[Objective] This study aimed optimize sequence-related amplified polymor- phism （SRAP）-PCR system for Paphiopedilum hirsutissimum. [Method] Using P. hir- sutissimum leaf as the material, a single-factor test was ado...[Objective] This study aimed optimize sequence-related amplified polymor- phism （SRAP）-PCR system for Paphiopedilum hirsutissimum. [Method] Using P. hir- sutissimum leaf as the material, a single-factor test was adopted to optimize the fac- tors in SRAP-PCR system, including the concentrations of dNTPs, Mg2＋, Taq poly- merase, DNA template and primers. [Result] The optimized SRAP-PCR system con- tained 2.5 μl of 10xPCR buffer, 0.15 mmol/L dNTPs, 2.0 mmol/L Mg2＋, 0.3 mmol/L primer each, 0.3 U Taq polymerase and 1 μl of DNA template. [Conclusion] This system can amplify clear and repeatable DNA profiles, which can be applied for further study about P. hirsutissimum.展开更多
茂兰国家级自然保护区有很丰富的兰花资源，小叶兜兰（Paphiopedilumbar bigerum Tang et Wang）生于海拔800～1500m的石灰岩山丘荫蔽多石之地或岩隙中，被列入IUCN物种红色名录，属于IUCN濒危物种等级濒危（EN）。利用Li-6400便携式光...茂兰国家级自然保护区有很丰富的兰花资源，小叶兜兰（Paphiopedilumbar bigerum Tang et Wang）生于海拔800～1500m的石灰岩山丘荫蔽多石之地或岩隙中，被列入IUCN物种红色名录，属于IUCN濒危物种等级濒危（EN）。利用Li-6400便携式光合作用系统获得了小叶兜兰的光合反应日变化规律，分析植株和周围环境中微量的土壤，以及周围石灰岩的矿物质成分，得出结论，钙耐受和干旱耐受是茂兰国家级自然保护区作为小叶兜兰原生地最显著的特点。周围环境的高温，湿润的空气，高钙的土壤环境在全世界范围比较少见，这些特点是很多喜钙兰花在茂兰分布的主要原因。展开更多
Paphiopedilum dianthum and P.micranthum are two endangered orchid species,with high ornamental and conservation values.They are sympatric species,but their leaf anatomical traits and flowering period have significant ...Paphiopedilum dianthum and P.micranthum are two endangered orchid species,with high ornamental and conservation values.They are sympatric species,but their leaf anatomical traits and flowering period have significant differences.However,it is unclear whether the differences in leaf structure of the two species will affect their adaptabilities to temperature.Here,we investigated the leaf photosynthetic,anatomical,and flowering traits of these two species at three sites with different temperatures(Kunming,16.7±0.2°C;Puer,17.7±0.2°C;Menglun,23.3±0.2°C)in southwest China.Compared with those at Puer and Kunming,the values of light-saturated photosynthetic rate(Pmax).stomatai conductance(gs),leaf thickness(LT),and stomatai density(SD)in both species were lower at Menglun.The values of Pmax,gs,仃,adaxial cuticle thickness(CTad)and SD in P.dianthum were higher than those of P.micranthum at the three sites.Compared with P.dianthum,there were no flowering plants of P.micranthum at Menglun.These results indicated that both species were less resistance to high temperature,and P.dianthum had a stronger adaptability to high-temperature than P.micranthum.Our findings can provide valuable information for the conservation and cultivation of Paphiopedilum species.展开更多
[Objectives]This study aimed to screen the suitable medium and symbiotic fungi for tissue culture of Paphiopedilum dianthum and to establish a rapid propagation system of Paphiopedilum,so as to promote the development...[Objectives]This study aimed to screen the suitable medium and symbiotic fungi for tissue culture of Paphiopedilum dianthum and to establish a rapid propagation system of Paphiopedilum,so as to promote the development of planting industry and commercial application of Paphiopedilum.[Methods]Using P.dianthum as an experimental materials,the optimal culture medium for P.dianthum was selected from DE medium,Harvais modified medium and(bark+DE)liquid medium.Then,six fungal species isolated from the original habitat soil of Paphiopedilum were co-cultured with P.dianthum on the selected optimal culture medium.By comparing the morphology,the growth amount and the physiological indexes,such as chlorophyll content,soluble sugar content and root vitality of tissue culture plantlets of P.dianthum co-cultured with the six fungal species,the suitable fungal strains for symbiosis with P.dianthum were screened out.[Results]In three kinds of culture media,P.dianthum grew normally.The growth rate was higher than that in the mediums containing DE,and there were no significant differences in the content of soluble sugar from the leaves of tissue culture plantlets,which were symbiotic with the six different fungi species.Among the six fungi species growing on the optimal medium,the growing rate of XH-A was the fastest,and that of YY001 was the slowest.The growth and physiological indexes of P.dianthum co-cultured with DY1 in DE medium were higher than those in the control group.[Conclusions]DE medium was the optimal tissue culture medium for P.dianthum,and the optimal symbiotic fungus was DY1.展开更多
Paphiopedilum armeniacum is a rare and endangered lady’s slipper orchid in China.It is distributed around the mid-elevations of the Nu Mountains in southwest China.Due to over-harvest,habitat loss,and degradation,wil...Paphiopedilum armeniacum is a rare and endangered lady’s slipper orchid in China.It is distributed around the mid-elevations of the Nu Mountains in southwest China.Due to over-harvest,habitat loss,and degradation,wild populations of P.armeniacum has declined drastically.A combination of approaches involving biotechnology,habitat restoration,and interspecific relationship reconstruction was used to carry out the reintroduction of the species.Integrated conservation program for this species included in-situ protection,ex-situ conservation,and reintroduction,which helped to rebuild a harmonious relationship between local farmers and P.armeniacum.The sustainable utilization of native plant resources in poor areas can promote regional sustainable development which is compatible with species protection.展开更多
基金Supported by Scientific Research Fund for the Introduced Talents in Hechi University(2008QB-N001)~~
文摘[Objective] This study aimed optimize sequence-related amplified polymor- phism （SRAP）-PCR system for Paphiopedilum hirsutissimum. [Method] Using P. hir- sutissimum leaf as the material, a single-factor test was adopted to optimize the fac- tors in SRAP-PCR system, including the concentrations of dNTPs, Mg2＋, Taq poly- merase, DNA template and primers. [Result] The optimized SRAP-PCR system con- tained 2.5 μl of 10xPCR buffer, 0.15 mmol/L dNTPs, 2.0 mmol/L Mg2＋, 0.3 mmol/L primer each, 0.3 U Taq polymerase and 1 μl of DNA template. [Conclusion] This system can amplify clear and repeatable DNA profiles, which can be applied for further study about P. hirsutissimum.
文摘茂兰国家级自然保护区有很丰富的兰花资源，小叶兜兰（Paphiopedilumbar bigerum Tang et Wang）生于海拔800～1500m的石灰岩山丘荫蔽多石之地或岩隙中，被列入IUCN物种红色名录，属于IUCN濒危物种等级濒危（EN）。利用Li-6400便携式光合作用系统获得了小叶兜兰的光合反应日变化规律，分析植株和周围环境中微量的土壤，以及周围石灰岩的矿物质成分，得出结论，钙耐受和干旱耐受是茂兰国家级自然保护区作为小叶兜兰原生地最显著的特点。周围环境的高温，湿润的空气，高钙的土壤环境在全世界范围比较少见，这些特点是很多喜钙兰花在茂兰分布的主要原因。
基金This work was financially supported by the National Natural Science Foundation of China(31970361)the Applied Basic Research Plan of Yunnan Province(2018EA016)+2 种基金the Science and Technology Plan of Yunnan(2018BB010)and the project for Construction of International Flower Technology Innovation Center and Achievement Industrialization(2019ZG006)and the Project for Innovation Team of Yunnan Province.Thanks to De John A Meadows for proofreading and editing.Thanks to Me Jianbo Yang for helping to make the map of species distribution.
文摘Paphiopedilum dianthum and P.micranthum are two endangered orchid species,with high ornamental and conservation values.They are sympatric species,but their leaf anatomical traits and flowering period have significant differences.However,it is unclear whether the differences in leaf structure of the two species will affect their adaptabilities to temperature.Here,we investigated the leaf photosynthetic,anatomical,and flowering traits of these two species at three sites with different temperatures(Kunming,16.7±0.2°C;Puer,17.7±0.2°C;Menglun,23.3±0.2°C)in southwest China.Compared with those at Puer and Kunming,the values of light-saturated photosynthetic rate(Pmax).stomatai conductance(gs),leaf thickness(LT),and stomatai density(SD)in both species were lower at Menglun.The values of Pmax,gs,仃,adaxial cuticle thickness(CTad)and SD in P.dianthum were higher than those of P.micranthum at the three sites.Compared with P.dianthum,there were no flowering plants of P.micranthum at Menglun.These results indicated that both species were less resistance to high temperature,and P.dianthum had a stronger adaptability to high-temperature than P.micranthum.Our findings can provide valuable information for the conservation and cultivation of Paphiopedilum species.
基金Project of Construction of Advanced Horticulture Discipline Under Beijing Municipality(2020)Key Laboratory of Biological and Genetic Improvement of Horticultural Crops under Ministry of Agriculture。
文摘[Objectives]This study aimed to screen the suitable medium and symbiotic fungi for tissue culture of Paphiopedilum dianthum and to establish a rapid propagation system of Paphiopedilum,so as to promote the development of planting industry and commercial application of Paphiopedilum.[Methods]Using P.dianthum as an experimental materials,the optimal culture medium for P.dianthum was selected from DE medium,Harvais modified medium and(bark+DE)liquid medium.Then,six fungal species isolated from the original habitat soil of Paphiopedilum were co-cultured with P.dianthum on the selected optimal culture medium.By comparing the morphology,the growth amount and the physiological indexes,such as chlorophyll content,soluble sugar content and root vitality of tissue culture plantlets of P.dianthum co-cultured with the six fungal species,the suitable fungal strains for symbiosis with P.dianthum were screened out.[Results]In three kinds of culture media,P.dianthum grew normally.The growth rate was higher than that in the mediums containing DE,and there were no significant differences in the content of soluble sugar from the leaves of tissue culture plantlets,which were symbiotic with the six different fungi species.Among the six fungi species growing on the optimal medium,the growing rate of XH-A was the fastest,and that of YY001 was the slowest.The growth and physiological indexes of P.dianthum co-cultured with DY1 in DE medium were higher than those in the control group.[Conclusions]DE medium was the optimal tissue culture medium for P.dianthum,and the optimal symbiotic fungus was DY1.
基金This work was supported by the Special Fund for Ecological Forestry Construction of Guangdong Provincal Finance Department[Yue Cai Zi Huan[2021]15]the Central Finance Fund for Forestry Reform and Development[Yue Cai Zi Huan[2019]5,[2020]99].
文摘Paphiopedilum armeniacum is a rare and endangered lady’s slipper orchid in China.It is distributed around the mid-elevations of the Nu Mountains in southwest China.Due to over-harvest,habitat loss,and degradation,wild populations of P.armeniacum has declined drastically.A combination of approaches involving biotechnology,habitat restoration,and interspecific relationship reconstruction was used to carry out the reintroduction of the species.Integrated conservation program for this species included in-situ protection,ex-situ conservation,and reintroduction,which helped to rebuild a harmonious relationship between local farmers and P.armeniacum.The sustainable utilization of native plant resources in poor areas can promote regional sustainable development which is compatible with species protection.
