A new monoterpene glycoside named paeonivayin with other seven known compounds were isolated from the roots of Paeonia delavayi Franch. and their structures were determined by means of spectroscopic studies.
A new triterpenoid. 3 beta. 4 beta. 23-trihydroxy-24, 30-dinorolean-12. 20(29)-dien-28-oic acid. together with five known compounds. 2 alpha. 3 beta. 23-trihydroxy-12-oleanen-28-oic acid-beta -D-glucose glucopyranosyl...A new triterpenoid. 3 beta. 4 beta. 23-trihydroxy-24, 30-dinorolean-12. 20(29)-dien-28-oic acid. together with five known compounds. 2 alpha. 3 beta. 23-trihydroxy-12-oleanen-28-oic acid-beta -D-glucose glucopyranosyl ester. palbinone. 2-hydroxy-benzoic acid, vanillic acid. syringic acid. were isolated from the roots of Paeonia delavayi Franch. Their structures were characterized by spectral analysis.展开更多
Paeonia delavayi is a wild tree peony species endemic to high-altitude regions in southwestern China.Recent agricultural land expansion,however,not only causes its dwindling population size,but also poses a severe thr...Paeonia delavayi is a wild tree peony species endemic to high-altitude regions in southwestern China.Recent agricultural land expansion,however,not only causes its dwindling population size,but also poses a severe threat to its long-term persistence.Since our knowledge of the reproductive biology of P.delavayi is very limited,and some management misconceptions have further exacerbated the already worrisome situation,the aim of the present study is to provide some scientific evidence regarding habitat preference and breeding system of P.delavayi,and to help correct some management misconceptions.Specifically,morphological traits of P.delavayi populations growing in two different habitats(i.e.,the open area versus forest understory),including basal stem diameter,plant height and flower numbers per plant,were measured and compared with each other.A range of floral manipulation experiments was further conducted to study the extent of self-compatibility and pollen limitation.The results showed that P.delavayi preferred the open habitat over forest understory as it generally grew bigger with thickerstems,and produced a higher number of flowers in the former habitat.Therefore,the open habitat should receive immediate priority for conservation action instead of being converted to agricultural land;also,P.delavayi is pollen limited,as evidenced by the fact that experimental flowers receiving supplemental non-self pollen generally displayed elevated seed production than flowers subjected to other treatments(e.g.,non-supplemental self pollen,non-supplemental non-self pollen and supplemental self pollen),which is consistent with reported observations that alpine plants are more likely to be pollen-limited than lowland plants.We suggest that human intervention might be necessary to guarantee the long-term persistence of P.delavayi as harsh alpine environment,intense competition for pollinators and different anthropogenic perturbations co-limit its reproductive success.展开更多
Objectives:This study aimed to evaluate the functional activity and phytochemical composition in the flower petals of Paeonia delavayi(P delavay)in different colors.Materials and Methods:P delavayi petal extracts were...Objectives:This study aimed to evaluate the functional activity and phytochemical composition in the flower petals of Paeonia delavayi(P delavay)in different colors.Materials and Methods:P delavayi petal extracts were prepared by maceration in methanol,including purple petal extract(PPE),red petal extract(RPE),and yellow petal extract(YPE),and their antioxidant activity and x-glucosidase and acetylcholinesterase inhibition activities were evaluated.To correlate these measured activities to phytochemicals in the petals,an ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)-based metabolomics method was applied to profile the compositions in the petals of different colors.Finally,the Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathways database was used to identify the related metabolic pathways that are responsible for the production of these polyphenolic phytochermicals in the petals.Results:The results showed that PPE had the highest total phenolic content,total flavonoid content,and the strongest 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)radical scavenging ability,ferric reducing antioxidant power,and acetylcholin-esterase inhibition ability in all three samples,while YPE showed the strongest 2,2-diphenyl-1-picrylhydrazyl(DPPH)scavenging activity and x-glucosidase inhibition ability.A total of 232 metabolites were detected in the metabolomic analysis,198 of which were flavonoids,chalcones,flavonols,and anthocyanins.Correlation analysis indicated that peonidin-3-O-arabinoside and cyanidin-3-O-arabinoside were the major contribu-tors to the antioxidant activity.Principal component analysis showed a clear separation among these three petals.In addition,a total of 38,98,and 96 differential metabolites were identified in PPE,RPE,and YPE,respectively.Pathway enrichment revealed 6 KEGG pathways that displayed significant enrichment differences,of which the anthocyanin biosynthesis,flavone and flavonol biosynthesis were the most enriched signaling pathways,revealing a potential reason for the differences in met abolic and functional levels among different colors of P delavayi petal.Conclusions:P.delavayi petals in different colors have different metabolite contents and functional activities,of which the anthocyanin,flavone,and flavonol metabolites are critical in its functional activities,suggesting the anthocyanin biosynthesis,flavone and flavonol biosynthesis path-ways are the key pathways responsible for both petal color and bioactive phytochemicals in P delavayi flowers.展开更多
文摘A new monoterpene glycoside named paeonivayin with other seven known compounds were isolated from the roots of Paeonia delavayi Franch. and their structures were determined by means of spectroscopic studies.
文摘A new triterpenoid. 3 beta. 4 beta. 23-trihydroxy-24, 30-dinorolean-12. 20(29)-dien-28-oic acid. together with five known compounds. 2 alpha. 3 beta. 23-trihydroxy-12-oleanen-28-oic acid-beta -D-glucose glucopyranosyl ester. palbinone. 2-hydroxy-benzoic acid, vanillic acid. syringic acid. were isolated from the roots of Paeonia delavayi Franch. Their structures were characterized by spectral analysis.
基金supported by China Special Fund for Forest Scientific Research in the Public Welfare (201204110)the National Natural Science Foundation of China (31460158)the Middle Aged Academic and Technical Leader Project of Yunnan Province (2010CI016)
文摘Paeonia delavayi is a wild tree peony species endemic to high-altitude regions in southwestern China.Recent agricultural land expansion,however,not only causes its dwindling population size,but also poses a severe threat to its long-term persistence.Since our knowledge of the reproductive biology of P.delavayi is very limited,and some management misconceptions have further exacerbated the already worrisome situation,the aim of the present study is to provide some scientific evidence regarding habitat preference and breeding system of P.delavayi,and to help correct some management misconceptions.Specifically,morphological traits of P.delavayi populations growing in two different habitats(i.e.,the open area versus forest understory),including basal stem diameter,plant height and flower numbers per plant,were measured and compared with each other.A range of floral manipulation experiments was further conducted to study the extent of self-compatibility and pollen limitation.The results showed that P.delavayi preferred the open habitat over forest understory as it generally grew bigger with thickerstems,and produced a higher number of flowers in the former habitat.Therefore,the open habitat should receive immediate priority for conservation action instead of being converted to agricultural land;also,P.delavayi is pollen limited,as evidenced by the fact that experimental flowers receiving supplemental non-self pollen generally displayed elevated seed production than flowers subjected to other treatments(e.g.,non-supplemental self pollen,non-supplemental non-self pollen and supplemental self pollen),which is consistent with reported observations that alpine plants are more likely to be pollen-limited than lowland plants.We suggest that human intervention might be necessary to guarantee the long-term persistence of P.delavayi as harsh alpine environment,intense competition for pollinators and different anthropogenic perturbations co-limit its reproductive success.
基金the‘High-level Foreign Experts'Special Project of Yunnan Province Thousand Talents Plan and the‘Yunling Industrial Technology Leading Talents’Special Project of Yunnan Province Ten Thousand Talents Plan(No.[2018]212)the Digitalization,Development and Application of Biotic Resource of the Science and Technology Planning Project of Yunnan Province(No.202002AA 10007)the Scientific Research Fund Project of Yunnan Provincial Department of Education(No.2020Y0412),China.
文摘Objectives:This study aimed to evaluate the functional activity and phytochemical composition in the flower petals of Paeonia delavayi(P delavay)in different colors.Materials and Methods:P delavayi petal extracts were prepared by maceration in methanol,including purple petal extract(PPE),red petal extract(RPE),and yellow petal extract(YPE),and their antioxidant activity and x-glucosidase and acetylcholinesterase inhibition activities were evaluated.To correlate these measured activities to phytochemicals in the petals,an ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)-based metabolomics method was applied to profile the compositions in the petals of different colors.Finally,the Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathways database was used to identify the related metabolic pathways that are responsible for the production of these polyphenolic phytochermicals in the petals.Results:The results showed that PPE had the highest total phenolic content,total flavonoid content,and the strongest 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)radical scavenging ability,ferric reducing antioxidant power,and acetylcholin-esterase inhibition ability in all three samples,while YPE showed the strongest 2,2-diphenyl-1-picrylhydrazyl(DPPH)scavenging activity and x-glucosidase inhibition ability.A total of 232 metabolites were detected in the metabolomic analysis,198 of which were flavonoids,chalcones,flavonols,and anthocyanins.Correlation analysis indicated that peonidin-3-O-arabinoside and cyanidin-3-O-arabinoside were the major contribu-tors to the antioxidant activity.Principal component analysis showed a clear separation among these three petals.In addition,a total of 38,98,and 96 differential metabolites were identified in PPE,RPE,and YPE,respectively.Pathway enrichment revealed 6 KEGG pathways that displayed significant enrichment differences,of which the anthocyanin biosynthesis,flavone and flavonol biosynthesis were the most enriched signaling pathways,revealing a potential reason for the differences in met abolic and functional levels among different colors of P delavayi petal.Conclusions:P.delavayi petals in different colors have different metabolite contents and functional activities,of which the anthocyanin,flavone,and flavonol metabolites are critical in its functional activities,suggesting the anthocyanin biosynthesis,flavone and flavonol biosynthesis path-ways are the key pathways responsible for both petal color and bioactive phytochemicals in P delavayi flowers.
