Ulcerative colitis(UC)is a common inflammatory disease of the gastrointestinal tract.Traditional Chinese medicine(TCM)has long been used in Asia as a treatment for UC and Puerariae Radix(PR)is a reliable anti-diarrhea...Ulcerative colitis(UC)is a common inflammatory disease of the gastrointestinal tract.Traditional Chinese medicine(TCM)has long been used in Asia as a treatment for UC and Puerariae Radix(PR)is a reliable anti-diarrheal therapy.The aims of this study were to investigate the protective effect of PR using the dextran sulfate sodium salt(DSS)-induced UC model in mice and identify molecular mechanisms of PR action.The chemical constituents of PR via ultra-performance liquid chromatography/tandem mass spectrometry and identified potential PR and UC targets using a network pharmacology(NP)approach were obtained to guide mouse experiments.A total of 180 peaks were identified from PR including 48 flavonoids,46 organic acids,14 amino acids,8 phenols,8 carbohydrates,7 alkaloids,6 coumarins and 43 other constituents.NP results showed that caspase-1 was the most dysregulated of the core genes associated with UC.A PR dose of 0.136 mg/g administered to DSS treated mice reversed weight loss and decreased colon lengths found in UC mice.PR also alleviated intestinal mucosal shedding,inflammatory cell infiltration and mucin loss.PR treatment suppressed upregulation of NOD-like receptor protein 3(NLRP3),cysteinyl aspartate-specific proteases-1(caspase-1),apoptosis-associated speck-like(ASC)and gasdermin D(GSDMD)at both the protein and m RNA expression levels.The addition of a small molecule dual-specificity phosphatase inhibitor NSC 95397 inhibited the positive effects of PR.These results indicated that PR exerts a protective effect on DSS-induced colitis by inhibiting NLRP3 inflammasome activation in mice.展开更多
[Objectives]To explore the effects of the compatibility of Radix Puerariae and Radix Rehmanniae on blood glucose and blood lipids in diabetic mouses.[Methods]Diabetic mouse model was established.The body weight and fa...[Objectives]To explore the effects of the compatibility of Radix Puerariae and Radix Rehmanniae on blood glucose and blood lipids in diabetic mouses.[Methods]Diabetic mouse model was established.The body weight and fasting blood glucose of mice were measured after 7 and 14 d of administration,and the biochemical indicators of blood lipids(TC,HDL-C,and LDL-C)were detected after 14 d of administration.[Results]Compared with the Radix Puerariae group and Radix Rehmanniae group,the compatibility group(1:2)had the best hypoglycemic effect(P<0.05),and TC and LDL-C in the compatibility group(2:1)significantly decreased(P<0.05),while HDL-C in the compatibility group(1:1)significantly increased(P<0.05).[Conclusions]Radix Puerariae,Radix Rehmanniae and their combination can reduce the blood glucose of diabetic mice.The compatibility group(1:2)had a significant hypoglycemic effect(P<0.05),and LDL-C in the compatibility group(2:1)significantly declined,while HDL-C in the compatibility group(1:1)rose significantly.展开更多
基于CiteSpace软件和文献计量学方法,以Web of science核心数据库为采集对象,“Pueraria Lobata”等为主题词。将1995—2023年的1683篇文献作为研究对象,对文献的发文量、发文作者、发文国家(地区)、发文机构、出版物等进行数据挖掘。...基于CiteSpace软件和文献计量学方法,以Web of science核心数据库为采集对象,“Pueraria Lobata”等为主题词。将1995—2023年的1683篇文献作为研究对象,对文献的发文量、发文作者、发文国家(地区)、发文机构、出版物等进行数据挖掘。运用关键词共现图谱、关键词聚类图谱、关键词突现分析、关键词时区图等方法进行数据可视化分析。结果显示:(1)葛根研究发文量总体呈现上升趋势;葛根研究的核心作者主要来自于中国、美国、韩国等地;核心作者群与核心机构群逐渐形成;葛根投稿期刊呈现明显的层次划分格局。(2)中国的发文量占发文总量的50%,位居全球第一。中国科学院、中国香港中文大学等机构为世界的葛根研究做出了巨大贡献,但从篇被引频次上分析,仍与其他机构存在一定差距。(3)葛根的研究逐渐从理论走向实践,时区图结果显示,目前葛根淀粉、肠道微生物群、网络药理学等成为新兴研究热点。葛根素、异黄酮等关键词贯穿葛根研究整个发展阶段,逐渐形成以药理学为基础,食品科学、化学、植物科学等多学科综合发展的新模式。展开更多
基金financially supported by the National Natural Science Foundation of China(32172897)Central Significant Changes in the Project at the Corresponding Level(Valuable Resources Capacity-Building for Sustainable Utilization of Traditional Chinese Medicine Program)(2060302)Chinese Herbal Medicine Industry Innovation Team of Shandong Province Agricultural Technology System(SDAIT-20-06)。
文摘Ulcerative colitis(UC)is a common inflammatory disease of the gastrointestinal tract.Traditional Chinese medicine(TCM)has long been used in Asia as a treatment for UC and Puerariae Radix(PR)is a reliable anti-diarrheal therapy.The aims of this study were to investigate the protective effect of PR using the dextran sulfate sodium salt(DSS)-induced UC model in mice and identify molecular mechanisms of PR action.The chemical constituents of PR via ultra-performance liquid chromatography/tandem mass spectrometry and identified potential PR and UC targets using a network pharmacology(NP)approach were obtained to guide mouse experiments.A total of 180 peaks were identified from PR including 48 flavonoids,46 organic acids,14 amino acids,8 phenols,8 carbohydrates,7 alkaloids,6 coumarins and 43 other constituents.NP results showed that caspase-1 was the most dysregulated of the core genes associated with UC.A PR dose of 0.136 mg/g administered to DSS treated mice reversed weight loss and decreased colon lengths found in UC mice.PR also alleviated intestinal mucosal shedding,inflammatory cell infiltration and mucin loss.PR treatment suppressed upregulation of NOD-like receptor protein 3(NLRP3),cysteinyl aspartate-specific proteases-1(caspase-1),apoptosis-associated speck-like(ASC)and gasdermin D(GSDMD)at both the protein and m RNA expression levels.The addition of a small molecule dual-specificity phosphatase inhibitor NSC 95397 inhibited the positive effects of PR.These results indicated that PR exerts a protective effect on DSS-induced colitis by inhibiting NLRP3 inflammasome activation in mice.
基金Supported by the National Innovation Planning Project for University Students in 2022 in Guangxi(S202210599012).
文摘[Objectives]To explore the effects of the compatibility of Radix Puerariae and Radix Rehmanniae on blood glucose and blood lipids in diabetic mouses.[Methods]Diabetic mouse model was established.The body weight and fasting blood glucose of mice were measured after 7 and 14 d of administration,and the biochemical indicators of blood lipids(TC,HDL-C,and LDL-C)were detected after 14 d of administration.[Results]Compared with the Radix Puerariae group and Radix Rehmanniae group,the compatibility group(1:2)had the best hypoglycemic effect(P<0.05),and TC and LDL-C in the compatibility group(2:1)significantly decreased(P<0.05),while HDL-C in the compatibility group(1:1)significantly increased(P<0.05).[Conclusions]Radix Puerariae,Radix Rehmanniae and their combination can reduce the blood glucose of diabetic mice.The compatibility group(1:2)had a significant hypoglycemic effect(P<0.05),and LDL-C in the compatibility group(2:1)significantly declined,while HDL-C in the compatibility group(1:1)rose significantly.
文摘基于CiteSpace软件和文献计量学方法,以Web of science核心数据库为采集对象,“Pueraria Lobata”等为主题词。将1995—2023年的1683篇文献作为研究对象,对文献的发文量、发文作者、发文国家(地区)、发文机构、出版物等进行数据挖掘。运用关键词共现图谱、关键词聚类图谱、关键词突现分析、关键词时区图等方法进行数据可视化分析。结果显示:(1)葛根研究发文量总体呈现上升趋势;葛根研究的核心作者主要来自于中国、美国、韩国等地;核心作者群与核心机构群逐渐形成;葛根投稿期刊呈现明显的层次划分格局。(2)中国的发文量占发文总量的50%,位居全球第一。中国科学院、中国香港中文大学等机构为世界的葛根研究做出了巨大贡献,但从篇被引频次上分析,仍与其他机构存在一定差距。(3)葛根的研究逐渐从理论走向实践,时区图结果显示,目前葛根淀粉、肠道微生物群、网络药理学等成为新兴研究热点。葛根素、异黄酮等关键词贯穿葛根研究整个发展阶段,逐渐形成以药理学为基础,食品科学、化学、植物科学等多学科综合发展的新模式。