采用PCR和RT-PCR方法扩增尖孢镰刀菌古巴专化型(Fusarium oxysporum f sp.cubense,FOC)1号和4号生理小种的pacC基因(FOC1-pacC,FOC4-pacC),对FOC1-pacC和FOC4-pacC相似序列进行搜索和比对,以及对基因编码蛋白进行结构预测,发现FOC1-pacC...采用PCR和RT-PCR方法扩增尖孢镰刀菌古巴专化型(Fusarium oxysporum f sp.cubense,FOC)1号和4号生理小种的pacC基因(FOC1-pacC,FOC4-pacC),对FOC1-pacC和FOC4-pacC相似序列进行搜索和比对,以及对基因编码蛋白进行结构预测,发现FOC1-pacC和FOC4-pacC开放阅读框分别为1 863、1 905 bp,编码634、620个氨基酸,2个小种之间基因序列和氨基酸序列差异明显,FOC1-pacC比FOC4-pacC缺少1个基因片段,FOC-pacC与其相似序列有显著差异,比对缺少2个基因片段;初入侵转录组表达分析说明,2生理小种pacC基因在小种侵染不同品种香蕉过程中表达有差异,FOC1-pacC基因只在粉蕉中检测到表达,而FOC4-pacC则在粉蕉和香蕉中都检测到表达,但在粉蕉中的表达量明显高于香蕉。展开更多
剖宫产子宫切除为胎盘植入性疾病(placenta accreta spectrum,PAS)患者首选的治疗方式。此外,一些替代疗法也在全球范围内兴起。保守治疗的主要目的是减少术中大出血等并发症,同时保留子宫和生育能力。2022年6月,American Journal of Ob...剖宫产子宫切除为胎盘植入性疾病(placenta accreta spectrum,PAS)患者首选的治疗方式。此外,一些替代疗法也在全球范围内兴起。保守治疗的主要目的是减少术中大出血等并发症,同时保留子宫和生育能力。2022年6月,American Journal of Obstetrics and Gynecology刊出一项研究(PACCRETA研究),评估对比PAS保守治疗或子宫切除的围产期结局[1],现简要报道如下。展开更多
Apple valsa canker caused by the Ascomycete fungus Valsa mali is one of the most serious diseases of apple,resulting in huge economic losses in the apple-growing area of China.Previous study found that the pathogen co...Apple valsa canker caused by the Ascomycete fungus Valsa mali is one of the most serious diseases of apple,resulting in huge economic losses in the apple-growing area of China.Previous study found that the pathogen could acidify the infected tissues to make lower ambient pH(from 6.0 to 3.5)for their successfully colonization.The pH signaling transcription factor VmPacC is required for acidification of its environment and for full virulence in V.mali.It is known that the functional cooperation of proteins secreted by V.mali plays pivotal role in its successful colonization of host plants.In this study,we used tandem mass tag(TMT)labeling coupled with LC-MS/MS-based quantitative proteomics to analyze the VmPacC-mediated pH regulation in V.mali,focusing on differentially expressed proteins(DEPs).We identified 222 DEPs specific to VmPacC deletion,and 921 DEPs specific to different pH conditions(pH 6.0 and 3.4).Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses indicated that these DEPs were mainly involved in pathways associated with carbon metabolism,biosynthesis of antibiotics,citrate cycle(TCA cycle),glycolysis/gluconeogenesis,glutathione metabolism,ribosomes,and pentose phosphate pathways.Additionally,we identified 119 DEPs that were shared among the VmPacC deletion mutant and different pH conditions,which were mainly related to energy metabolism pathways,providing the energy required for the hyphal growth and responses to environmental stresses.A protein-protein interaction(PPI)network analysis indicated that most of the shared proteins were mapped to an interaction network with a medium confidence score of 0.4.Notably,one uncharacterized protein(KUI69106.1),and two known proteins(heat shock protein 60(KUI73579.1),aspartate aminotransferase(KUI73864.1))located in the core of the network were highly connected(with≥38 directed edges)with the other shared DEPs.Our results suggest that VmPacC participates in the pathogen’s regulation to ambient pH through the regulation of energy metabolism pathways such as the glycolysis/gluconeogenesis pathway and TCA cycle.Finally,we proposed a sophisticated molecular regulatory network to explain pH decrease in V.mali.Our study,by providing insights into V.mali regulating pH,helps to elucidate the mechanisms of host acidification during pathogen infection.展开更多
文摘剖宫产子宫切除为胎盘植入性疾病(placenta accreta spectrum,PAS)患者首选的治疗方式。此外,一些替代疗法也在全球范围内兴起。保守治疗的主要目的是减少术中大出血等并发症,同时保留子宫和生育能力。2022年6月,American Journal of Obstetrics and Gynecology刊出一项研究(PACCRETA研究),评估对比PAS保守治疗或子宫切除的围产期结局[1],现简要报道如下。
基金supported by grants from the National Natural Science Foundation of China(Grant No.31772115 and U1903206).
文摘Apple valsa canker caused by the Ascomycete fungus Valsa mali is one of the most serious diseases of apple,resulting in huge economic losses in the apple-growing area of China.Previous study found that the pathogen could acidify the infected tissues to make lower ambient pH(from 6.0 to 3.5)for their successfully colonization.The pH signaling transcription factor VmPacC is required for acidification of its environment and for full virulence in V.mali.It is known that the functional cooperation of proteins secreted by V.mali plays pivotal role in its successful colonization of host plants.In this study,we used tandem mass tag(TMT)labeling coupled with LC-MS/MS-based quantitative proteomics to analyze the VmPacC-mediated pH regulation in V.mali,focusing on differentially expressed proteins(DEPs).We identified 222 DEPs specific to VmPacC deletion,and 921 DEPs specific to different pH conditions(pH 6.0 and 3.4).Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses indicated that these DEPs were mainly involved in pathways associated with carbon metabolism,biosynthesis of antibiotics,citrate cycle(TCA cycle),glycolysis/gluconeogenesis,glutathione metabolism,ribosomes,and pentose phosphate pathways.Additionally,we identified 119 DEPs that were shared among the VmPacC deletion mutant and different pH conditions,which were mainly related to energy metabolism pathways,providing the energy required for the hyphal growth and responses to environmental stresses.A protein-protein interaction(PPI)network analysis indicated that most of the shared proteins were mapped to an interaction network with a medium confidence score of 0.4.Notably,one uncharacterized protein(KUI69106.1),and two known proteins(heat shock protein 60(KUI73579.1),aspartate aminotransferase(KUI73864.1))located in the core of the network were highly connected(with≥38 directed edges)with the other shared DEPs.Our results suggest that VmPacC participates in the pathogen’s regulation to ambient pH through the regulation of energy metabolism pathways such as the glycolysis/gluconeogenesis pathway and TCA cycle.Finally,we proposed a sophisticated molecular regulatory network to explain pH decrease in V.mali.Our study,by providing insights into V.mali regulating pH,helps to elucidate the mechanisms of host acidification during pathogen infection.
