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Component spectroscopic properties of light-harvesting complexes with DFT calculations
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作者 SHYAM BADU SANJAY PRABHAKAR RODERICK MELNIK 《BIOCELL》 SCIE 2020年第3期279-291,共13页
Photosynthesis is a fundamental process in biosciences and biotechnology that influences profoundly the research in other disciplines.In this paper,we focus on the characterization of fundamental components,present in... Photosynthesis is a fundamental process in biosciences and biotechnology that influences profoundly the research in other disciplines.In this paper,we focus on the characterization of fundamental components,present in pigment-protein complexes,in terms of their spectroscopic properties such as infrared spectra,nuclear magnetic resonance,as well as nuclear quadrupole resonance,which are of critical importance for many applications.Such components include chlorophylls and bacteriochlorophylls.Based on the density functional theory method,we calculate the main spectroscopic characteristics of these components for the Fenna-Matthews-Olson light-harvesting complex,analyze them and compare them with available experimental results.Future outlook is discussed in the context of current and potential applications of the presented results. 展开更多
关键词 PHOTOSYNTHESIS BACTERIOCHLOROPHYLLS Light HARVESTING pigment-protein complexes Density functional theory complex networks Photocatalysis BIOSENSING and bioplasmonics
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复杂因素影响下的福州市地面沉降时空演化分析 被引量:8
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作者 兰恒星 孟云闪 仉义星 《工程地质学报》 CSCD 北大核心 2019年第6期1350-1361,共12页
地面沉降是福州市的主要地质灾害之一,自20世纪中期以来就有监测资料显示福州市存在地面沉降问题。本文基于永久散射体雷达干涉测量技术(IPTA),处理了福州市2008~2014年间多时相、高分辨率Terra SAR-X数据,对福州市6年时间的地面沉降进... 地面沉降是福州市的主要地质灾害之一,自20世纪中期以来就有监测资料显示福州市存在地面沉降问题。本文基于永久散射体雷达干涉测量技术(IPTA),处理了福州市2008~2014年间多时相、高分辨率Terra SAR-X数据,对福州市6年时间的地面沉降进行监测分析,根据研究区地面沉降历史、建设发展现状及沉降异常区分布,着重分析了复杂因素影响下福州市地面沉降的时空变化规律。结果表明:福州市总体年均沉降率-15 mm·a-1左右,存在多个明显的快速沉降区;与1960~1990年的监测资料对比发现,沉降中心由地热温泉区向工程密集建设区转移;较大沉降区以快速线性沉降为主;地面沉降特征的变化受到多种复杂因素叠加影响,导致地面沉降空间扩张、速率加剧。该研究成果可为福州市或其他沿海城市地面沉降风险评估、地面沉降防控等提供一定的科学依据和参考。 展开更多
关键词 复杂因素影响 福州市 地面沉降 时空演化 永久散射体雷达干涉测量
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光系统I捕光天线及反应中心、细胞色素b6f复合物的结构与功能研究2011年度报告
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作者 匡廷云 《科技资讯》 2016年第20期176-176,共1页
该研究旨在揭示光合膜色素蛋白复合体——光系统I捕光天线及核心复合体、细胞色素b6f复合物的结构与功能。通过多种光谱学技术研究光能的吸收传递和转化的量子机理,并通过获得膜蛋白晶体,在近原子或原子水平分辨率解析膜蛋白的空间结构... 该研究旨在揭示光合膜色素蛋白复合体——光系统I捕光天线及核心复合体、细胞色素b6f复合物的结构与功能。通过多种光谱学技术研究光能的吸收传递和转化的量子机理,并通过获得膜蛋白晶体,在近原子或原子水平分辨率解析膜蛋白的空间结构,揭示光合作用的物质基础,更深刻地理解光合作用机理。该报告还将研究参与光合膜组装、光能转换和利用及功能调控有关的重要基因以及相应的蛋白的结构与功能,揭示光合作用中光抑制和光氧化调控的分子机理,为提高光能利用效率提供理论依据。 展开更多
关键词 光系统I 捕光天线I 光系统I核心复合体 细胞色素b6f复合物 光合膜蛋白
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The photodamage process of pigments and proteins of PSI complexes from Spinacia Oleracea L.
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作者 WEI Jie YU Hui +4 位作者 LI Liangbi KUANG Tingyun WANG Jushuo GONG Yandao ZHAO Nanming 《Chinese Science Bulletin》 SCIE EI CAS 2001年第21期1812-1816,共5页
Purified PSI complexes from Spinacia Oleracea L. were exposed to the strong light (PFD=2300μmol m-2s-1) for various period. Along with the illumination the photo-damage process of pigments and proteins of PSI complex... Purified PSI complexes from Spinacia Oleracea L. were exposed to the strong light (PFD=2300μmol m-2s-1) for various period. Along with the illumination the photo-damage process of pigments and proteins of PSI complexes was investigated using absorption, fluorescence, circular dichroism (CD) spectroscopy and SDS-PAGE. It was found from the optical absorption spectra that the maximal ab-sorbance of PSI complexes decreased and maximal peaks blue-shifted during the illumination, and the forth derivative spectra demonstrated that the absorbance decreasing at red region mainly resulted from the aborbance decreasing of the long wavelength Chla, implying that the long-wavelength Chla was readily to be bleached. The CD signals contributed by LHCI decreased more rapidly than other CD signals con-tributed by Chla and Carotenoid, indicating that the LHCI was more sensitive to light than core complexes. It was observed by SDS-PAGE that some small polypeptides of PSI complexes were damaged earlier than reaction 展开更多
关键词 psi pigment-protein complex PHOTODAMAGE LHCI absorption SPECTRA CD spectra.
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光系统I捕光天线及反应中心、细胞色素b6f复合物的结构与功能研究立项报告
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作者 秦晓春 《科技资讯》 2016年第20期174-174,共1页
该课题旨在揭示光合膜色素蛋白复合体棗光系统I捕光天线及核心复合体、细胞色素b6f复合物的结构与功能。通过多种光谱学技术研究光能的吸收传递和转化的量子机理,并通过获得膜蛋白晶体,在近原子或原子水平分辨率解析膜蛋白的空间结构,... 该课题旨在揭示光合膜色素蛋白复合体棗光系统I捕光天线及核心复合体、细胞色素b6f复合物的结构与功能。通过多种光谱学技术研究光能的吸收传递和转化的量子机理,并通过获得膜蛋白晶体,在近原子或原子水平分辨率解析膜蛋白的空间结构,揭示光合作用的物质基础,更深刻地理解光合作用机理。该课题还将研究参与光合膜组装、光能转换和利用与功能调控有关的重要基因以及相应的蛋白的结构与功能,揭示光合作用中光抑制和光氧化调控的分子机理,为提高光能利用效率提供理论依据。 展开更多
关键词 光系统I 捕光天线I 光系统I核心复合体 细胞色素b6f复合物 光合膜蛋白
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THE PIGMENT-PROTEIN COMPLEXES OF HIGHER PLANTS MIGRATION IN THYLAKOID AND THE REGULATION OF LIGHT ENERGY DISTRIBUTION BETWEEN PHOTOSYSTEMS
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作者 邓兴旺 梅镇安 《Science China Chemistry》 SCIE EI CAS 1987年第11期1170-1180,共11页
Appressed and non-appressed lamella membranes of Castor bean leaf chloroplasts were separated by non-ionic detergent Triton-X 100.Appressed membranes showed a high oxygen-evolving activity and low chl a/b ratio. Exami... Appressed and non-appressed lamella membranes of Castor bean leaf chloroplasts were separated by non-ionic detergent Triton-X 100.Appressed membranes showed a high oxygen-evolving activity and low chl a/b ratio. Examining with SDS-PTGE and liquid nitrogen temperature fluorescence measurement showed that they contained only PSII and light-harvesting pigment-protein complexes (LHCP),and there was no detectable amount of PSI. Freeze-fracture electromicroscopic observation confirmed that this part was really an appressed lamella membrane. Through divalent cation Mg^(++), the thylakoid membranes were induced to unstack and restack.With the addition of Mg^(++), the fluorescence intensity was changed instantly. We realized that there existed two processes:One was a rapid process which was accomplished within 30 s. The other was a slow process of which the time duration was about 60 min. This dual effects of Mg^(++) had not been reported before.We had analyzed the change of F685/F730 and discussed the possible rneehanis ms of light energy distribution between photosystems. 展开更多
关键词 THE pigment-protein complexES OF HIGHER PLANTS MIGRATION IN THYLAKOID AND THE REGULATION OF LIGHT ENERGY DISTRIBUTION BETWEEN PHOTOSYSTEMS
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THE FUNCTIONAL ROLE OF 23 kD AND 24 kD POLYPEPTIDES OF THYLAKOID IN Mg^(2+)-INDUCED CHANGE OF EXCITATION ENERGY DISTRIBUTION BETWEEN TWO PHOTOSYSTEMS IN THE CHLOROPLASTS FROM Codium fragile 被引量:1
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作者 李良壁 高振泮 +3 位作者 马红 赵鸿儒 翟小京 马桂芝 《Science China Chemistry》 SCIE EI CAS 1992年第12期1453-1462,共10页
In contrast to the chloroplasts from higher plants, Mg^(2+)-induced PS--Ⅱ fluorescence inten-sity increase does not relate to Mg^(2+)-induced surface charge density decrease of thylakoidin the chloroplasts from Codiu... In contrast to the chloroplasts from higher plants, Mg^(2+)-induced PS--Ⅱ fluorescence inten-sity increase does not relate to Mg^(2+)-induced surface charge density decrease of thylakoidin the chloroplasts from Codium fragile. Tbe extraction of the green alga chloroplasts withCa^(2+) to remove the 30--31kD polypeptide (Q_B protein) on the thylakoid surface does notaffect the above Mg^(2+)-induced phenomena. If the Ca^(2+)-treated chloroplasts are further di-gested by trypsin to remove the 23kD and 24kD polypeptides on the membrane surface,the Mg^(2+)-induced fluorescence effect will completely disappear whereas the property ofMg^(2+)-induced surface charge density changes remains unchanged. These results not onlyshow that the 23kDa and 24kDa polypeptides on the thylakoid surface are the specific act-ing sites of the cation that induce Chla fluorescence change, but also demonstrate that thecation-induced change of excitation energy distribution between two photosystems is not con-trolled by the electrostatic property of thylakoid surface in the chloroplasts of Codiumfragile. 展开更多
关键词 THYLAKOID photosystem LIGHT-HARVESTING pigment-protein complex
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