目的探讨第10号染色体同源丢失性磷酸酶-张力蛋白基因(phosphate and tension homology deleted on chromsome ten,PTEN)启动子甲基化与膀胱尿路上皮癌(bladder urothelial cell carcinoma,BUCC)临床特征的关系。方法选取41例BUCC组织...目的探讨第10号染色体同源丢失性磷酸酶-张力蛋白基因(phosphate and tension homology deleted on chromsome ten,PTEN)启动子甲基化与膀胱尿路上皮癌(bladder urothelial cell carcinoma,BUCC)临床特征的关系。方法选取41例BUCC组织及18例正常膀胱组织标本,采用甲基化特异性PCR(methylation specific PCR,MSP)方法检测PTEN基因启动子甲基化水平,采用Western Blot方法检测PTEN蛋白的表达水平,并分析PTEN基因启动子甲基化水平与BUCC不同临床特征的关系。结果BUCC中PTEN蛋白的表达低于正常膀胱组织(P=0.031);BUCC中PTEN基因启动子甲基化率为53.66%(22/41),高于正常膀胱组织的0.00%(0/18)(P=0.000);PTEN基因启动子甲基化在不同性别、年龄患者之间的差异无统计学意义(P=0.149、P=0.813);在不同的临床分期、病理分级及是否有淋巴结转移的患者之间的差异有统计学意义(P=0.008、P=0.021、P=0.038)。结论 PTEN基因启动子甲基化水平与BUCC病理分级、临床分期和淋巴结转移有关,而与性别、年龄无关。展开更多
Objective:To probe into the relationships between PTEN gene expression,the promoter methylation and gastric cancer and its clinical pathological specific features.Methods:We analyzed the PTEN gene promoter methylation...Objective:To probe into the relationships between PTEN gene expression,the promoter methylation and gastric cancer and its clinical pathological specific features.Methods:We analyzed the PTEN gene promoter methylation and mRNA expression status in gastric cancer tissues and its adjacent normal tissues by methylation specific PCR(MSP) and reverse transcription-polymerase chain reaction(RT-PCR) techniques.Results:PTEN promoters in 48.2%(27/56) gastric cancer tissues and 3.6(2/56) adjacent normal tissues were methylated and the PTEN promoter methylation rate in carcinoma tissues was obviously higher(P < 0.05).Of the 2 cases where the adjacent gastric tissues were methylated,the gastric cancer tissues were both methylated.Of the 29 gastric cancers with lymph node metastasis,19 had their PTEN gene promoters methylated and the PTEN gene promoter methylation in cases with lymph node metastasis was obviously higher than that without lymph node metastasis(P < 0.05).RT-PCR result showed that no expression of PTEN mRNA existed in any of the methylated gastric cancer tissues.Conclusion:The expression loss of PTEN gene mRNA in gastric cancers is related to their promoter methylation and might be one of the reasons for the generation,development and metastasis of gastric cancers.展开更多
文摘目的探讨第10号染色体同源丢失性磷酸酶-张力蛋白基因(phosphate and tension homology deleted on chromsome ten,PTEN)启动子甲基化与膀胱尿路上皮癌(bladder urothelial cell carcinoma,BUCC)临床特征的关系。方法选取41例BUCC组织及18例正常膀胱组织标本,采用甲基化特异性PCR(methylation specific PCR,MSP)方法检测PTEN基因启动子甲基化水平,采用Western Blot方法检测PTEN蛋白的表达水平,并分析PTEN基因启动子甲基化水平与BUCC不同临床特征的关系。结果BUCC中PTEN蛋白的表达低于正常膀胱组织(P=0.031);BUCC中PTEN基因启动子甲基化率为53.66%(22/41),高于正常膀胱组织的0.00%(0/18)(P=0.000);PTEN基因启动子甲基化在不同性别、年龄患者之间的差异无统计学意义(P=0.149、P=0.813);在不同的临床分期、病理分级及是否有淋巴结转移的患者之间的差异有统计学意义(P=0.008、P=0.021、P=0.038)。结论 PTEN基因启动子甲基化水平与BUCC病理分级、临床分期和淋巴结转移有关,而与性别、年龄无关。
基金a grant from the National Natural Science Foundation of China(No.30300154)
文摘Objective:To probe into the relationships between PTEN gene expression,the promoter methylation and gastric cancer and its clinical pathological specific features.Methods:We analyzed the PTEN gene promoter methylation and mRNA expression status in gastric cancer tissues and its adjacent normal tissues by methylation specific PCR(MSP) and reverse transcription-polymerase chain reaction(RT-PCR) techniques.Results:PTEN promoters in 48.2%(27/56) gastric cancer tissues and 3.6(2/56) adjacent normal tissues were methylated and the PTEN promoter methylation rate in carcinoma tissues was obviously higher(P < 0.05).Of the 2 cases where the adjacent gastric tissues were methylated,the gastric cancer tissues were both methylated.Of the 29 gastric cancers with lymph node metastasis,19 had their PTEN gene promoters methylated and the PTEN gene promoter methylation in cases with lymph node metastasis was obviously higher than that without lymph node metastasis(P < 0.05).RT-PCR result showed that no expression of PTEN mRNA existed in any of the methylated gastric cancer tissues.Conclusion:The expression loss of PTEN gene mRNA in gastric cancers is related to their promoter methylation and might be one of the reasons for the generation,development and metastasis of gastric cancers.
