Panax quinquefolium Linn.,belonging to Panax,Araliaceae,contains a variety of active ingredients.In recent years,many studies have shown that P.quinquefolium Linn.has high value and development prospect in inhibiting ...Panax quinquefolium Linn.,belonging to Panax,Araliaceae,contains a variety of active ingredients.In recent years,many studies have shown that P.quinquefolium Linn.has high value and development prospect in inhibiting colon cancer,lung cancer and breast cancer.Recent advances in studies on chemical components,antitumor effects and mechanisms of P.quinquefolium Linn.are reviewed in the paper.展开更多
BACKGROUND:Previous studies have demonstrated that intracellular Ca^(2+)([Ca^(2+)]_i) overload, excitotoxicity,free radical injury,and nitric oxide toxicity are involved in mechanisms of neuronal death in the ischemic...BACKGROUND:Previous studies have demonstrated that intracellular Ca^(2+)([Ca^(2+)]_i) overload, excitotoxicity,free radical injury,and nitric oxide toxicity are involved in mechanisms of neuronal death in the ischemic brain. OBJECTIVE:To investigate the influence of Panax quinquefolium saponins(PQS) on multiple factors-induced Ca^(2+) overload in the rat pheochromocytoma(PC12) cell line. DESIGN,TIME AND SETTING:Intergroup comparison,in vitro study.The experiment was performed at the Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy,Mudanjiang Medical University between November 2007 and April 2008. MATERIALS:In vitro cultured PC12 cells in the logarithmic phase were assigned into blank control,model,and drug treatment groups(10μmol/L nimodipine;40μg/L,100μg/L,and 250μg/L PQS).Nimodipine was purchased from Jiangsu Yangtze River Pharmacy Group Co., China;PQS(purity>95%,HLPC grade) was provided by School of Basic Medical Sciences,Jilin University.Caffeine,Na_2S_2O_4,L-glutamic acid(Glu),Fura-2/AM,and calcium ionophore A23187 were purchased from Sigma,USA. METHODS:PC12 cells in the model and drug treatment groups were separately incubated in glucose-free Hank's buffered saline solution + Na_2S_20_4(2 mmol/L) for 6 hours,Glu(200μmol/L) plus A23187(0.05μmol/L) for 6 hours,KCI(50 mmol/L) for 1 hour,and caffeine(5 mmol/L) for 3 hours to establish models of intracellular Ca^(2+) overload induced by oxygen and glucose deprivation,Glu,A23187,high K^+,or caffeine.In addition,control cells were incubated in high-glucose DMEM culture medium. MAIN OUTCOME MEASURES:[Ca^(2+)]_i changes in PC12 cells exposed to oxygen-glucose deprivation,Glu,A23187,high K^+,or caffeine were detected using spectrofluorometer. RESULTS:PQS blocked the[Ca^(2+)]_i increase induced by oxygen-glucose deprivation,Glu, A23187,high K^+,or caffeine.In particular,high-dose PQS was most effective(P<0.01).PQS significantly inhibited Glu- or caffeine-induced[Ca^(2+)]_i increases in the absence of extracellular Ca^(2+),but nimodipine did not. CONCLUSION:PQS blocked intracellular Ca^(2+) overload induced by oxygen-glucose deprivation, Glu,A23187,high K^+,or caffeine.This mechanism might be involved in the attenuation of neuronal apoptosis following ischemic brain injury.展开更多
基金supported by the grants from Ministry of Education of China(No.104180)Natural Sciences Foundation of Guangdong(No.31891)Chinese Traditional Medicine Administration of Guangdong,China(No.103041).
基金Special Project of the Central Government Guiding Local Science and Technology Development in Sichuan Province"Genomics Innovation Platform of Southwest Characteristic Chinese Medicine Resources"(2020ZYD058)Guangxi Science and Technology Base and Talents Special Project“Guangxi Superior Chinese Patent Medicine and Ethnic Medicine Development Engineering Technology Research Center”(GK AD20238058).
文摘Panax quinquefolium Linn.,belonging to Panax,Araliaceae,contains a variety of active ingredients.In recent years,many studies have shown that P.quinquefolium Linn.has high value and development prospect in inhibiting colon cancer,lung cancer and breast cancer.Recent advances in studies on chemical components,antitumor effects and mechanisms of P.quinquefolium Linn.are reviewed in the paper.
基金Supported by:the Natural Science Foundation of Heilongjiang Province,No ZA2006-07
文摘BACKGROUND:Previous studies have demonstrated that intracellular Ca^(2+)([Ca^(2+)]_i) overload, excitotoxicity,free radical injury,and nitric oxide toxicity are involved in mechanisms of neuronal death in the ischemic brain. OBJECTIVE:To investigate the influence of Panax quinquefolium saponins(PQS) on multiple factors-induced Ca^(2+) overload in the rat pheochromocytoma(PC12) cell line. DESIGN,TIME AND SETTING:Intergroup comparison,in vitro study.The experiment was performed at the Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy,Mudanjiang Medical University between November 2007 and April 2008. MATERIALS:In vitro cultured PC12 cells in the logarithmic phase were assigned into blank control,model,and drug treatment groups(10μmol/L nimodipine;40μg/L,100μg/L,and 250μg/L PQS).Nimodipine was purchased from Jiangsu Yangtze River Pharmacy Group Co., China;PQS(purity>95%,HLPC grade) was provided by School of Basic Medical Sciences,Jilin University.Caffeine,Na_2S_2O_4,L-glutamic acid(Glu),Fura-2/AM,and calcium ionophore A23187 were purchased from Sigma,USA. METHODS:PC12 cells in the model and drug treatment groups were separately incubated in glucose-free Hank's buffered saline solution + Na_2S_20_4(2 mmol/L) for 6 hours,Glu(200μmol/L) plus A23187(0.05μmol/L) for 6 hours,KCI(50 mmol/L) for 1 hour,and caffeine(5 mmol/L) for 3 hours to establish models of intracellular Ca^(2+) overload induced by oxygen and glucose deprivation,Glu,A23187,high K^+,or caffeine.In addition,control cells were incubated in high-glucose DMEM culture medium. MAIN OUTCOME MEASURES:[Ca^(2+)]_i changes in PC12 cells exposed to oxygen-glucose deprivation,Glu,A23187,high K^+,or caffeine were detected using spectrofluorometer. RESULTS:PQS blocked the[Ca^(2+)]_i increase induced by oxygen-glucose deprivation,Glu, A23187,high K^+,or caffeine.In particular,high-dose PQS was most effective(P<0.01).PQS significantly inhibited Glu- or caffeine-induced[Ca^(2+)]_i increases in the absence of extracellular Ca^(2+),but nimodipine did not. CONCLUSION:PQS blocked intracellular Ca^(2+) overload induced by oxygen-glucose deprivation, Glu,A23187,high K^+,or caffeine.This mechanism might be involved in the attenuation of neuronal apoptosis following ischemic brain injury.
