目的利用共焦显微镜观察比较准分子激光原位角膜磨镶术(laser in situ keratomileusis,LASIK)与准分子激光双面角膜磨镶术(both-sides LASIK,BSL)后角膜上皮下神经纤维的再生情况。方法观察在我院行LASIK或BSL手术的患者21例(38眼),其中...目的利用共焦显微镜观察比较准分子激光原位角膜磨镶术(laser in situ keratomileusis,LASIK)与准分子激光双面角膜磨镶术(both-sides LASIK,BSL)后角膜上皮下神经纤维的再生情况。方法观察在我院行LASIK或BSL手术的患者21例(38眼),其中,LASIK组11例(22眼),BSL组10例(16眼)。手术患者分别在术后1d、1周、1个月、3个月、6个月时行角膜共焦显微镜检查,比较两组患者术后角膜上皮下神经纤维再生的情况。结果术后1d,角膜神经纤维多表现为长的、残留变性神经纤维与被切断的、短的、不连续的神经纤维同时存在;术后1周,两组患者角膜再生神经纤维的长度表现以≤50μm或无神经纤维为主;自术后1个月起,就可见有长度>200μm的再生神经纤维出现,且随着时间延长,所占比例逐渐增加。在术后1个月、3个月、6个月,LASIK组与BSL组角膜上皮下再生神经纤维达到200μm的比例分别为36.3%和12.5%,36.4%和25%,63.6%和68.8%,两组间差异没有统计学意义(P均>0.05)。结论虽然在等效球镜度、术前及术后角膜厚度、手术切削深度等方面,两组之间差异存在统计学意义,但LASIK组与BSL组术后角膜上皮下神经纤维再生长度和形态学在各时间点差异无统计学意义。展开更多
Previous animal studies of cauda equina injury have primarily used rat models, which display significant differences from humans. Furthermore, most studies have focused on electrophysio- logical examination. To better...Previous animal studies of cauda equina injury have primarily used rat models, which display significant differences from humans. Furthermore, most studies have focused on electrophysio- logical examination. To better mimic the outcome after surgical repair of cauda equina injury, a novel animal model was established in the goat. Electrophysiological, histological and magnetic resonance imaging methods were used to evaluate the morphological and functional outcome after cauda equina injury and end-to-end suture. Our results demonstrate successful establish- ment of the goat experimental model of cauda equina injury. This novel model can provide detailed information on the nerve regenerative process following surgical repair of cauda equina injury.展开更多
文摘目的利用共焦显微镜观察比较准分子激光原位角膜磨镶术(laser in situ keratomileusis,LASIK)与准分子激光双面角膜磨镶术(both-sides LASIK,BSL)后角膜上皮下神经纤维的再生情况。方法观察在我院行LASIK或BSL手术的患者21例(38眼),其中,LASIK组11例(22眼),BSL组10例(16眼)。手术患者分别在术后1d、1周、1个月、3个月、6个月时行角膜共焦显微镜检查,比较两组患者术后角膜上皮下神经纤维再生的情况。结果术后1d,角膜神经纤维多表现为长的、残留变性神经纤维与被切断的、短的、不连续的神经纤维同时存在;术后1周,两组患者角膜再生神经纤维的长度表现以≤50μm或无神经纤维为主;自术后1个月起,就可见有长度>200μm的再生神经纤维出现,且随着时间延长,所占比例逐渐增加。在术后1个月、3个月、6个月,LASIK组与BSL组角膜上皮下再生神经纤维达到200μm的比例分别为36.3%和12.5%,36.4%和25%,63.6%和68.8%,两组间差异没有统计学意义(P均>0.05)。结论虽然在等效球镜度、术前及术后角膜厚度、手术切削深度等方面,两组之间差异存在统计学意义,但LASIK组与BSL组术后角膜上皮下神经纤维再生长度和形态学在各时间点差异无统计学意义。
基金supported by grants from the National Program on Key Basic Research Project of China(973 Program),No.2014CB542200Program for Innovative Research Team in University of Ministry of Education of China,No.IRT1201+2 种基金the National Natural Science Foundation of China,No.31271284,31171150,81171146,30971526,31040043,31371210,81372044,31471144Program for New Century Excellent Talents in University of Ministry of Education of China,No.BMU20110270the Natural Science Foundation of Beijing of China,No.7142164
文摘Previous animal studies of cauda equina injury have primarily used rat models, which display significant differences from humans. Furthermore, most studies have focused on electrophysio- logical examination. To better mimic the outcome after surgical repair of cauda equina injury, a novel animal model was established in the goat. Electrophysiological, histological and magnetic resonance imaging methods were used to evaluate the morphological and functional outcome after cauda equina injury and end-to-end suture. Our results demonstrate successful establish- ment of the goat experimental model of cauda equina injury. This novel model can provide detailed information on the nerve regenerative process following surgical repair of cauda equina injury.
