Tyrosine phosphorylation is crucial for controlling normal cell growth,survival,intercellular communication,gene transcription,immune responses,and other processes.protein tyrosine phosphatase(PTP)and protein tyrosine...Tyrosine phosphorylation is crucial for controlling normal cell growth,survival,intercellular communication,gene transcription,immune responses,and other processes.protein tyrosine phosphatase(PTP)and protein tyrosine kinases(PTK)can achieve this goal by regulating multiple signaling pathways.Oedaleus decorus asiaticus is an important pest that infests the Mongolian Plateau grassland.We aimed to evaluate the survival rate,growth rate,overall performance,and ovarian developmental morphology of the 4th instar nymphs of O.decorus asiaticus while inhibiting the activity of protein tyrosine phosphatase-1B(PTP1B)and PTK.In addition,the expression and protein phosphorylation levels of key genes in the MAPK signaling pathway and antioxidant enzyme activity were assessed.The results showed no significant differences in survival rate,growth rate,or overall performance between PTP1B inhibitor treatment and control.However,after PTK inhibitor treatment,these indexes were significantly lower than those in the control.The ovarian size of female larvae after 15 days of treatment with PTK inhibitors showed significantly slower development,while female larvae treated with PTP1B exhibited faster ovarian growth than the control group.In comparison to controls and nymphs treated with PTK inhibitors,the expression and phosphorylation levels of key genes in the MAPK signaling pathway under PTP1B inhibitor treatments were significantly higher in 4th instar nymphs.However,reactiveoxygen(ROS)species levels and the activities of NADPH oxidase and other antioxidant enzymes were considerably reduced,although they were significantly greater in the PTK inhibitor treatment.The results suggest that PTP1B and PTK feedback inhibition in the mitogen-activated-protein kinases(MAPK)signal transfer can regulate the physiological metabolism of the insect as well as its developmental rate.These findings can facilitate future uses of PTP1B and PTK inhibitors in controlling insect development to help control pest populations.展开更多
随着电视播出清晰度不断提升,IP化播出系统已经逐渐取代传统SDI播出系统,IP化播出系统的时钟同步问题随之而来,而精确时钟控制协议(Precision Time Protocol,PTP)在IP网络中作为时钟同步技术已经得到广泛应用,本文介绍了PTP时钟同步的...随着电视播出清晰度不断提升,IP化播出系统已经逐渐取代传统SDI播出系统,IP化播出系统的时钟同步问题随之而来,而精确时钟控制协议(Precision Time Protocol,PTP)在IP网络中作为时钟同步技术已经得到广泛应用,本文介绍了PTP时钟同步的相关理论,进一步分析了PTP协议应用于IP化播出系统的可能性。展开更多
随着计算机性能的不断提高和计算机网络技术的进步,广播电视专业视音频节目的制作、播出逐渐具备了在虚拟化系统中部署的条件,可以在虚拟化平台上构建基于SMPTE ST2110系列标准的播出系统。而精确时间协议(Precision Time Protocol,PTP...随着计算机性能的不断提高和计算机网络技术的进步,广播电视专业视音频节目的制作、播出逐渐具备了在虚拟化系统中部署的条件,可以在虚拟化平台上构建基于SMPTE ST2110系列标准的播出系统。而精确时间协议(Precision Time Protocol,PTP)时间同步在基于ST 2110系列标准的播出系统中非常关键。为此,讨论在虚拟化平台上为播出系统各虚拟机实现PTP高精度同步的工具和方法。展开更多
本文探讨了一种采用通用IT服务器平台实现专业媒体网络下IP流时钟同步精度分析和监测方法,实现对基于SMPTE ST 2110、SMPTE ST 2022-6等多种专业媒体网络IP流的时钟锁定精度状态进行实时监测和分析,同时该平台具备多通道IP流同时分析的...本文探讨了一种采用通用IT服务器平台实现专业媒体网络下IP流时钟同步精度分析和监测方法,实现对基于SMPTE ST 2110、SMPTE ST 2022-6等多种专业媒体网络IP流的时钟锁定精度状态进行实时监测和分析,同时该平台具备多通道IP流同时分析的能力,并提供信息上报接口。展开更多
基金the National Key R&D Program of China(2022YFD1400500)the earmarked fund for China Agriculture Research System(CARS-34)the Central Public-interest Scientific Institution Basal Research Fund of China(S2022XM21)。
文摘Tyrosine phosphorylation is crucial for controlling normal cell growth,survival,intercellular communication,gene transcription,immune responses,and other processes.protein tyrosine phosphatase(PTP)and protein tyrosine kinases(PTK)can achieve this goal by regulating multiple signaling pathways.Oedaleus decorus asiaticus is an important pest that infests the Mongolian Plateau grassland.We aimed to evaluate the survival rate,growth rate,overall performance,and ovarian developmental morphology of the 4th instar nymphs of O.decorus asiaticus while inhibiting the activity of protein tyrosine phosphatase-1B(PTP1B)and PTK.In addition,the expression and protein phosphorylation levels of key genes in the MAPK signaling pathway and antioxidant enzyme activity were assessed.The results showed no significant differences in survival rate,growth rate,or overall performance between PTP1B inhibitor treatment and control.However,after PTK inhibitor treatment,these indexes were significantly lower than those in the control.The ovarian size of female larvae after 15 days of treatment with PTK inhibitors showed significantly slower development,while female larvae treated with PTP1B exhibited faster ovarian growth than the control group.In comparison to controls and nymphs treated with PTK inhibitors,the expression and phosphorylation levels of key genes in the MAPK signaling pathway under PTP1B inhibitor treatments were significantly higher in 4th instar nymphs.However,reactiveoxygen(ROS)species levels and the activities of NADPH oxidase and other antioxidant enzymes were considerably reduced,although they were significantly greater in the PTK inhibitor treatment.The results suggest that PTP1B and PTK feedback inhibition in the mitogen-activated-protein kinases(MAPK)signal transfer can regulate the physiological metabolism of the insect as well as its developmental rate.These findings can facilitate future uses of PTP1B and PTK inhibitors in controlling insect development to help control pest populations.
文摘随着电视播出清晰度不断提升,IP化播出系统已经逐渐取代传统SDI播出系统,IP化播出系统的时钟同步问题随之而来,而精确时钟控制协议(Precision Time Protocol,PTP)在IP网络中作为时钟同步技术已经得到广泛应用,本文介绍了PTP时钟同步的相关理论,进一步分析了PTP协议应用于IP化播出系统的可能性。
文摘随着计算机性能的不断提高和计算机网络技术的进步,广播电视专业视音频节目的制作、播出逐渐具备了在虚拟化系统中部署的条件,可以在虚拟化平台上构建基于SMPTE ST2110系列标准的播出系统。而精确时间协议(Precision Time Protocol,PTP)时间同步在基于ST 2110系列标准的播出系统中非常关键。为此,讨论在虚拟化平台上为播出系统各虚拟机实现PTP高精度同步的工具和方法。
