目的:比较莫司类药物洗脱支架(LES)与紫杉醇洗脱支架(PES)在冠状动脉小血管病变患者中的疗效。方法:通过PubMed、Web of Science、ClinicalTrials、中国生物医学文献数据库、中国知网、万方数据和维普数据库对与本次研究主题相关的文献...目的:比较莫司类药物洗脱支架(LES)与紫杉醇洗脱支架(PES)在冠状动脉小血管病变患者中的疗效。方法:通过PubMed、Web of Science、ClinicalTrials、中国生物医学文献数据库、中国知网、万方数据和维普数据库对与本次研究主题相关的文献进行检索,对纳入的文献进行方法学质量评价。采用RevMan 5.2及Stata 14.0软件进行相关的统计学分析。结果:八项研究共4738例患者纳入此研究。与PES比较,植入LES能减少主要心血管不良事件(RR=0.64,95%CI:0.53~0.77,Z=4.59,P<0.01)、心肌梗死(RR=0.61,95%CI:0.45~0.82,Z=3.24,P<0.01)、支架内血栓(RR=0.22,95%CI:0.13~0.37,Z=5.71,P<0.01)以及靶病变血运重建(RR=0.56,95%CI:0.44~0.71,Z=4.72,P<0.01)的发生率。与PES比较,LES不能减少心因性死亡(RR=1.08,95%CI:0.62~1.88,Z=0.26,P>0.05)和靶血管血运重建事件的发生率(RR=0.80,95%CI:0.45~1.44,Z=0.74,P>0.05)。结论:对于冠状动脉小血管病变行经皮冠脉介入支架植入术的患者,LES较PES有更好的长期疗效,可作为该类患者的首选支架。展开更多
AIM: To investigate the specific mechanisms of intrinsic and acquired resistance to taxotere (TXT) in pancreatic adenocarcinoma (PAC). METHODS: MTT assay was used to detect the sensitivity of PAC cell line SUIT-2 and ...AIM: To investigate the specific mechanisms of intrinsic and acquired resistance to taxotere (TXT) in pancreatic adenocarcinoma (PAC). METHODS: MTT assay was used to detect the sensitivity of PAC cell line SUIT-2 and its sublines (S-007, S-013, S-020, S-028 and TXT selected SUIT-2 cell line, S2/TXT) to TXT. Mdr1 (P-gp), multidrug resistance associated protein (MRP), lung resistance protein (LRP) and beta-tubulin isotype gene expressions were detected by RT-PCR. The functionality of P-gp and MRP was tested using their specific blocker verapamil (Ver) and indomethacin (IMC), respectively. The transporter activity of P-gp was also confirmed by Rhodamine 123 accumulation assay. RESULTS: S-020 and S2/TXT were found to be significantly resistant to TXT(19 and 9.5-fold to their parental cell line SUIT-2, respectively). RT-PCR demonstrated strong expression of Mdr1 in these two cell lines, but weaker expression or no expression in other cells lines. MRP and LRP expressions were found in most of these cell lines. The TXT-resistance in S2-020 and S2/TXT could be reversed almost completely by Ver, but not by IMC. Flow cytometry showed that Ver increased the accumulation of Rhodamine-123 in these two cell lines. Compared with S-020 and SUIT-2, the levels of beta-tubulin isotype II, III expressions in S-2/TXT were increased remarkably. CONCLUSION: The both intrinsic and acquired TXT-related drug resistance in these PAC cell lines is mainly mediated by P-gp, but had no relationship to MRP and LRP expressions. The increases of beta-tubulin isotype II, III might be collateral changes that occur when the SUIT-2 cells are treated with TXT.展开更多
文摘目的:比较莫司类药物洗脱支架(LES)与紫杉醇洗脱支架(PES)在冠状动脉小血管病变患者中的疗效。方法:通过PubMed、Web of Science、ClinicalTrials、中国生物医学文献数据库、中国知网、万方数据和维普数据库对与本次研究主题相关的文献进行检索,对纳入的文献进行方法学质量评价。采用RevMan 5.2及Stata 14.0软件进行相关的统计学分析。结果:八项研究共4738例患者纳入此研究。与PES比较,植入LES能减少主要心血管不良事件(RR=0.64,95%CI:0.53~0.77,Z=4.59,P<0.01)、心肌梗死(RR=0.61,95%CI:0.45~0.82,Z=3.24,P<0.01)、支架内血栓(RR=0.22,95%CI:0.13~0.37,Z=5.71,P<0.01)以及靶病变血运重建(RR=0.56,95%CI:0.44~0.71,Z=4.72,P<0.01)的发生率。与PES比较,LES不能减少心因性死亡(RR=1.08,95%CI:0.62~1.88,Z=0.26,P>0.05)和靶血管血运重建事件的发生率(RR=0.80,95%CI:0.45~1.44,Z=0.74,P>0.05)。结论:对于冠状动脉小血管病变行经皮冠脉介入支架植入术的患者,LES较PES有更好的长期疗效,可作为该类患者的首选支架。
基金Supported in part by Phne-Poulenc Rorer Pharmaceuticals INC.
文摘AIM: To investigate the specific mechanisms of intrinsic and acquired resistance to taxotere (TXT) in pancreatic adenocarcinoma (PAC). METHODS: MTT assay was used to detect the sensitivity of PAC cell line SUIT-2 and its sublines (S-007, S-013, S-020, S-028 and TXT selected SUIT-2 cell line, S2/TXT) to TXT. Mdr1 (P-gp), multidrug resistance associated protein (MRP), lung resistance protein (LRP) and beta-tubulin isotype gene expressions were detected by RT-PCR. The functionality of P-gp and MRP was tested using their specific blocker verapamil (Ver) and indomethacin (IMC), respectively. The transporter activity of P-gp was also confirmed by Rhodamine 123 accumulation assay. RESULTS: S-020 and S2/TXT were found to be significantly resistant to TXT(19 and 9.5-fold to their parental cell line SUIT-2, respectively). RT-PCR demonstrated strong expression of Mdr1 in these two cell lines, but weaker expression or no expression in other cells lines. MRP and LRP expressions were found in most of these cell lines. The TXT-resistance in S2-020 and S2/TXT could be reversed almost completely by Ver, but not by IMC. Flow cytometry showed that Ver increased the accumulation of Rhodamine-123 in these two cell lines. Compared with S-020 and SUIT-2, the levels of beta-tubulin isotype II, III expressions in S-2/TXT were increased remarkably. CONCLUSION: The both intrinsic and acquired TXT-related drug resistance in these PAC cell lines is mainly mediated by P-gp, but had no relationship to MRP and LRP expressions. The increases of beta-tubulin isotype II, III might be collateral changes that occur when the SUIT-2 cells are treated with TXT.
