为了解中国南海海洋自生固氮菌的种类,作者对采集的南海海底淤泥样品进行了固氮微生物的分离、筛选及鉴定。经过土样沸水加热处理,无氮培养基平板初筛后,对分离获得的细菌固氮酶结构基因nif H进行扩增,并对其固氮酶活性进行检测,最终获...为了解中国南海海洋自生固氮菌的种类,作者对采集的南海海底淤泥样品进行了固氮微生物的分离、筛选及鉴定。经过土样沸水加热处理,无氮培养基平板初筛后,对分离获得的细菌固氮酶结构基因nif H进行扩增,并对其固氮酶活性进行检测,最终获得一株能够产芽孢的固氮细菌。对该菌株进行生理生化性状测定、16S r DNA序列分析(Gen Bank登录号KJ627376),并基于nif H、16S r DNA系统进化树分析,确定该菌为一株固氮类芽孢菌(Paenibacillus sp.)NH-1。本研究表明固氮类芽孢杆菌在海洋中确有分布,海洋自生固氮菌的多样性远远超出人们之前的认识。展开更多
Objective:To evaluate the biodegrading property against carcinogenic azo dyes using bacterial isolates of mangrove sediment.Methods:The bacterial isolates were subjected to submerged fermentation and their growth kine...Objective:To evaluate the biodegrading property against carcinogenic azo dyes using bacterial isolates of mangrove sediment.Methods:The bacterial isolates were subjected to submerged fermentation and their growth kinetics were studied.The potential strain was characterized using 16S rDNA sequencing.Results:In the present study,dye degrading bacterial colonies were isolated from the mangrove sediment samples of Parangipettai estuarine area,Tamil Nadu.Of the 30 morphologically different strains isolated,5 showed antagonistic property.The growth kinetics of the two strains,P1 and G1,which showed potent activity were calculated.One particular isolate(P1)showing promising dye degrading potential in the submerged fermentation was further characterized.The strain was identified as Paenibacillus sp.by 16S rDNA sequencing.Conclusions:This study reveals the less explored microflora of mangrove sediments.The novel strain may further be analyzed and used in the treatment of effluent from dye industry so as to reduce the impact of carcinogenic contaminants.展开更多
文摘为了解中国南海海洋自生固氮菌的种类,作者对采集的南海海底淤泥样品进行了固氮微生物的分离、筛选及鉴定。经过土样沸水加热处理,无氮培养基平板初筛后,对分离获得的细菌固氮酶结构基因nif H进行扩增,并对其固氮酶活性进行检测,最终获得一株能够产芽孢的固氮细菌。对该菌株进行生理生化性状测定、16S r DNA序列分析(Gen Bank登录号KJ627376),并基于nif H、16S r DNA系统进化树分析,确定该菌为一株固氮类芽孢菌(Paenibacillus sp.)NH-1。本研究表明固氮类芽孢杆菌在海洋中确有分布,海洋自生固氮菌的多样性远远超出人们之前的认识。
基金Supported by Department of Biotechnology,Government of India(BT/AAQ/Indo-Norway/183196/2007).
文摘Objective:To evaluate the biodegrading property against carcinogenic azo dyes using bacterial isolates of mangrove sediment.Methods:The bacterial isolates were subjected to submerged fermentation and their growth kinetics were studied.The potential strain was characterized using 16S rDNA sequencing.Results:In the present study,dye degrading bacterial colonies were isolated from the mangrove sediment samples of Parangipettai estuarine area,Tamil Nadu.Of the 30 morphologically different strains isolated,5 showed antagonistic property.The growth kinetics of the two strains,P1 and G1,which showed potent activity were calculated.One particular isolate(P1)showing promising dye degrading potential in the submerged fermentation was further characterized.The strain was identified as Paenibacillus sp.by 16S rDNA sequencing.Conclusions:This study reveals the less explored microflora of mangrove sediments.The novel strain may further be analyzed and used in the treatment of effluent from dye industry so as to reduce the impact of carcinogenic contaminants.
