[Objective] The study was to isolate and identify the pathogen of Pandora neoaphidis and to determine its pathogenicity against turnip aphid （Lipaphis erysimi）.[Method] Based on the morphological characteristics,the...[Objective] The study was to isolate and identify the pathogen of Pandora neoaphidis and to determine its pathogenicity against turnip aphid （Lipaphis erysimi）.[Method] Based on the morphological characteristics,the species of pathogenic fungus was identified.Spore shower method was used to carry out bioassay on the pathogen against turnip aphid.[Result] Primary conidia were ovoid,bitunicate and uninucleate,（24.7±1.4）μm×（10.7±0.9）μm,L/D=2.3±0.2.Secondary conidia had the similar shape with the primary ones,（18.6±2.1）μm×（13.3±1.3）μm,L/D=1.4±0.2.Hyphal body was like mycelium with the diameter of （10.6±0.8）μm.Conidiophores had palmate branch with the diameter of （10.0±0.9）μm.Pseudocystidia was not branched,which had rough base with the diameter of （19.2±1.7）μm,and gradually became more angular towards the apex with the diameter of （8.0±0.9）μm at tips.Rhizoid was like monohyphal shape with the diameter of （21.0±3.0）μm at base,the terminal apex had regular discoid holdfast.No resting spores were observed.The lethal dose of the pathogen against turnip aphid was 18.2/mm2.[Conclusion] The entomopathogenic fungus against turnip aphid was identified to be Pandora neoaphidis,and the pathogen was confirmed to have strong pathogenicity against turnip aphid.展开更多
基金Supported by Key Projects in Shaoguang Institute (200402)~~
文摘[Objective] The study was to isolate and identify the pathogen of Pandora neoaphidis and to determine its pathogenicity against turnip aphid （Lipaphis erysimi）.[Method] Based on the morphological characteristics,the species of pathogenic fungus was identified.Spore shower method was used to carry out bioassay on the pathogen against turnip aphid.[Result] Primary conidia were ovoid,bitunicate and uninucleate,（24.7±1.4）μm×（10.7±0.9）μm,L/D=2.3±0.2.Secondary conidia had the similar shape with the primary ones,（18.6±2.1）μm×（13.3±1.3）μm,L/D=1.4±0.2.Hyphal body was like mycelium with the diameter of （10.6±0.8）μm.Conidiophores had palmate branch with the diameter of （10.0±0.9）μm.Pseudocystidia was not branched,which had rough base with the diameter of （19.2±1.7）μm,and gradually became more angular towards the apex with the diameter of （8.0±0.9）μm at tips.Rhizoid was like monohyphal shape with the diameter of （21.0±3.0）μm at base,the terminal apex had regular discoid holdfast.No resting spores were observed.The lethal dose of the pathogen against turnip aphid was 18.2/mm2.[Conclusion] The entomopathogenic fungus against turnip aphid was identified to be Pandora neoaphidis,and the pathogen was confirmed to have strong pathogenicity against turnip aphid.
