Objective To establish a multiplex polymerase chain reaction (M-PCR) assay for simultaneous detection of pathogens causing genital ulcer disease (GUD). Methods Based on the gene-specific region of the following pathog...Objective To establish a multiplex polymerase chain reaction (M-PCR) assay for simultaneous detection of pathogens causing genital ulcer disease (GUD). Methods Based on the gene-specific region of the following pathogens: Chlamydia trachomatis omp1/ompb, herpes simplex virus (HSV) DNA polymerase, Treponema pallidum tpp47, Haemophilus ducreyi 16s rRNA, four sets of primers were designed and an M-PCR assay was developed to detect four pathogens in one test. The assay was evaluated with diagnostic result of golden standard for each pathogen. Results Of the 51 clinical samples, M-PCR showed slightly higher positive rate (47.1%) of HSV than cell culture (23.6%).Meanwhile, the positive rate of T. pallidum detected by M-PCR and dark-field microscopy was 19.6% (10/51) and 15.7% (8/51), respectively. Only one sample was positive for H. ducreyi and no sample was positive for C. trachomatis detected by both M-PCR assay and culture. Conclusion This primary study indicated that M-PCR assay can simultaneously and rapidly detect the four etiologic pathogens causing GUD.展开更多
Objectives: To develop a method of simultaneous PCRdetection of Haemophilus ducreyi, Treponema pallidum, andHerpes Simplex Virus Types 1 and 2 from genital ulcersamong patients attending STD clinics in Guangzhou, Chin...Objectives: To develop a method of simultaneous PCRdetection of Haemophilus ducreyi, Treponema pallidum, andHerpes Simplex Virus Types 1 and 2 from genital ulcersamong patients attending STD clinics in Guangzhou, China;and evaluate the clinical application of multiplex PCR (M-PCR) assay for diagnosing the etiology of genital ulcerdiseases (GUD). Methods: 244 patients with a genital ulcer were evaluated.Clinical etiology of GUD was based on physical appearanceand microbiologic evaluations that included dark fieldmicroscopy examination (D-F) and serology test for syphilis(STS). Swabs of each genital ulcer were tested for HSVantigen by enzyme immunoassay (EIA) and processed in anM-PCR assay for simultaneous detection of T. pallidum, HSVand H. ducreyi. Results: The standard strains of T. pallidum, HSV and H.ducreyi were amplified by M-PCR, producing amplifiedproducts of 260bp,432bp,170bp, respectively. The sensitivityof M-PCR is 102pg DNA. M-PCR assay for T. pallidum, HSVand H. ducreyi showed good agreement when compared withD-F detection for T. pallidum, STS, H. ducreyi culture and EIAfor HSV antigen (Kappa scores are 0.774,0.704,0.793,0.756,respectively). Conclusions: The M-PCR is a convenient, accurate andreliable assay for the detection of T. pallidum, HSV and H.ducreyi from genital ulcers, and can be used as a method of diagnosing the etiology of GUD.展开更多
Over the past few weeks,we have observed increasing concern about the possible impact of coronavirus disease 2019(COVID-19)which is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2 virus)on male fe...Over the past few weeks,we have observed increasing concern about the possible impact of coronavirus disease 2019(COVID-19)which is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2 virus)on male fertility.Precise mechanisms of male reproductive damages are still unclear,but it seems that high temperature resulting from persistent fever and triggering a secondary autoimmune response leading to an autoimmune orchitis are the most likely involved mechanisms.Also,angiotensin conversion enzyme 2(ACE2)plays a highly important role in cellular entry for SARS-CoV-2 and male genital system presents high ACE2 expression.All these preliminary findings suggest that COVID-19 could impact men's reproductive health.Thus,we examined available data including published and unpublished articles to assess the potential risk of COVID-19 in particular on the male reproductive system.展开更多
Background: Neuroendocrine neoplasms are those that develop from a neuroendocrine cell. They most commonly affect the lungs, gastrointestinal tract, and pancreas, being rare conditions in the female genital tract. Whe...Background: Neuroendocrine neoplasms are those that develop from a neuroendocrine cell. They most commonly affect the lungs, gastrointestinal tract, and pancreas, being rare conditions in the female genital tract. When present, these neoplasms often manifest with nonspecific signs and symptoms such as pain, itching, swelling, single-focus lesions, bleeding, and enlargement of inguinal lymph nodes, in addition to the presence of progressively enlarging vulvar nodules. Consequently, the diagnostic investigation involves histopathological examination and confirmation through immunohistochemistry. Objective: To present a comprehensive understanding of this rarely studied pathology. The primary objective is to provide valuable insights that could aid in the future development of universally applicable treatment guidelines. Case Presentation: A 57-year-old female, with no prior comorbidities, menopause at 36, who presented with a left vulvar nodule accompanied by intense pain and swelling, later diagnosed with small cell neuroendocrine carcinoma in the vulva. Conclusion: This case report highlights the importance of enhancing our knowledge regarding small cell neuroendocrine carcinoma in the vulva, given its scarcity in medical literature. The information presented here underscores the need for standardized diagnostic and treatment approaches, paving the way for future consensus on managing this uncommon but challenging neoplasm.展开更多
Objective: To review our experience in diagnosis, pathology, treatment, and prognosis of extramammary Paget's disease of the vulva. Methods: Seven patients with vulvar Paget's disease who were treated at the C...Objective: To review our experience in diagnosis, pathology, treatment, and prognosis of extramammary Paget's disease of the vulva. Methods: Seven patients with vulvar Paget's disease who were treated at the Cancer Hospital cAMS were retrospectively reviewed for the period from 1960 to 2002. The symptoms, location of disease, pathology, mode of treatment and current status of the patients were evaluated. Results: A total of seven women with Paget抯 disease of the vulva were admitted to the Department of Gynecologic of Oncology. The mean age was 67.3 years (range 54~81 years). Vulvar lesion and pruritus were the common symptoms and it took average 3.4 years to confirm the diagnosis. All patients underwent surgery as initial treatment, vulvectomy was performed for 2 patients and radical vulvectomy plus groin node dissection for 5 patients. Two patients had invasive disease and four were associated with underlying adenocarcinoma. Five patients experienced recurrence, on average, 16.2 months after the surgery. Four of them underwent radiotherapy or chemotherapy and the other received no further treatment. The mean follow-up time was 41.1 months. Three patients, with invasive lesion or underlying adenocarcinoma were dead of the disease. Conclusion: There is a delay in the diagnosis of vulvar Paget's disease. The major treatment is surgery. Recurrence is relatively common. Patients with invasive disease or underlying carcinoma have a poor prognosis.展开更多
目的:了解中国女性1990—2019年妇科疾病负担变化趋势及其年龄、时期、队列效应,为妇科疾病的预防和控制策略的制定提供科学依据。方法:利用2019年全球疾病负担研究(Global Burden of Disease Study 2019,GBD 2019)数据,采用Joinpoint...目的:了解中国女性1990—2019年妇科疾病负担变化趋势及其年龄、时期、队列效应,为妇科疾病的预防和控制策略的制定提供科学依据。方法:利用2019年全球疾病负担研究(Global Burden of Disease Study 2019,GBD 2019)数据,采用Joinpoint回归模型分析中国妇科疾病发病率和伤残调整寿命年(disability adjusted life year,DALY)率的变化趋势,基于年龄-时期-队列模型探讨年龄、时期和队列效应对发病率和DALY率的影响,并分析经前期综合征、生殖器脱垂、子宫肌瘤、子宫内膜异位症、多囊卵巢综合征发病率和DALY率的顺位。结果:1990—2019年中国妇科疾病发病率和DALY率均呈下降趋势,平均每年分别下降0.63%(95%CI:-0.73%~-0.54%,P<0.05)和0.34%(95%CI:-0.43%~-0.24%,P<0.05)。年龄-时期-队列模型结果显示,1990—2019年中国妇科疾病发病率和DALY率的纵向年龄曲线均呈先上升后下降的趋势,在30~34岁达高峰。随着时期的推移,妇科疾病的发病风险和DALY风险均呈下降趋势,以2000—2004年为参考时期(RR=1),2015—2019年时期发病风险下降至最低(RR=0.73,95%CI:0.74~0.71)。1905—1909至1985—1989年出生队列的发病风险和DALY风险呈下降趋势,1990—1994至2000—2004年出生队列略呈上升趋势。1990和2019年常见妇科疾病中经前期综合征发病率(7245.53/10万、5695.02/10万)和DALY率(222.21/10万、197.95/10万)居于首位。结论:我国减少妇科疾病负担的工作已取得一定进展,但疾病负担仍然严重,应加强对年轻女性妇科疾病的健康宣传教育,开展有针对性的普查,提高诊断和治疗技术,继续降低我国的妇科疾病负担。展开更多
970228 The therapeutic effect of purified human leu-cocytic interferon-a on hemorrhagic fever with renalsyndrome. BAI Jingyu(白敬羽), et al. Changzhou 3rdPeople’s Hosp, Changzhou, 213001. Chin J Intern Med1997; 36(2)...970228 The therapeutic effect of purified human leu-cocytic interferon-a on hemorrhagic fever with renalsyndrome. BAI Jingyu(白敬羽), et al. Changzhou 3rdPeople’s Hosp, Changzhou, 213001. Chin J Intern Med1997; 36(2): 90-93. Objective:To explore the therapeutic effect of puri-展开更多
Objective The aim of the study was to analyze the clinical features of patients with perianal Paget’s disease(PPD)and investigate prognosis risk factors.Methods The SEER*Stat software was used to identify 116 PPD pat...Objective The aim of the study was to analyze the clinical features of patients with perianal Paget’s disease(PPD)and investigate prognosis risk factors.Methods The SEER*Stat software was used to identify 116 PPD patients from 1975 to 2015 in the SEER research database.The Kaplan-Meier method was used to conduct a univariate analysis for PPD patients.The differences in survival rates were evaluated using the log-rank test.The differences in the clinicopathological features of PPD patients with or without anorectal carcinoma were compared using the chi-square test.Results The median survival time of PPD patients was 44 months.The median age of onset was 73 years old.The 43.10%of the patients were alive at the end of follow up,and only 12.93%of the patients died of PPD.Elderly(age>70 years;χ^2=9.453,P=0.002),poor differentiation(χ^2=46.557,P=0.000)and abdominal perineal resection(APR;χ^2=46.557,P=0.000)were unfavorable risk factors of prognosis.Nearly 50%of PPD had combined with other malignancies,and over 22.41%of those had multiple primary neoplasms(3 or more).PPDs predisposed concurrent malignancy,and 48.21%of PPD patients with other malignancies combined with anorectal carcinoma in the study.Stage(χ^2=10.127,P=0.018),and surgical method(χ^2=12.245,P=0.007)were statistically significant in the PPD patients with or without anorectal carcinoma.The 16.07%of patients had multiple lesions of Paget’s.Conclusion Patients with PPD have a favorable survival,while the disease-specific mortality is low.Diagnosed age,differentiation,and surgical methods were the influence factors of prognosis in PPD patients.PPDs with anorectal carcinoma is of most important in further investigation.展开更多
[目的]了解性病门诊生殖器溃疡性疾病(GUD)的HIV感染状况及其与HIV感染的关系。[方法]取GUD患者溃疡处分泌物进行暗视野显微镜检查、多重聚合酶链反应,同时取上述患者和非溃疡性性传播疾病(STD)患者尿道或宫颈或阴道分泌物进行淋球菌、...[目的]了解性病门诊生殖器溃疡性疾病(GUD)的HIV感染状况及其与HIV感染的关系。[方法]取GUD患者溃疡处分泌物进行暗视野显微镜检查、多重聚合酶链反应,同时取上述患者和非溃疡性性传播疾病(STD)患者尿道或宫颈或阴道分泌物进行淋球菌、沙眼衣原体、支原体、单纯疱疹病毒、人类乳头瘤病毒、念珠菌、滴虫及加特纳菌等检测;检测所有STD患者血清的HIV抗体及梅毒血清学试验。[结果]在8962例病人中,就诊时生殖器有溃疡的STD与生殖器无溃疡的STD病人中HIV感染率分别为1.75%(5/285)和1.53%(133/8677),两者无统计学意义(x^2=0.09,P>0.05; OR=1.15,95% CI=0.47~2.81);梅毒、生殖器疱疹和其他STD病人的HIV感染率分别为2.81%(22/784)、0.74%(6/814)和1.49%(110/7364),梅毒病人的HIV感染率明显高于生殖器疱疹和其他STD病人的HIV感染率,有统计学意义(2.81% vs 0.74%,x^2=9.92,P<0.05,OR=3.89,95% CI=1.67~9.05;2.81% vs 1.49%,x^2=7.66,P<0.001,OR=1.90,95% CI=1.21~3.00)。[结论]本研究提示本组GUD病人HIV感染率较国外低,梅毒与HIV感染有明显相关性;生殖器疱疹病人与HIV感染的关系尚待进一步研究。展开更多
文摘Objective To establish a multiplex polymerase chain reaction (M-PCR) assay for simultaneous detection of pathogens causing genital ulcer disease (GUD). Methods Based on the gene-specific region of the following pathogens: Chlamydia trachomatis omp1/ompb, herpes simplex virus (HSV) DNA polymerase, Treponema pallidum tpp47, Haemophilus ducreyi 16s rRNA, four sets of primers were designed and an M-PCR assay was developed to detect four pathogens in one test. The assay was evaluated with diagnostic result of golden standard for each pathogen. Results Of the 51 clinical samples, M-PCR showed slightly higher positive rate (47.1%) of HSV than cell culture (23.6%).Meanwhile, the positive rate of T. pallidum detected by M-PCR and dark-field microscopy was 19.6% (10/51) and 15.7% (8/51), respectively. Only one sample was positive for H. ducreyi and no sample was positive for C. trachomatis detected by both M-PCR assay and culture. Conclusion This primary study indicated that M-PCR assay can simultaneously and rapidly detect the four etiologic pathogens causing GUD.
基金Financially supported by Foundation for Major Projects of Guangdong Province(No.99049)Medical Research Foundation of Guangdong Province(No.B2001100)
文摘Objectives: To develop a method of simultaneous PCRdetection of Haemophilus ducreyi, Treponema pallidum, andHerpes Simplex Virus Types 1 and 2 from genital ulcersamong patients attending STD clinics in Guangzhou, China;and evaluate the clinical application of multiplex PCR (M-PCR) assay for diagnosing the etiology of genital ulcerdiseases (GUD). Methods: 244 patients with a genital ulcer were evaluated.Clinical etiology of GUD was based on physical appearanceand microbiologic evaluations that included dark fieldmicroscopy examination (D-F) and serology test for syphilis(STS). Swabs of each genital ulcer were tested for HSVantigen by enzyme immunoassay (EIA) and processed in anM-PCR assay for simultaneous detection of T. pallidum, HSVand H. ducreyi. Results: The standard strains of T. pallidum, HSV and H.ducreyi were amplified by M-PCR, producing amplifiedproducts of 260bp,432bp,170bp, respectively. The sensitivityof M-PCR is 102pg DNA. M-PCR assay for T. pallidum, HSVand H. ducreyi showed good agreement when compared withD-F detection for T. pallidum, STS, H. ducreyi culture and EIAfor HSV antigen (Kappa scores are 0.774,0.704,0.793,0.756,respectively). Conclusions: The M-PCR is a convenient, accurate andreliable assay for the detection of T. pallidum, HSV and H.ducreyi from genital ulcers, and can be used as a method of diagnosing the etiology of GUD.
文摘Over the past few weeks,we have observed increasing concern about the possible impact of coronavirus disease 2019(COVID-19)which is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2 virus)on male fertility.Precise mechanisms of male reproductive damages are still unclear,but it seems that high temperature resulting from persistent fever and triggering a secondary autoimmune response leading to an autoimmune orchitis are the most likely involved mechanisms.Also,angiotensin conversion enzyme 2(ACE2)plays a highly important role in cellular entry for SARS-CoV-2 and male genital system presents high ACE2 expression.All these preliminary findings suggest that COVID-19 could impact men's reproductive health.Thus,we examined available data including published and unpublished articles to assess the potential risk of COVID-19 in particular on the male reproductive system.
文摘Background: Neuroendocrine neoplasms are those that develop from a neuroendocrine cell. They most commonly affect the lungs, gastrointestinal tract, and pancreas, being rare conditions in the female genital tract. When present, these neoplasms often manifest with nonspecific signs and symptoms such as pain, itching, swelling, single-focus lesions, bleeding, and enlargement of inguinal lymph nodes, in addition to the presence of progressively enlarging vulvar nodules. Consequently, the diagnostic investigation involves histopathological examination and confirmation through immunohistochemistry. Objective: To present a comprehensive understanding of this rarely studied pathology. The primary objective is to provide valuable insights that could aid in the future development of universally applicable treatment guidelines. Case Presentation: A 57-year-old female, with no prior comorbidities, menopause at 36, who presented with a left vulvar nodule accompanied by intense pain and swelling, later diagnosed with small cell neuroendocrine carcinoma in the vulva. Conclusion: This case report highlights the importance of enhancing our knowledge regarding small cell neuroendocrine carcinoma in the vulva, given its scarcity in medical literature. The information presented here underscores the need for standardized diagnostic and treatment approaches, paving the way for future consensus on managing this uncommon but challenging neoplasm.
文摘Objective: To review our experience in diagnosis, pathology, treatment, and prognosis of extramammary Paget's disease of the vulva. Methods: Seven patients with vulvar Paget's disease who were treated at the Cancer Hospital cAMS were retrospectively reviewed for the period from 1960 to 2002. The symptoms, location of disease, pathology, mode of treatment and current status of the patients were evaluated. Results: A total of seven women with Paget抯 disease of the vulva were admitted to the Department of Gynecologic of Oncology. The mean age was 67.3 years (range 54~81 years). Vulvar lesion and pruritus were the common symptoms and it took average 3.4 years to confirm the diagnosis. All patients underwent surgery as initial treatment, vulvectomy was performed for 2 patients and radical vulvectomy plus groin node dissection for 5 patients. Two patients had invasive disease and four were associated with underlying adenocarcinoma. Five patients experienced recurrence, on average, 16.2 months after the surgery. Four of them underwent radiotherapy or chemotherapy and the other received no further treatment. The mean follow-up time was 41.1 months. Three patients, with invasive lesion or underlying adenocarcinoma were dead of the disease. Conclusion: There is a delay in the diagnosis of vulvar Paget's disease. The major treatment is surgery. Recurrence is relatively common. Patients with invasive disease or underlying carcinoma have a poor prognosis.
文摘目的:了解中国女性1990—2019年妇科疾病负担变化趋势及其年龄、时期、队列效应,为妇科疾病的预防和控制策略的制定提供科学依据。方法:利用2019年全球疾病负担研究(Global Burden of Disease Study 2019,GBD 2019)数据,采用Joinpoint回归模型分析中国妇科疾病发病率和伤残调整寿命年(disability adjusted life year,DALY)率的变化趋势,基于年龄-时期-队列模型探讨年龄、时期和队列效应对发病率和DALY率的影响,并分析经前期综合征、生殖器脱垂、子宫肌瘤、子宫内膜异位症、多囊卵巢综合征发病率和DALY率的顺位。结果:1990—2019年中国妇科疾病发病率和DALY率均呈下降趋势,平均每年分别下降0.63%(95%CI:-0.73%~-0.54%,P<0.05)和0.34%(95%CI:-0.43%~-0.24%,P<0.05)。年龄-时期-队列模型结果显示,1990—2019年中国妇科疾病发病率和DALY率的纵向年龄曲线均呈先上升后下降的趋势,在30~34岁达高峰。随着时期的推移,妇科疾病的发病风险和DALY风险均呈下降趋势,以2000—2004年为参考时期(RR=1),2015—2019年时期发病风险下降至最低(RR=0.73,95%CI:0.74~0.71)。1905—1909至1985—1989年出生队列的发病风险和DALY风险呈下降趋势,1990—1994至2000—2004年出生队列略呈上升趋势。1990和2019年常见妇科疾病中经前期综合征发病率(7245.53/10万、5695.02/10万)和DALY率(222.21/10万、197.95/10万)居于首位。结论:我国减少妇科疾病负担的工作已取得一定进展,但疾病负担仍然严重,应加强对年轻女性妇科疾病的健康宣传教育,开展有针对性的普查,提高诊断和治疗技术,继续降低我国的妇科疾病负担。
文摘970228 The therapeutic effect of purified human leu-cocytic interferon-a on hemorrhagic fever with renalsyndrome. BAI Jingyu(白敬羽), et al. Changzhou 3rdPeople’s Hosp, Changzhou, 213001. Chin J Intern Med1997; 36(2): 90-93. Objective:To explore the therapeutic effect of puri-
基金Supported by grants from the Scientific Research Staring Foundation for the Returned Overseas Scholars and the Scientific Research Foundation of Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology(No.2019A16)。
文摘Objective The aim of the study was to analyze the clinical features of patients with perianal Paget’s disease(PPD)and investigate prognosis risk factors.Methods The SEER*Stat software was used to identify 116 PPD patients from 1975 to 2015 in the SEER research database.The Kaplan-Meier method was used to conduct a univariate analysis for PPD patients.The differences in survival rates were evaluated using the log-rank test.The differences in the clinicopathological features of PPD patients with or without anorectal carcinoma were compared using the chi-square test.Results The median survival time of PPD patients was 44 months.The median age of onset was 73 years old.The 43.10%of the patients were alive at the end of follow up,and only 12.93%of the patients died of PPD.Elderly(age>70 years;χ^2=9.453,P=0.002),poor differentiation(χ^2=46.557,P=0.000)and abdominal perineal resection(APR;χ^2=46.557,P=0.000)were unfavorable risk factors of prognosis.Nearly 50%of PPD had combined with other malignancies,and over 22.41%of those had multiple primary neoplasms(3 or more).PPDs predisposed concurrent malignancy,and 48.21%of PPD patients with other malignancies combined with anorectal carcinoma in the study.Stage(χ^2=10.127,P=0.018),and surgical method(χ^2=12.245,P=0.007)were statistically significant in the PPD patients with or without anorectal carcinoma.The 16.07%of patients had multiple lesions of Paget’s.Conclusion Patients with PPD have a favorable survival,while the disease-specific mortality is low.Diagnosed age,differentiation,and surgical methods were the influence factors of prognosis in PPD patients.PPDs with anorectal carcinoma is of most important in further investigation.
文摘[目的]了解性病门诊生殖器溃疡性疾病(GUD)的HIV感染状况及其与HIV感染的关系。[方法]取GUD患者溃疡处分泌物进行暗视野显微镜检查、多重聚合酶链反应,同时取上述患者和非溃疡性性传播疾病(STD)患者尿道或宫颈或阴道分泌物进行淋球菌、沙眼衣原体、支原体、单纯疱疹病毒、人类乳头瘤病毒、念珠菌、滴虫及加特纳菌等检测;检测所有STD患者血清的HIV抗体及梅毒血清学试验。[结果]在8962例病人中,就诊时生殖器有溃疡的STD与生殖器无溃疡的STD病人中HIV感染率分别为1.75%(5/285)和1.53%(133/8677),两者无统计学意义(x^2=0.09,P>0.05; OR=1.15,95% CI=0.47~2.81);梅毒、生殖器疱疹和其他STD病人的HIV感染率分别为2.81%(22/784)、0.74%(6/814)和1.49%(110/7364),梅毒病人的HIV感染率明显高于生殖器疱疹和其他STD病人的HIV感染率,有统计学意义(2.81% vs 0.74%,x^2=9.92,P<0.05,OR=3.89,95% CI=1.67~9.05;2.81% vs 1.49%,x^2=7.66,P<0.001,OR=1.90,95% CI=1.21~3.00)。[结论]本研究提示本组GUD病人HIV感染率较国外低,梅毒与HIV感染有明显相关性;生殖器疱疹病人与HIV感染的关系尚待进一步研究。