IFN-γ治疗结核的动物实验研究

目的 探讨 IFN- γ对结核的治疗作用。方法 在建立小鼠结核模型的基础上 ,给予小鼠 IFN- γ腹腔内注射 ,采用 RT- PCR法观察小鼠肺组织 IFN-γ的表达 ,病理切片及肺组织内结核菌培养的对比。结果 给予 IFN-γ注射的小鼠病理组织像上也可见结核肉芽肿样病变 ,但对照组织病理切片上可见在渗出病变的基础上 ,出现灶性坏死。两组病变对比出现明显的差异。肺内细菌数也呈现明显的对比。IFN- γ的 m RNA表达 ,实验组较对照组明显减少。结论 IFN-γ是一个有效的增强宿主抵御结核分支杆菌感染的免疫调节细胞因子。

外科手术患者丙型肝炎病毒感染调查

目的 调查外科患者HCV感染情况。方法 采用酶联免疫吸附的方法和逆转录 -聚合酶链反应 (PT -PCR)方法进行HCV测定。结果 2 5 5 0 0例外科患者中HCV感染率为 3 15 %,其中泌尿外科组HCV感染率最高(13 11%) ,显著高于其它科 (P <0 0 1) ;其余外科组与正常组相比较差异不显著 (P >0 0 1)。抽取 5 0 0例HCV感染的病例 ,应用PT -PCR检测HCVRNA ,阳性率为 77 2 %。结论 透析患者是HCV感染的高危人群 ,加强HCV早期感染的检测十分重要。

Expression of local renin and angiotensinogen mRNA in cirrhotic portal hypertensive patient

AIM: To investigate the expression of local renin and angiotensinogen mRNA in cirrhotic portal hypertensive patients.METHODS: The expression of local renin and angiotensinogen mRNA in the liver, splenic artery and vein of PH patients was detected by RT-PCR analysis.RESULTS: Expression of local renin mRNA in the liver of control group was (0.19±0.11), significantly lower than that in splenic artery(0.45±0.17)or splenic vein(0.39±0.12)respectively, (P＜0.05). Expression of local angiotensinogen mRNA in the liver was (0.64±0.21), significantly higher than that in splenic artery(0.41±0.15) or in splenic vein (0.35±0.18)respectively, (P＜0.05). Expression of local renin mRNA in the liver, splenic artery and vein of PH group was (0.78±0.28),(0.86±0.35) and (0.81±0.22) respectively, significantly higher than that in the control group, (P＜0.05). Expression of local angiotensinogen mRNA in the liver, splenic artery and vein of PH group was (0.96±0.25), (0.83±0.18) and (0.79±0.23)respectively, significantly higher than that in the control group,(P＜0.05). There was no significant difference between the liver, splenic artery and vein in the expression of local renin or local angiotensinogen mRNA in PH group, (P＜0.05).CONCLUSION: In normal subjects the expression of local renin and angiotensinogen mRNA was organ specific, but with increase of the expression of LRAS, the organ-specificity became lost in cirrhotic patients. LRAS may contribute to increased resistance of portal vein with liver and formation of splanchnic vasculopathy.