According to the General Office of the State Council of China, tree peony seeds（TPSs, Paeonia suffruticosa Andr.） are considered an emerging source for edible oil. In Chinese folk medicine, TPSs are used for allevia...According to the General Office of the State Council of China, tree peony seeds（TPSs, Paeonia suffruticosa Andr.） are considered an emerging source for edible oil. In Chinese folk medicine, TPSs are used for alleviating waist and leg pain. In the present study, a simple, accurate and rapid fingerprint method based on high performance liquid chromatography with diode array detection（HPLe-DAD） was developed and validated to quantify the profiles of ten representative compounds in TPSs. All standard calibration curves exhibited good linearity（R2〉0.9995） in the I-IPLC-DAD analysis. The recovery of the standards ranged from 96.37% to 102.10%. The results show that stilbe- noids are the major constituents in TPSs, and their total content ranges from 25.71 mg/g to 54.03 mg/g. Suffruticosol A, suffruticosol B, trans-e-viniferm, ampelopsin E and paeoniflorin are the major components in methanol- water（80：20, volume ratio） extracts with average contents of 3.962, 12.264, 5.826, 14.060 and 12.755 rag/g, respec- tively. Additionally, 14 batches of seeds collected from different regions were used to establish a reference fingerprint Their similarity values were higher than 0.944 except for the sample（0.863） from Linxia. These results collectively indicate that the quantitative HPLC fingerprint method can serve as a prerequisite quality control for TPSs, and is a promising resource for developing new herbal or food products.展开更多
为了推动油用牡丹产业的发展,优化油用牡丹种植技术。以油用牡丹“凤丹”作为试验材料,在聊城大学农学院植物种植园和试验室内进行采集、试验分析,利用不同氮素添加水平:不施氮(N0),施氮5 kg N/hm~2(N1)、10 kg N/hm~2(N2)、15 kg N/hm~...为了推动油用牡丹产业的发展,优化油用牡丹种植技术。以油用牡丹“凤丹”作为试验材料,在聊城大学农学院植物种植园和试验室内进行采集、试验分析,利用不同氮素添加水平:不施氮(N0),施氮5 kg N/hm~2(N1)、10 kg N/hm~2(N2)、15 kg N/hm~2(N3)、20 kg N/hm~2(N4)和25 kg N/hm~2(N5)对牡丹幼苗进行处理,得到了根系性状的数据表明:适量增施氮肥,可有效提高幼苗根系同化物积累量,过量增施氮肥又呈下降趋势;随着氮素的增加,根系总表面积、磷素含量、根尖数和分叉数均表现不同程度先升高后降低的趋势;幼苗根系氮素含量随着施氮量的增加前期上升缓慢、中期无明显差异、后期上升迅速,总体呈不断上升的趋势。因此,为保证“凤丹”牡丹幼苗根系吸收能力和正常发育,进而促进“凤丹”牡丹稳产高产,最佳的施氮量为10 kg N/hm~2(N2)。展开更多
文摘According to the General Office of the State Council of China, tree peony seeds（TPSs, Paeonia suffruticosa Andr.） are considered an emerging source for edible oil. In Chinese folk medicine, TPSs are used for alleviating waist and leg pain. In the present study, a simple, accurate and rapid fingerprint method based on high performance liquid chromatography with diode array detection（HPLe-DAD） was developed and validated to quantify the profiles of ten representative compounds in TPSs. All standard calibration curves exhibited good linearity（R2〉0.9995） in the I-IPLC-DAD analysis. The recovery of the standards ranged from 96.37% to 102.10%. The results show that stilbe- noids are the major constituents in TPSs, and their total content ranges from 25.71 mg/g to 54.03 mg/g. Suffruticosol A, suffruticosol B, trans-e-viniferm, ampelopsin E and paeoniflorin are the major components in methanol- water（80：20, volume ratio） extracts with average contents of 3.962, 12.264, 5.826, 14.060 and 12.755 rag/g, respec- tively. Additionally, 14 batches of seeds collected from different regions were used to establish a reference fingerprint Their similarity values were higher than 0.944 except for the sample（0.863） from Linxia. These results collectively indicate that the quantitative HPLC fingerprint method can serve as a prerequisite quality control for TPSs, and is a promising resource for developing new herbal or food products.
文摘为了推动油用牡丹产业的发展,优化油用牡丹种植技术。以油用牡丹“凤丹”作为试验材料,在聊城大学农学院植物种植园和试验室内进行采集、试验分析,利用不同氮素添加水平:不施氮(N0),施氮5 kg N/hm~2(N1)、10 kg N/hm~2(N2)、15 kg N/hm~2(N3)、20 kg N/hm~2(N4)和25 kg N/hm~2(N5)对牡丹幼苗进行处理,得到了根系性状的数据表明:适量增施氮肥,可有效提高幼苗根系同化物积累量,过量增施氮肥又呈下降趋势;随着氮素的增加,根系总表面积、磷素含量、根尖数和分叉数均表现不同程度先升高后降低的趋势;幼苗根系氮素含量随着施氮量的增加前期上升缓慢、中期无明显差异、后期上升迅速,总体呈不断上升的趋势。因此,为保证“凤丹”牡丹幼苗根系吸收能力和正常发育,进而促进“凤丹”牡丹稳产高产,最佳的施氮量为10 kg N/hm~2(N2)。
