目的探讨血小板体积相关指数(PVIs)、Sestrin2、泛连接蛋白1(Pannexin1)与急性脑梗死患者美国国立卫生研究院卒中量表(NIHSS)评分关系及对溶栓预后的预测效能。方法选取2020年1月—2022年10月莆田九十五医院收治急性脑梗死患者82例,均...目的探讨血小板体积相关指数(PVIs)、Sestrin2、泛连接蛋白1(Pannexin1)与急性脑梗死患者美国国立卫生研究院卒中量表(NIHSS)评分关系及对溶栓预后的预测效能。方法选取2020年1月—2022年10月莆田九十五医院收治急性脑梗死患者82例,均给予静脉溶栓治疗,根据溶栓预后分为良好组49例、较差组33例。比较2组治疗前及治疗后5 d、7 d PVIs、Sestrin2、Pannexin1、NIHSS评分,Pearson分析治疗后5 d、7 d PVIs、Sestrin2、Pannexin1与NIHSS评分的关系,多因素Logistic回归分析急性脑梗死患者溶栓预后的相关影响因素,受试者工作特征曲线(ROC)分析治疗后5 d、7 d PVIs、Sestrin2、Pannexin1及不同联合方案对溶栓预后的预测效能。结果治疗后5 d、7 d较差组PVIs、Sestrin2、Pannexin1、NIHSS评分均高于良好组(治疗后5 d:t/P=5.171/<0.001、6.043/<0.001、8.291/<0.001、6.215/<0.001;治疗后7 d:t/P=10.877/<0.001、6.496/<0.001、7.253/<0.001、11.834/<0.001);治疗后5 d、7 d PVIs、Sestrin2、Pannexin1与NIHSS评分呈正相关(治疗后5 d:r/P=0.818/<0.001、0.762/<0.001、0.806/<0.001;治疗后7 d:r/P=0.832/<0.001、0.796/<0.001、0.810/<0.001);Logistic回归分析结果显示,PVIs高、Sestrin2高、Pannexin1高均是影响溶栓预后的相关危险因素[OR(95%CI)=13.672(2.954~63.282)、8.742(1.628~46.944)、9.857(1.021~95.168),P<0.05];治疗后7 d PVIs、Sestrin2、Pannexin1及三者联合预测急性脑梗死患者溶栓预后的AUC大于治疗后5 d对应的预测方案(Z/P=2.115/0.031、2.102/0.036、4.207/0.030、2.056/0.040),且治疗后5 d三项指标联合的AUC大于各自单独预测(Z/P=3.289/0.002、3.387/0.001、3.562/<0.001),治疗后7 d三项指标联合的AUC大于各自单独预测(Z/P=3.115/0.005、2.896/0.018、3.402/0.001)。结论PVIs、Sestrin2、Pannexin1与急性脑梗死患者神经缺损程度及溶栓预后有关,联合检测治疗后7 d三者水平有望为临床早期预测溶栓预后提供一个有效方案。展开更多
AIM: To evaluate plasma and aqueous levels of adiponutrin and pannexin 1 in patients with and without diabetic retinopathy.METHODS: The study included three age and gendermatched groups of 20 cataract patients with no...AIM: To evaluate plasma and aqueous levels of adiponutrin and pannexin 1 in patients with and without diabetic retinopathy.METHODS: The study included three age and gendermatched groups of 20 cataract patients with no diabetes or additional disease(Group C), 20 cataract patients with diabetes and no retinopathy(Group DM+C), and 20 cataract patients with diabetic retinopathy(Group DR+C).All the patients were examined with respect to body mass index(BMI), fasting plasma glucose, hemoglobin A1c(HbA1c), and lipid profile.Phacoemulsification and intraocular lens(Phaco+IOL) implantation were performed to all patients in all the groups, and aqueous samples were taken during the operation.The plasma and aqueous adiponutrin and pannexin 1 levels were analyzed using enzyme-linked immunosorbent assays.RESULTS: A statistically significant difference was determined between the groups with respect to BMI, fasting plasma glucose, and HbA1c levels(P<0.05 for all parameters tested).The plasma adiponutrin levels of Group DR+C were statistically significantly lower than those of Group C and Group DM+C(P<0.001, P=0.004).No statistically significant difference was determined in the aqueous adiponutrin levels in three groups.The plasma pannexin 1 levels of Groups DM+C and DR+C were statistically significantly lower than those of Group C(both P=0.001).The aqueous pannexin 1 levels of Group DR+C were statistically significantly higher than those of Group C and Group DM+C(P=0.001, P<0.001).CONCLUSION: Adiponutrin and pannexin 1, which play an important role in the pathophysiology of diabetes and obesity, and have a regulatory role in hyperglycemia and insulin resistance.The measurement of adiponutrin and pannexin 1 levels may support clinicians in determining the risk of DR development.展开更多
Pannexin 1(Panx 1),as a large-pore membrane channel,is highly permeable to ATP and other signaling molecules.Previous studies have demonstrated the expression of Panx 1 in the nervous system,including astrocytes,micro...Pannexin 1(Panx 1),as a large-pore membrane channel,is highly permeable to ATP and other signaling molecules.Previous studies have demonstrated the expression of Panx 1 in the nervous system,including astrocytes,microglia,and neurons.However,the distribution and function of Panx 1 in the peripheral nervous system are not clear.Blocking the function of Panx 1 pharmacologically(carbenoxolone and probenecid)or with small interfering RNA targeting pannexins can greatly reduce hypotonicity-induced ATP release.Treatment of Schwann cells with a Ras homolog family member(Rho)GTPase inhibitor and small interfering RNA targeting Rho or cytoskeleton disrupting agents,such as nocodazole or cytochalasin D,revealed that hypotonicity-induced ATP release depended on intracellular RhoA and the cytoskeleton.These findings suggest that Panx 1 participates in ATP release in Schwann cells by regulating RhoA and the cytoskeleton arrangement.This study was approved by the Animal Ethics Committee of Nantong University,China(No.S20180806-002)on August 5,2018.展开更多
文摘目的探讨血小板体积相关指数(PVIs)、Sestrin2、泛连接蛋白1(Pannexin1)与急性脑梗死患者美国国立卫生研究院卒中量表(NIHSS)评分关系及对溶栓预后的预测效能。方法选取2020年1月—2022年10月莆田九十五医院收治急性脑梗死患者82例,均给予静脉溶栓治疗,根据溶栓预后分为良好组49例、较差组33例。比较2组治疗前及治疗后5 d、7 d PVIs、Sestrin2、Pannexin1、NIHSS评分,Pearson分析治疗后5 d、7 d PVIs、Sestrin2、Pannexin1与NIHSS评分的关系,多因素Logistic回归分析急性脑梗死患者溶栓预后的相关影响因素,受试者工作特征曲线(ROC)分析治疗后5 d、7 d PVIs、Sestrin2、Pannexin1及不同联合方案对溶栓预后的预测效能。结果治疗后5 d、7 d较差组PVIs、Sestrin2、Pannexin1、NIHSS评分均高于良好组(治疗后5 d:t/P=5.171/<0.001、6.043/<0.001、8.291/<0.001、6.215/<0.001;治疗后7 d:t/P=10.877/<0.001、6.496/<0.001、7.253/<0.001、11.834/<0.001);治疗后5 d、7 d PVIs、Sestrin2、Pannexin1与NIHSS评分呈正相关(治疗后5 d:r/P=0.818/<0.001、0.762/<0.001、0.806/<0.001;治疗后7 d:r/P=0.832/<0.001、0.796/<0.001、0.810/<0.001);Logistic回归分析结果显示,PVIs高、Sestrin2高、Pannexin1高均是影响溶栓预后的相关危险因素[OR(95%CI)=13.672(2.954~63.282)、8.742(1.628~46.944)、9.857(1.021~95.168),P<0.05];治疗后7 d PVIs、Sestrin2、Pannexin1及三者联合预测急性脑梗死患者溶栓预后的AUC大于治疗后5 d对应的预测方案(Z/P=2.115/0.031、2.102/0.036、4.207/0.030、2.056/0.040),且治疗后5 d三项指标联合的AUC大于各自单独预测(Z/P=3.289/0.002、3.387/0.001、3.562/<0.001),治疗后7 d三项指标联合的AUC大于各自单独预测(Z/P=3.115/0.005、2.896/0.018、3.402/0.001)。结论PVIs、Sestrin2、Pannexin1与急性脑梗死患者神经缺损程度及溶栓预后有关,联合检测治疗后7 d三者水平有望为临床早期预测溶栓预后提供一个有效方案。
文摘AIM: To evaluate plasma and aqueous levels of adiponutrin and pannexin 1 in patients with and without diabetic retinopathy.METHODS: The study included three age and gendermatched groups of 20 cataract patients with no diabetes or additional disease(Group C), 20 cataract patients with diabetes and no retinopathy(Group DM+C), and 20 cataract patients with diabetic retinopathy(Group DR+C).All the patients were examined with respect to body mass index(BMI), fasting plasma glucose, hemoglobin A1c(HbA1c), and lipid profile.Phacoemulsification and intraocular lens(Phaco+IOL) implantation were performed to all patients in all the groups, and aqueous samples were taken during the operation.The plasma and aqueous adiponutrin and pannexin 1 levels were analyzed using enzyme-linked immunosorbent assays.RESULTS: A statistically significant difference was determined between the groups with respect to BMI, fasting plasma glucose, and HbA1c levels(P<0.05 for all parameters tested).The plasma adiponutrin levels of Group DR+C were statistically significantly lower than those of Group C and Group DM+C(P<0.001, P=0.004).No statistically significant difference was determined in the aqueous adiponutrin levels in three groups.The plasma pannexin 1 levels of Groups DM+C and DR+C were statistically significantly lower than those of Group C(both P=0.001).The aqueous pannexin 1 levels of Group DR+C were statistically significantly higher than those of Group C and Group DM+C(P=0.001, P<0.001).CONCLUSION: Adiponutrin and pannexin 1, which play an important role in the pathophysiology of diabetes and obesity, and have a regulatory role in hyperglycemia and insulin resistance.The measurement of adiponutrin and pannexin 1 levels may support clinicians in determining the risk of DR development.
基金This study was supported by the National Natural Science Foundation of China,Nos.31900718(to ZYW),31872773(to GC)the National Key Research and Development Program of China,No.2017YFA0104704(to GC)+2 种基金Basic Research Program of the Education Department of Jiangsu Province of China,Nos.19KJB180024(to ZYW),18KJB180020(to WXS)Postdoctoral Science Foundation of China,No.2019M651925(to ZYW),Jiangsu Students’Platform for Innovation and Entrepreneurship Training Program of China,No.201810304031Z(to YJD)Six Talent Peaks Project in Jiangsu Province of China,No.WSN-007(to WXS).
文摘Pannexin 1(Panx 1),as a large-pore membrane channel,is highly permeable to ATP and other signaling molecules.Previous studies have demonstrated the expression of Panx 1 in the nervous system,including astrocytes,microglia,and neurons.However,the distribution and function of Panx 1 in the peripheral nervous system are not clear.Blocking the function of Panx 1 pharmacologically(carbenoxolone and probenecid)or with small interfering RNA targeting pannexins can greatly reduce hypotonicity-induced ATP release.Treatment of Schwann cells with a Ras homolog family member(Rho)GTPase inhibitor and small interfering RNA targeting Rho or cytoskeleton disrupting agents,such as nocodazole or cytochalasin D,revealed that hypotonicity-induced ATP release depended on intracellular RhoA and the cytoskeleton.These findings suggest that Panx 1 participates in ATP release in Schwann cells by regulating RhoA and the cytoskeleton arrangement.This study was approved by the Animal Ethics Committee of Nantong University,China(No.S20180806-002)on August 5,2018.
