Background To study differences among Paragonimus skrjabini (P. skrjabini) populations from five provinces in China ( Guangdong, Fujian, Yunnan, Hubei, and Sichuan) and Paragonimus szechuanensis.Methods DMA sequences ...Background To study differences among Paragonimus skrjabini (P. skrjabini) populations from five provinces in China ( Guangdong, Fujian, Yunnan, Hubei, and Sichuan) and Paragonimus szechuanensis.Methods DMA sequences were obtained from the ITS2 and CO1 genes and phylogenetic trees were constructed from the results. Sequences were also obtained from several other species of Paragonimus for comparative purposes.Results Although differences existed in the DMA sequence among P. skrjabini populations from five provinces, the differences were very small. There was also some resemblance between P. miyazakii from Japan and Fujian strains of P. skrjabini.Conclusion All studied populations can be regarded as different strains of P. skrjabini; P. szechuanensis is not a separate species but may be a geographical strain of P. skrjabini. We also found that P. miyazakii may be synonymous with P. skrjabini.展开更多
Objective To observe triclabendazole effect on Paragonimus skrjabini in experimentally infected rats, and to develop a new drug for treating paragonimiasis.Methods Metacercariae of Paragonimus skrjabini were isolated ...Objective To observe triclabendazole effect on Paragonimus skrjabini in experimentally infected rats, and to develop a new drug for treating paragonimiasis.Methods Metacercariae of Paragonimus skrjabini were isolated from crabs (Sinopotamon) collected from endemic area. Wistar rats were infected intraperitoneally. One and two months after infection, they were treated with triclabendazole at the dosage of 300 mg · kg -1 · 2d-1, 450 mg ·kg -1 · 3d -1 and 600 mg · kg-1 · 3d-1 respectively. Five patients with Paragonimus skrjabini were treated, with Triclabendazole dosage of 10 mg/kg bid x3 days.Results The worm reduction rates were 50. 3%, 80. 8% and 86. 7% respectively one month after completion of treatment. Dead worms of sesame size recovered from muscles, liver, abdominal cavity, chest cavity and lung were greatly diminished in size and weight in comparison with that of the control group. Many large (about 1 cm) black-colored distended worm cysts were found in the lungs of the control rats. Usually there were two adult worms pairs with numerous eggs in each worm cyst. Most worm cysts in the treated groups of rats were changed into hemorrhagic-necrotic patches. All five patients were cured.Conclusion Triclabendazole was highly active against Paragonimus skrjabini in rats experimentally infected and patients.展开更多
基金This study was supported by a NIH/NIAID grant ( No. P50 A139461), USA.
文摘Background To study differences among Paragonimus skrjabini (P. skrjabini) populations from five provinces in China ( Guangdong, Fujian, Yunnan, Hubei, and Sichuan) and Paragonimus szechuanensis.Methods DMA sequences were obtained from the ITS2 and CO1 genes and phylogenetic trees were constructed from the results. Sequences were also obtained from several other species of Paragonimus for comparative purposes.Results Although differences existed in the DMA sequence among P. skrjabini populations from five provinces, the differences were very small. There was also some resemblance between P. miyazakii from Japan and Fujian strains of P. skrjabini.Conclusion All studied populations can be regarded as different strains of P. skrjabini; P. szechuanensis is not a separate species but may be a geographical strain of P. skrjabini. We also found that P. miyazakii may be synonymous with P. skrjabini.
文摘Objective To observe triclabendazole effect on Paragonimus skrjabini in experimentally infected rats, and to develop a new drug for treating paragonimiasis.Methods Metacercariae of Paragonimus skrjabini were isolated from crabs (Sinopotamon) collected from endemic area. Wistar rats were infected intraperitoneally. One and two months after infection, they were treated with triclabendazole at the dosage of 300 mg · kg -1 · 2d-1, 450 mg ·kg -1 · 3d -1 and 600 mg · kg-1 · 3d-1 respectively. Five patients with Paragonimus skrjabini were treated, with Triclabendazole dosage of 10 mg/kg bid x3 days.Results The worm reduction rates were 50. 3%, 80. 8% and 86. 7% respectively one month after completion of treatment. Dead worms of sesame size recovered from muscles, liver, abdominal cavity, chest cavity and lung were greatly diminished in size and weight in comparison with that of the control group. Many large (about 1 cm) black-colored distended worm cysts were found in the lungs of the control rats. Usually there were two adult worms pairs with numerous eggs in each worm cyst. Most worm cysts in the treated groups of rats were changed into hemorrhagic-necrotic patches. All five patients were cured.Conclusion Triclabendazole was highly active against Paragonimus skrjabini in rats experimentally infected and patients.
