Study on the Free Radical Scavenging Activity of Sea Cucumber (Paracaudina chinens var.) Gelatin Hydrolysate

Gelatin from the sea cucumber(Paracaudina chinens var.) was hydrolyzed by bromelain and the hydrolysate was found to have a high free radical scavenging activity. The hydrolysate was fractionated through an ultrafiltration membrane with 5 kDa molecular weight cutoff(MWCO). The portion(less than 5 kDa) was further separated by Sephadex G-25. The active peak was col-lected and assayed for free radical scavenging activity. The scavenging rates for superoxide anion radicals(O2·-) and hydroxyl radi-cals(·OH) of the fraction with the highest activity were 29.02% and 75.41%,respectively. A rabbit liver mitochondrial free radical damage model was adopted to study the free radical scavenging activity of the fraction. The results showed that the sea cucumber gelatin hydrolysate can prevent the damage of rabbit liver and mitochondria.

海棒槌胶原蛋白的酶解工艺及其产物清除自由基活性的研究

本文研究了低值的芋参科海参——海棒槌(Paracaudinachinensvar.)的胶原蛋白肽的制备、分离纯化及其清除自由基的活性。利用菠萝蛋白酶对海棒槌胶原进行酶解,通过正交试验,确定菠萝蛋白酶水解海棒槌胶原蛋白的最佳酶解条件为:酶解温度40℃,加酶量240U/ml,底物浓度18mg/100ml(以羟脯氨酸计),pH5.2,酶解时间为4h。在此试验条件下所得产物对超氧阴离子自由基的清除率可达到52.20%。采用超滤和SephadexG-25凝胶柱对酶解液进行分离纯化,得到清除超氧阴离子和羟基自由基能力较强的组分。