ZNE191 is a new zine finger gene which has a 1107 bp open reading frame (ORF) and encodes a 368amino acid protein including a putative DNA-binding domain of four Cys2His2, zine finder motifs at its C-terminal region. ...ZNE191 is a new zine finger gene which has a 1107 bp open reading frame (ORF) and encodes a 368amino acid protein including a putative DNA-binding domain of four Cys2His2, zine finder motifs at its C-terminal region. The ZNF191 eDNA is located in the chromosome 18q12.1 region where it is known that a variety of hereditary diseases arc related to. Probably, this protein has potential function of stimulating the gene transcription.In order to examine the function and structure of ZNF191 protein, the OHK of ZNf191 and its zinc finger region ZNF191 (243-368) genes were inserted into PTSA-18 expression vector by PCR amplification, then the constructed genes were expressed in the PTSA-18/B121 (DE3) system. The two proteins were purified by DEAE-52, CM-23 and Heparin-Sepharose 4B columns. The pooled proteins showed a single band as assayed by Coomasie Brillian Blue Staining of an SDS/polyacryamide gel.展开更多
基金Project supported by the National Natural Science Foundation of China (Grant No. 29671008).
文摘ZNE191 is a new zine finger gene which has a 1107 bp open reading frame (ORF) and encodes a 368amino acid protein including a putative DNA-binding domain of four Cys2His2, zine finder motifs at its C-terminal region. The ZNF191 eDNA is located in the chromosome 18q12.1 region where it is known that a variety of hereditary diseases arc related to. Probably, this protein has potential function of stimulating the gene transcription.In order to examine the function and structure of ZNF191 protein, the OHK of ZNf191 and its zinc finger region ZNF191 (243-368) genes were inserted into PTSA-18 expression vector by PCR amplification, then the constructed genes were expressed in the PTSA-18/B121 (DE3) system. The two proteins were purified by DEAE-52, CM-23 and Heparin-Sepharose 4B columns. The pooled proteins showed a single band as assayed by Coomasie Brillian Blue Staining of an SDS/polyacryamide gel.
