禽 肉品质 评价指标 影响因素
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家禽骨骼肌肌纤维类型影响因素及其转化的研究进展
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作者 谢兵红 单艳菊 +5 位作者 范晨宇 薛夫光 吴红翔 巨晓军 束婧婷 刘一帆 《中国畜牧兽医》 CAS CSCD 北大核心 2024年第4期1561-1572,共12页
肌纤维是构成肌肉组织的基本单位。肌肉的肌纤维类型组成与肉品质密切相关,并可在机体生长发育、营养和饲养环境等外界刺激下发生变化。近年来,由于消费者和育种学家对肉品质的关注,家禽肌纤维类型及其转换规律已成为本领域的研究热点... 肌纤维是构成肌肉组织的基本单位。肌肉的肌纤维类型组成与肉品质密切相关,并可在机体生长发育、营养和饲养环境等外界刺激下发生变化。近年来,由于消费者和育种学家对肉品质的关注,家禽肌纤维类型及其转换规律已成为本领域的研究热点之一。随着组学研究技术的发展,转录组学、蛋白组学等技术已广泛应用于家禽肌纤维类型调控机理的研究,极大地推动了相关研究的进展。充分了解目前有关肌纤维类型转化的研究,是后续开展相关肉品质与调控机制研究的重要理论基础。在此背景下,作者介绍了肌纤维的结构组成及不同类型的分类方法,简述了肌纤维类型与家禽肌肉品质特征相关性状如肉色、pH、系水力和嫩度之间的关系,并总结了生长、品种、环境和饲养方式等因素对肌纤维类型的影响,重点讨论了现有的分子调控研究进展,旨在为家禽肌纤维类型转换机理的研究提供参考。最后,对未来优质肉鸡的育种方向提出了展望,希望随着现代分子生物技术的发展,单细胞/空间组学技术、基于CRISPR/Cas9系统的基因编辑技术等前沿技术能在肌纤维类型研究领域广泛运用,肌纤维类型转换的机制研究将不断完善,并建立家禽肉质性状的分子选育方法。 展开更多
关键词 家禽 肉品质 肌纤维类型 肌纤维类型转换
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谷氨酸的生理功能及其在畜禽生产中的应用研究进展
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作者 黄钰琴 王力仪 单体中 《动物营养学报》 CAS CSCD 北大核心 2024年第2期757-768,共12页
氨基酸是蛋白质和小肽的基本组成单位,在动物生长、发育、免疫、繁殖及维持机体稳态等方面发挥着重要的作用。谷氨酸(Glu)是动物组织含量丰富的非必需氨基酸之一,在介导兴奋性神经传递、维持肠道功能完整性、调控机体能量稳态等过程中... 氨基酸是蛋白质和小肽的基本组成单位,在动物生长、发育、免疫、繁殖及维持机体稳态等方面发挥着重要的作用。谷氨酸(Glu)是动物组织含量丰富的非必需氨基酸之一,在介导兴奋性神经传递、维持肠道功能完整性、调控机体能量稳态等过程中发挥重要作用。本文主要综述了Glu的理化性质、生理功能及其在畜禽生产上的应用研究进展,以期为今后Glu在畜禽生产上的开发利用提供参考。 展开更多
关键词 谷氨酸 氨基酸营养 畜禽 生长发育 肉品质
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胶体金免疫层析法同步快速测定畜禽肉中3种兽药残留
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作者 郝秋艳 周嘉明 +2 位作者 智军海 贾先春 王玮 《食品与发酵工业》 CAS CSCD 北大核心 2024年第11期284-293,共10页
该文旨在建立一种胶体金免疫层析技术同步测定猪肉和鸡肉组织中呋喃唑酮代谢物、呋喃西林代谢物、氯霉素药物残留的方法。动物肌肉组织中呋喃唑酮代谢物、呋喃西林代谢物、氯霉素经酸化、衍生化及乙酸乙酯提取,并经含吐温-20的Tris-HCl... 该文旨在建立一种胶体金免疫层析技术同步测定猪肉和鸡肉组织中呋喃唑酮代谢物、呋喃西林代谢物、氯霉素药物残留的方法。动物肌肉组织中呋喃唑酮代谢物、呋喃西林代谢物、氯霉素经酸化、衍生化及乙酸乙酯提取,并经含吐温-20的Tris-HCl缓冲液稀释后,与胶体金标记的单克隆抗体结合,抑制抗体和硝酸纤维素膜检测线(T线)上的抗原结合,从而导致T线颜色变化。通过比较T线和质控线(C线)颜色深浅,对试样中多兽药残留进行定性判定。试验优化了pH值、抗体添加量、抗原包被量、吐温-20添加量、衍生化试剂浓度和冻干保护剂种类等关键因素,并对检测卡的灵敏度、特异性和稳定性进行检测。结果显示,所建立的免疫层析方法可在35 min内检出猪肉和鸡肉中呋喃西林代谢物、呋喃唑酮代谢物和氯霉素药物,检出限分别为0.5、0.5、0.1μg/kg,灵敏度≥98%,假阳性率≤2%,假阴性率≤2%,稳定性可达到1年,且与现有标准方法检测结果一致。该方法操作简便、特异性强、灵敏度和准确度高,检测时间短且稳定性好,可用于动物肌肉组织中多兽药残留的高通量快速检测。 展开更多
关键词 多兽药残留 畜禽肉 同步快速测定 胶体金免疫层析法(GICA)
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QuEChERS-UPLC-MS/MS同时测定畜禽肉中23种驱虫类药物残留量
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作者 张彤 徐宜宏 +5 位作者 付海滨 刘俊 史喜菊 徐蕾蕊 康明芹 张敏 《饲料工业》 CAS 北大核心 2024年第3期120-126,共7页
研究旨在建立同时检测畜禽肉中多种驱虫类药物的分析方法,通过优化前处理方法和仪器分析条件,建立了分散固相萃取法-超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定畜禽肉中23种驱虫类药物残留的分析方法,目标物以乙腈提取,分散固相萃... 研究旨在建立同时检测畜禽肉中多种驱虫类药物的分析方法,通过优化前处理方法和仪器分析条件,建立了分散固相萃取法-超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定畜禽肉中23种驱虫类药物残留的分析方法,目标物以乙腈提取,分散固相萃取法(QuEChERS)净化,用乙腈+0.1%甲酸水作为流动相、UPLC-MS/MS确证检测、外标法定量。结果显示:所建立的方法定量限为0.2~1.0μg/kg,回收率为71.4%~107.5%,相对标准偏差为0.9%~8.8%。表明该方法定量限低,稳定性好,适用于畜禽肉中23种驱虫类药物残留的同时检测。 展开更多
关键词 驱虫药 超高效液相色谱-串联质谱 畜禽肉 药物残留 分散固相萃取法
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内蒙古自治区生禽畜肉沙门氏菌、单核细胞增生李斯特菌和弯曲菌的污染监测分析
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作者 李茜茜 乌伊罕 曲琳 《包头医学院学报》 CAS 2024年第4期67-71,86,共6页
目的:了解内蒙古自治区生禽畜肉中沙门氏菌、单核细胞增生李斯特菌和弯曲菌污染状况,为防治食源性疾病提供基础资料,为相关监督部门提供指导意见。方法:2016~2020年,从内蒙古自治区12个盟市采集生禽肉和牲畜肉共3171份样品。依据《食品... 目的:了解内蒙古自治区生禽畜肉中沙门氏菌、单核细胞增生李斯特菌和弯曲菌污染状况,为防治食源性疾病提供基础资料,为相关监督部门提供指导意见。方法:2016~2020年,从内蒙古自治区12个盟市采集生禽肉和牲畜肉共3171份样品。依据《食品安全国家标准》(GB 4789-2016、GB 4789-2014)和食源性疾病监测工作手册要求进行致病菌的监测。结果:2016~2020年,在3171份样品中共检出阳性致病菌346株,总检出率为10.91%(346/3171),其中沙门氏菌、单核细胞增生李斯特菌和弯曲菌的总检出率依次为8.56%(85/993)、5.62%(50/889)和16.37%(211/1289);散装样品致病菌污染率高于预包装;冷冻肉中单核细胞增生李斯特菌的检出率最高,为17.53%(27/154);沙门氏菌在网店和便利店/零售店的检出率较高,分别为22.22%(4/18)和14.57%(22/151);弯曲菌在养殖环节的检出率最高,为40.55%(103/254),屠宰环节中样品均未检出。结论:2016~2020年内蒙古自治区生禽畜肉中弯曲菌污染最高,单核细胞增生李斯特氏菌污染率较低。为减少食源性疾病的发生率,应加强我区生禽畜肉在不同环节的监督管理,保障国民的食品安全与健康。 展开更多
关键词 生禽畜肉 沙门氏菌 单核细胞增生李斯特菌 弯曲菌
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高效液相色谱-电感耦合等离子体质谱法测定畜禽肉中5种砷形态的含量
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作者 何兵兵 乔晴 +1 位作者 李辰 王向军 《理化检验(化学分册)》 CAS CSCD 北大核心 2024年第6期593-597,共5页
提出了高效液相色谱-电感耦合等离子体质谱法(HPLC-ICP-MS)测定畜禽肉中三价砷[As(Ⅲ)]、五价砷[As(Ⅴ)]、二甲基砷(DMA)、一甲基砷(MMA)、砷甜菜碱(AsB)等5种砷形态含量的方法。取样品1.000 g,加入10 mL含1%(体积分数)硝酸的10%(体积分... 提出了高效液相色谱-电感耦合等离子体质谱法(HPLC-ICP-MS)测定畜禽肉中三价砷[As(Ⅲ)]、五价砷[As(Ⅴ)]、二甲基砷(DMA)、一甲基砷(MMA)、砷甜菜碱(AsB)等5种砷形态含量的方法。取样品1.000 g,加入10 mL含1%(体积分数)硝酸的10%(体积分数)甲醇溶液(提取剂),于60℃超声提取2.0 h。冷却,离心,分取5 mL上清液,加入5 mL正己烷,振摇1 min,离心10 min,弃去上层正己烷相,再加入5 mL正己烷重复上述步骤1次。吸取下层清液,过0.45μm有机滤膜。以Agilent ZORBAX SB-Aq色谱柱(250 mm×4.6 mm,5μm)为分离柱,以含5 mmol·L^(-1)己烷磺酸钠的20 mmol·L^(-1)柠檬酸溶液(pH 4.3)为流动相进行等度洗脱滤液中的目标物。结果表明:5种砷形态标准曲线的线性范围均为2~50μg·L^(-1),检出限(3S/N)为0.1~0.2μg·L^(-1);对实际样品进行3个浓度水平的加标回收试验,5种砷形态的回收率为81.5%~101%,测定值的相对标准偏差(n=6)为1.3%~3.6%。方法用于10份实际样品分析,其中As(Ⅲ)、DMA和AsB的检出率较高,肝脏、肾脏中的砷形态检出量高于肌肉中的,但均远低于GB 2762—2017对肉及肉制品中总砷的限量(0.5 mg·kg^(-1))要求。 展开更多
关键词 畜禽肉 砷形态 超声提取 高效液相色谱-电感耦合等离子体质谱法 测定
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Captiva EMR-Lipid技术结合液相色谱-串联质谱法测定畜禽肉及内脏中4种抗生素残留
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作者 申雷 柳溪 +1 位作者 羊银 史长生 《食品安全质量检测学报》 CAS 2024年第11期220-229,共10页
目的建立液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)测定畜禽肉及内脏中4种抗生素(新生霉素、莫匹罗星、丰加霉素、茴香霉素)残留的检测方法。方法样品经2%甲酸乙腈溶液提取和增强型脂质去除(Capti... 目的建立液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)测定畜禽肉及内脏中4种抗生素(新生霉素、莫匹罗星、丰加霉素、茴香霉素)残留的检测方法。方法样品经2%甲酸乙腈溶液提取和增强型脂质去除(Captiva EMR-Lipid)固相萃取柱净化,采用Waters T3色谱柱分离目标物,流动相以乙腈和含0.1%甲酸10 mmol/L甲酸铵水溶液梯度洗脱,并采用电喷雾离子源的正离子扫描模式,空白基质匹配外标法定量。结果新生霉素、莫匹罗星、丰加霉素、茴香霉素在2.5~100.0 ng/mL范围内线性关系良好,相关系数(r 2)均大于0.9950。方法的检出限为1.0μg/kg,定量限为3.0μg/kg。当加标回收实验的添加量为3.0~30.0μg/kg时,新生霉素、莫匹罗星、丰加霉素、茴香霉素加标回收率为76.3%~115.1%,相对标准偏差均小于8%(n=6),在猪肉基质中日内精密度和日间精密度均小于9%(n=5)。结论本研究建立的检测方法,样品前处理快速简便,检测灵敏度高,适用于市售畜禽肉及内脏中抗生素的风险筛查。 展开更多
关键词 液相色谱-串联质谱法 抗生素 畜禽肉及内脏 固相萃取 兽药残留
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The Effects of Residual Blood of Carcasses on Poultry Technological Quality
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作者 Bourbab Mohamed Idaomar Mohamed 《Food and Nutrition Sciences》 2012年第10期1382-1386,共5页
The objective of this work is to show the effect of residual blood on the quality of chicken meat, work based on the hypothesis that increasing the concentration of residual blood would explain the observed quality de... The objective of this work is to show the effect of residual blood on the quality of chicken meat, work based on the hypothesis that increasing the concentration of residual blood would explain the observed quality degradation by several authors. Lots of chickens are killed with two different types of slaughter: Slaughter without stunning for a maximum flow of blood or bleeding perfect PS, shooting seals slight lead to keep almost all blood from the carcass or bleeding IS flawed. To prove the residual blood factors that are responsible for high pHu level and high values of (L* A* B*) in poultry, this study compares the pHu level and values of (L* A* B*) in poultry both perfectly and imperfectly bled. Samples from imperfectly bled carcasses show a high value average number of A* = 12.68 and B* = 16.85;and L* = 54.09 VS the average number of A* = 8.50 and B* = 14.43 and L* = 50.27. At 3℃ storage temperature, the average pHu of perfectly bled poultry was (5.7) which was significantly (P < 0.01) lower than the average pHu of imperfectly bled poultry (6.08). At 7℃ storage temperature, the average pHu of perfectly bled poultry was (6.07) which was significantly (P < 0.05) lower than the average pHu of imperfectly bled one (6.27). We found out the effect of season on the results of pHu measures, so we conducted aanalysis of pHu in summer and winter. Residual blood of the poultry is responsible for high values of (L* A* B*) and high values of pHu therefore the deterioration of the poultry Colour. 展开更多
关键词 meat QUALITY RESIDUAL BLOOD poultry meat Colour meat pHu Perfectly Bled (PS) Imperfectly Bled (IB)
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江苏市售禽肉中弯曲杆菌的检测及耐药性分析 被引量:1
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作者 张小燕 周倩 +4 位作者 唐梦君 马丽娜 丁红梅 陆俊贤 高玉时 《扬州大学学报(农业与生命科学版)》 CAS 北大核心 2023年第6期84-90,共7页
为了解江苏市售禽肉中弯曲杆菌的污染及耐药情况,从江苏省10个市区农贸市场及超市采集252份新鲜鸡肉、鸭肉、鸽肉样品进行弯曲杆菌的分离鉴定,采用纸片扩散法测定分离菌株对8类10种抗菌药物的耐药性,采用PCR及测序技术检测菌株携带重要... 为了解江苏市售禽肉中弯曲杆菌的污染及耐药情况,从江苏省10个市区农贸市场及超市采集252份新鲜鸡肉、鸭肉、鸽肉样品进行弯曲杆菌的分离鉴定,采用纸片扩散法测定分离菌株对8类10种抗菌药物的耐药性,采用PCR及测序技术检测菌株携带重要耐药基因的情况。结果表明:共分离到34株空肠弯曲杆菌和66株结肠弯曲杆菌,阳性率分别为13.5%和26.2%。鸡肉、鸭肉、鸽肉样品中弯曲杆菌阳性率分别为41.4%、29.6%和38.2%。耐药性检测结果显示,弯曲杆菌对四环素、红霉素、阿奇霉素、妥布霉素、氨苄西林和庆大霉素耐药率分别为60.0%、54.0%、54.0%、52.0%、50.0%和41.0%,对氟苯尼考和磷霉素耐药率分别为13.0%和2.0%,结肠弯曲杆菌的多重耐药率(84.8%)显著高于空肠弯曲杆菌(26.5%)。分离菌株对10种抗生素共产生31种耐药谱,空肠弯曲杆菌和结肠弯曲杆菌的优势耐药谱分别为AMP-CN-TOB-CIP-TE和TOB-E-AZM-SXT-TE。aadE-sat4-aphA-3及RE-cmeABC检出率分别为26.0%和20.0%,其与弯曲杆菌多重耐药表型符合率分别为90.9%和83.3%。这一研究提示,江苏市售禽肉中存在多重耐药弯曲杆菌及重要耐药基因的流行,对食品安全有潜在的威胁,应进一步加强禽肉中弯曲杆菌的监测和防控。 展开更多
关键词 禽肉 弯曲杆菌 药物敏感性 耐药基因
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Effects of Moringa (Moringa oleifera Lam.) Leaf Meal on Performance, Carcass, Organs, Eggs and Meat of Japanese Quails
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作者 Sati NM Idahor KO +3 位作者 Emennaa PE Haliru H Nwamo AC Markus KD 《Journal of Zoological Research》 2021年第1期15-22,共8页
There are several reports on the utilisation of Moringa oleifera in poultry diets due to its essential bioactive compounds yet,little is known about its influence on Japanese quail eggs and meat qualities.Hence,the ne... There are several reports on the utilisation of Moringa oleifera in poultry diets due to its essential bioactive compounds yet,little is known about its influence on Japanese quail eggs and meat qualities.Hence,the need to examine performance,eggs and meat qualities of Japanese quail hens fed M.oleifera leaf.To achieve this,240 Japanese quail chicks were allocated to three dietary treatments:D1:control,0.0%(without M.oleifera leaf meal),D2:(0.5%M.oleifera leaf meal)and D3:(1%M.oleifera leaf meal).Data on performance,carcass,organs,eggs and meat qualities were collected and subjected to ANOVA at 0.05.Results revealed that feed consumption was lowest(2,701g)in D1 and highest(2,800g)in D2,carcass weight varied from 100-100.67g,thigh weight(12.66-13.58g)and breast weight was highest(40.41g)in D3.Liver weight was lowest(3.25g)in D1,kidney was largest(0.91g)in D3 whereas,the heart,gizzard and spleen weights ranged from 1.00-1.16g,3.08-3.50g and 0.04-0.08g,respectively.In the eggs,crude protein(10.94%),crude fat(6.71%),ash(1.36%),high-density lipoprotein(96.12mg/100g)and low-density lipoprotein(120.67mg/100g)were highest in D1.Total cholesterol(364.08mg/100g)and triglycerides(147.27mg/100g)were least in D1 and the caloric value varied from 1.46-1.47kcal/g.In the meat,crude protein(17.14%)and energy value(1.96kcal/g)were best in D2 but,crude fat(12.62%),ash(2.85%)and carbohydrates(1.31%)were superior in D3.In both eggs and meat,no crude fibre(0.0%)was detected.In any case,all the parameter values were within the normal ranges given in healthy Japanese quails at similar age.Consequently,addition of M.oleifera leaf meal at 1.0%to Japanese quail diets might not depress performance,affect carcass quality,cause organs dysfunctions but may improve nutritional quality of the eggs and meat. 展开更多
关键词 Moringa oleifera poultry nutrition Quail eggs and meat
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超声波辅助煮制对畜禽肉制品品质影响研究进展 被引量:4
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作者 刘薇 赵赵 +3 位作者 朱文政 沙文轩 章海风 周晓燕 《食品工业科技》 CAS 北大核心 2023年第7期419-427,共9页
超声波对畜禽肉制品加工主要通过超声波产生的空化等作用,使肉质结构、营养成分、风味物质等发生改变,从而对其品质产生影响。超声辅助煮制作为新型煮制方式,与传统煮制方式相比,其高频率、高热量的优势可快速提高食物煮制效果,短时间... 超声波对畜禽肉制品加工主要通过超声波产生的空化等作用,使肉质结构、营养成分、风味物质等发生改变,从而对其品质产生影响。超声辅助煮制作为新型煮制方式,与传统煮制方式相比,其高频率、高热量的优势可快速提高食物煮制效果,短时间内达到并优于传统长时煮制的效果。本文立足于超声辅助煮制,结合国内外研究进展,论述超声辅助煮制对畜禽肉制品品质的影响,为畜禽肉制品后续工业化生产提供一定的理论基础,在未来食品生产加工新方向中起到积极的引导作用。 展开更多
关键词 超声辅助煮制 畜禽肉制品 质构 嫩度 风味
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影响家禽肉质的因素及研究现状 被引量:3
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作者 赫云桐 周长海 +5 位作者 梁芳芳 段楚枫 孙睿鸿 李玉梅 张晶 刁继辙 《饲料工业》 CAS 北大核心 2023年第5期20-24,共5页
肉品质的评价指标主要包括pH、肉色、嫩度、系水力以及风味。近年来禽类肉质问题日益突出,其品质直接影响消费者的感官印象,从而影响禽肉的经济效益,因此探究禽肉品质影响因素对于生产具有指导意义。文章综述了禽肉评价指标的影响因素,... 肉品质的评价指标主要包括pH、肉色、嫩度、系水力以及风味。近年来禽类肉质问题日益突出,其品质直接影响消费者的感官印象,从而影响禽肉的经济效益,因此探究禽肉品质影响因素对于生产具有指导意义。文章综述了禽肉评价指标的影响因素,以期为后续研究提供参考。 展开更多
关键词 家禽 肉品质 氨基酸 脂肪酸 微量元素 维生素 肌苷酸 环境温度
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