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嗜麦芽假单胞菌质粒pSH1的限制图谱及km^r标记的克隆
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作者 黄玉屏 沈萍 彭珍荣 《武汉大学学报(自然科学版)》 CSCD 1994年第2期115-121,共7页
对嗜麦芽假单胞菌P2菌株(PseudomonasmaltoohiliaP2)的质粒pSH1进行了限制酶切分析,确定了BglⅡ、EcoRⅠ、PstⅠ、XbaⅠ、BamHⅠ、BglⅠ、及PvuⅡ共7种限制性内切酶在pSH... 对嗜麦芽假单胞菌P2菌株(PseudomonasmaltoohiliaP2)的质粒pSH1进行了限制酶切分析,确定了BglⅡ、EcoRⅠ、PstⅠ、XbaⅠ、BamHⅠ、BglⅠ、及PvuⅡ共7种限制性内切酶在pSH1、质粒上的切割位点,前4种酶均为单一切点;后3种依次为2、7、5个切点.通过双酶切和部分酶切的方法绘制了pSH1质粒的限制酶切图谱.将pGP1-2质粒上的卡那霉素抗性基因(kmr)片段插入pSH1,获得了重组质粒pSH2.pSH2是由pGP1-2质粒上大小为2.90kb的DNA片段(含kmr)和ghH1经BamHⅠ-PstⅠ双酶切产生5.70kb大片段组成,它能够重新转化到喀麦芽假单胞菌受体(kmr)中,其kmr标记能够得到稳定的表达. 展开更多
关键词 pshl质粒 嗜麦芽 假单胞菌
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Otolithic organ function in patients with profound sensorineural hearing loss 被引量:1
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作者 Yujuan Zhou Yongzhen Wu Jing Wang 《Journal of Otology》 CSCD 2016年第2期73-77,共5页
Profound sensorineural hearing loss(PSHL) is not uncommonly encountered in otology. In clinics, there is a high incidence of otolithic damage in patients with PSHL, but relevant reports are few. Sharing a continuous m... Profound sensorineural hearing loss(PSHL) is not uncommonly encountered in otology. In clinics, there is a high incidence of otolithic damage in patients with PSHL, but relevant reports are few. Sharing a continuous membranous structure and similar receptor cell ultrastructures,the cochlea and vestibule may be susceptible to the same harmful factors. Disorders of the inner ear may result in a variety of manifestations,including vertigo, spatial disorientation, blurred vision, impaired articulation, and hearing impairment. Considering the diversity of clinical symptoms associated with PSHL with otolithic dysfunction, it may be frequently misdiagnosed, and objective means of testing the function of otolithic organs should be recommended for hearing-impaired patients. Vestibular-evoked myogenic potentials(VEMPs) via air-conducted sound are of great importance for the diagnosis of otolithic function. Hearing devices such as cochlear implants are commonly accepted treatments for PSHL, and early identification and treatment of vestibular disorders may increase the success rate of cochlear implantation.Therefore, it is necessary to increase awareness of otolithic functional states in patients with PSHL. 展开更多
关键词 PROFOUND SENSORINEURAL hearing loss pshl Otolithic organs Vestibular-evoked MYOGENIC potential VEMP
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