Background:The purpose of the study was to investigate the active ingredients and potential biochemical mechanisms of Juanbi capsule in knee osteoarthritis based on network pharmacology,molecular docking and animal ex...Background:The purpose of the study was to investigate the active ingredients and potential biochemical mechanisms of Juanbi capsule in knee osteoarthritis based on network pharmacology,molecular docking and animal experiments.Methods:Chemical components for each drug in the Juanbi capsule were obtained from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,while the target proteins for knee osteoarthritis were retrieved from the Drugbank,GeneCards,and OMIM databases.The study compared information on knee osteoarthritis and the targets of drugs to identify common elements.The data was imported into the STRING platform to generate a protein-protein interaction network diagram.Subsequently,a“component-target”network diagram was created using the screened drug components and target information with Cytoscape software.Common targets were imported into Metascape for GO function and KEGG pathway enrichment analysis.AutoDockTools was utilized to predict the molecular docking of the primary chemical components and core targets.Ultimately,the key targets were validated through animal experiments.Results:Juanbi capsule ameliorated Knee osteoarthritis mainly by affecting tumor necrosis factor,interleukin1β,MMP9,PTGS2,VEGFA,TP53,and other cytokines through quercetin,kaempferol,andβ-sitosterol.The drug also influenced the AGE-RAGE,interleukin-17,tumor necrosis factor,Relaxin,and NF-κB signaling pathways.The network pharmacology analysis results were further validated in animal experiments.The results indicated that Juanbi capsule could decrease the levels of tumor necrosis factor-αand interleukin-1βin the serum and synovial fluid of knee osteoarthritis rats and also down-regulate the expression levels of MMP9 and PTGS2 proteins in the articular cartilage.Conclusion:Juanbi capsule may improve the knee bone microstructure and reduce the expression of inflammatory factors of knee osteoarthritis via multiple targets and multiple signaling pathways.展开更多
目的研究敲减PTGS2对成纤维细胞全基因组表达谱的影响,在基因水平上探索防治瘢痕疙瘩的新途径。方法运用RNAi干扰正常皮肤成纤维细胞前列腺素内过氧化物合酶2(PTGS2)基因的表达,利用real time RT-PCR验证siRNA沉默效果;应用全基因组芯...目的研究敲减PTGS2对成纤维细胞全基因组表达谱的影响,在基因水平上探索防治瘢痕疙瘩的新途径。方法运用RNAi干扰正常皮肤成纤维细胞前列腺素内过氧化物合酶2(PTGS2)基因的表达,利用real time RT-PCR验证siRNA沉默效果;应用全基因组芯片检测基因表达谱变化。结果成纤维细胞的PTGS2基因经siRNA干扰后,其mRNA表达水平明显下调;全基因组芯片表达谱检测到的差异表达基因,按1.5倍差异共189个(115个上调,74个下调),按2倍差异共14个(9个上调,5个下调);基因表达谱的变化与瘢痕疙瘩基因表达谱的变化相吻合,可能促使正常皮肤成纤维细胞向瘢痕疙瘩方向进展。结论检测到与PTGS2基因相关的、在瘢痕疙瘩形成中可能共同发挥作用的相关基因,证明PTGS2与瘢痕疙瘩的发病机制有着密切的关系,为治疗瘢痕疙瘩提供了一个潜在的候选靶点。展开更多
基金funding from the Basic Research Project of the Education Department of Shaanxi Province(21JC010,21JP035)the Young and Middle-Aged Scientific Research and Innovation Team of the Shaanxi Provincial Administration of Traditional Chinese Medicine(2022SLRHLJ001)the 2023 Central Financial Transfer Payment Local Project“Innovation and Improvement of Five Types of Hospital Preparations,Such as Roumudan Granules”.
文摘Background:The purpose of the study was to investigate the active ingredients and potential biochemical mechanisms of Juanbi capsule in knee osteoarthritis based on network pharmacology,molecular docking and animal experiments.Methods:Chemical components for each drug in the Juanbi capsule were obtained from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,while the target proteins for knee osteoarthritis were retrieved from the Drugbank,GeneCards,and OMIM databases.The study compared information on knee osteoarthritis and the targets of drugs to identify common elements.The data was imported into the STRING platform to generate a protein-protein interaction network diagram.Subsequently,a“component-target”network diagram was created using the screened drug components and target information with Cytoscape software.Common targets were imported into Metascape for GO function and KEGG pathway enrichment analysis.AutoDockTools was utilized to predict the molecular docking of the primary chemical components and core targets.Ultimately,the key targets were validated through animal experiments.Results:Juanbi capsule ameliorated Knee osteoarthritis mainly by affecting tumor necrosis factor,interleukin1β,MMP9,PTGS2,VEGFA,TP53,and other cytokines through quercetin,kaempferol,andβ-sitosterol.The drug also influenced the AGE-RAGE,interleukin-17,tumor necrosis factor,Relaxin,and NF-κB signaling pathways.The network pharmacology analysis results were further validated in animal experiments.The results indicated that Juanbi capsule could decrease the levels of tumor necrosis factor-αand interleukin-1βin the serum and synovial fluid of knee osteoarthritis rats and also down-regulate the expression levels of MMP9 and PTGS2 proteins in the articular cartilage.Conclusion:Juanbi capsule may improve the knee bone microstructure and reduce the expression of inflammatory factors of knee osteoarthritis via multiple targets and multiple signaling pathways.
文摘目的研究敲减PTGS2对成纤维细胞全基因组表达谱的影响,在基因水平上探索防治瘢痕疙瘩的新途径。方法运用RNAi干扰正常皮肤成纤维细胞前列腺素内过氧化物合酶2(PTGS2)基因的表达,利用real time RT-PCR验证siRNA沉默效果;应用全基因组芯片检测基因表达谱变化。结果成纤维细胞的PTGS2基因经siRNA干扰后,其mRNA表达水平明显下调;全基因组芯片表达谱检测到的差异表达基因,按1.5倍差异共189个(115个上调,74个下调),按2倍差异共14个(9个上调,5个下调);基因表达谱的变化与瘢痕疙瘩基因表达谱的变化相吻合,可能促使正常皮肤成纤维细胞向瘢痕疙瘩方向进展。结论检测到与PTGS2基因相关的、在瘢痕疙瘩形成中可能共同发挥作用的相关基因,证明PTGS2与瘢痕疙瘩的发病机制有着密切的关系,为治疗瘢痕疙瘩提供了一个潜在的候选靶点。
