Analysis of the interactive meaning of journalistic images of the human papillomavirus vaccine and the perceptions of female undergraduate students

Objectives:This paper focuses on the underlying mechanisms of women's perceptions of persuasive visual health information.Methods:In the image viewing process,a separation between the image producer and the image viewer occurs,and the connection between the two is fractured.This mixed method research included modal discourse analysis(coding based on visual grammar theory),an eye tracking experiment,a questionnaire survey,and in-depth semi-structured interviews.The interactive meanings of journalistic images related to the human papillomavirus(HPV)vaccine were identified through four sets of codes.In addition,the perceptions of female viewers were analyzed.Results:In the first set of stimuli,i.e.,the infographic,the female participants focused most of their attention on information about the nine-valent HPV vaccine.An analysis of the interactive meaning of two sets of journalistic pictures,i.e.,fictional pictures and nonfictional pictures,indicated that the image producers did not implement useful viewer involvement strategies to persuade viewers.Furthermore,female viewers focused their attention on the"similar other"during the viewing process,gazing at the patient the longest as the primary area of interest(AOI).Conclusions:The study indicates that the current persuasive visual information about the HPV vaccine needs further improvement due to the high demand for information about HPV from the Chinese female audience.

Monoarthropathy or Polyarthritis in Adolescent Japanese Girls Who Received Immunization with the Human Papillomavirus Vaccine

Joint pain or arthralgia is a common complaint among girls who have received immunization with the human papillomavirus (HPV) vaccine, but the pathogenesis of this disorder has not been completely understood. We report 2 cases of joint lesions after HPV vaccination. In one case, a 13-year-old patient showed transient arthropathy in the right wrist joint after the first dose of Gardasil® administered in her left shoulder. In the other case, an 18-year-old patient had migrating joint pain with redness and swelling after the third dose of Cervarix®. Her serum C-reactive protein and anti-MMP-3 levels were slightly elevated, but no autoantibodies, including rheumatoid factor and anti-CCP antibody, were detected. Although various nonsteroidal anti-inflammatory drugs produced no relief, small doses of both tacrolimus and prednisolone were highly effective for her polyarthritis. The development of reactive joint lesions after HPV vaccination was noteworthy.

Cervical Cancer Prevention Challenges and Barriers to Cervical Cancer Screening and HPV Vaccinations in Ukraine and Eastern Europe

Objectives: To identify the obstacles and issues that hinder effective cancer prevention efforts in Ukraine. The study aims to provide a comprehensive assessment of the barriers to cancer prevention, including both infrastructure and behavioral factors, and identify potential solutions to address these challenges. Study Design: Comprehensive literature review. Methods: The following databases were searched: National Center for Biotechnology Information (NCBI) and PubMed (U.S. National Library of Medicine at the National Institutes of Health). The keywords used in the search included “Cervical Cancer”, “Human Papillomavirus Vaccination (HPV)”, “Ukraine”, “Eastern Europe”, “Healthcare Infrastructure in Disasters”, “Cervical Cancer Prevention”, “Pap Smear”, and “HPV Testing”. Results: A total of 3500 articles were screened. A total of 65 articles met the inclusion criteria. Limited public awareness and limited access to vaccination and screening, combined with inadequate treatment facilities lead to higher rates of cervical cancer. The COVID pandemic, war with Russia, and the Chernobyl disaster are significant factors for the low level of vaccination in Ukraine. Conclusion: The prevention and treatment of cervical cancer in Ukraine face significant challenges due to the inadequate HPV vaccination rates and screening by cytology. Efforts to improve funding and increase education of both the population and health care providers are necessary to increase interventions such as HPV vaccination, cervical cytology, and HPV testing to reduce cervical cancer rates in Ukraine.

Gardasil on Twitter: A Content Mining Study Examining Message, Context, and Source Characteristics of Human Papilloma Virus (HPV) Vaccine-Related Tweets

Genital human papilloma virus (HPV) is the most common sexually transmitted virus in the US. Effective HPV vaccines remain controversial and have poor uptake among many adolescent groups. Social media have potential for promoting the HPV vaccine, but little is known about the content or sentiment of social media discussions regarding the vaccine. The purpose of this study was to examine message, context, sentiment, and source characteristics of original HPV- and HPV vaccine-related Twitter posts (tweets) over a 3-month time period. This content mining study used publicly available data purchased from Twitter. One-thousand tweets were randomly selected from a sample of 45,260 English language tweets matching our search criteria and collected from December 2014-January 2015. Tweets were manually coded according to source, context, message, and sentiment characteristics using a coding document developed from previous research. Individuals posted just over half (55%) of tweets, whereas organizations posted 40%. More than half (57%) of all coded tweets were positive, and less than one-fifth were negative. Organizations’ tweets were more likely to be positive than individuals’ tweets. A temporal display of contextual events and tweet spikes demonstrates that positive tweets can increase during newsworthy events (e.g. FDA approval of Gardisil 9). The HPV vaccine was most often discussed under a cancer prevention message frame. In contrast with some existing research, discussions surrounding the HPV vaccine in original Twitter posts are largely positive. Our study reveals opportunities for adolescent health specialists to provide accurate, positive HPV vaccination information via Twitter.

HPV Vaccination in Young Girls from Developing Countries: What Are the Barriers for Its Implementation? A Systematic Review

Cervical cancer is the second most common cancer in women living in developing countries that account high HPV incidence and mortality rates. Vaccinating girls between 9 and 14 years old is supposed to be the most cost-effective public health approach against cervical cancer. This systematic review aims to assess the application and coverage of the HPV vaccine in developing countries and identify the main challenges for the introduction of the vaccine in these settings. Eligible studies were selected according to the defined inclusion and exclusion criteria. To determine the quality of the studies was employed the STROBE checklist. This review included seven studies, encompassing the analysis of 19 countries and 112,116 girls aged from 9 to 18 years old. The coverage of HPV vaccination ranged from 13.8% to 107.4%, with most of the programs having more than 60% of coverage, which reflects a high percentage of vaccinated girls. The main challenges were lack of knowledge and worries about the vaccine, insufficient financial resources and staff workers, lack of community involvement and dissemination of important information about HPV. In developing countries that implemented the HPV vaccine, high coverage rates were achieved, despite sociocultural, economic and political challenges. In the future, studies that analyze the coverage rates after the elimination of the barriers and the repercussions on the mortality rates should be conducted, so that more developing countries have the opportunity to efficiently implement the vaccine.

Clinician Misperceptions about the Importance of Adolescent HPV Vaccination

Introduction: Adolescent HPV vaccination rates remain suboptimal. The purpose of the study was to investigate attitudes about HPV vaccine relative to other adolescent vaccines among clinical staff from primary care offices and school based clinics. Methods: We interviewed clinicians in primary care offices and school-based clinics regarding their attitudes about HPV vaccine relative to Tdap and MCV4. Results: Respondents (n = 36) included clinical staff in family medicine (47%), pediatrics (25%), obstetrics/gynecology (19%) and school-based health clinics (8%). Only 3% strongly agreed and 17% agreed that completion of HPV vaccine was more important than completion of pertussis vaccine (Tdap), while 6% strongly agreed and 33% agreed that completion of HPV vaccine was more important than completion of meningitis vaccine (MCV4). Discussion: Providing clinicians with additional information about the cancer prevention benefits of the HPV vaccine and the greater risk for HPV infection/disease relative to other vaccine preventable adolescent diseases may help to increase HPV vaccination rates among adolescents.

The role of human papillomaviruses in cancer progression

The importance of human papillomavirus(HPV)infection and its role in the progress of cancer have been widely evaluated.The understanding of HPV association with certain cancers,such as cervical cancer,is very well established.A big step forward in the prevention of HPV associated cancers with the use of early detection by screening strategies has also been taken.In the last decade,development of HPV vaccination has reduced the number of cases in HPV infections and infection induced cancers.In this report,we review the HPV pathogenesis and highlight the mechanism of HPV involvement in cancer development.

Immune response to plasmid DNA encoding HPV16-L1 protein

Objective To test the immunogenicity of recombinant plasmid DNA containing human papillomavirus type 16 L1 (HPV16 L1) coding sequence of mice Methods The HPV16 L1 encoding sequence was generated by polymerase chain reaction (PCR), and inserted into TA cloning vector PCR Ⅱ, then cloned in the eukaryotic expression vector pcDNA3 1 with CMV promoter The recombinant plasmid DNA pcDNA L1 was transferred into Cos 7 cells and used to immunize BALB/c mice via muscular injection The expression of HPV16 L1 in transferred cells was identified by immunospot and immunocytochemistry, which tested specific anti HPV16 L1 antibody in the serum of immunized mice Results Using the immunospot technique, we found L1 protein expression in pcDNA L1 transferred cells The immunocytochemistry studies demonstrated that the L1 protein was located in nuclei In immunized mice, specific anti HPV16 L1 antibodies could be detected by immunospot and immunocytochemistry 28 days after the first immunization and last at least 41 days Conclusions We constructed HPV16 L1 eukaryotic expressing plasmid whose DNA could induce immuno humoral response in mice This observation will be helpful in designing HPV16 prophylactic vaccine