S Phase Cell Percentage Normalized BrdU Incorporation Rate, a New Parameter for Determining S Arrest

In this study, a new parameter, S phase cell percentage （S fraction） normalized BrdU （SFN-BrdU） incorporation rate, was introduced to detect $ arrest. The results showed a positive linear correlation between the BrdU incorporation rate and the S fraction in unperturbed 16HBE cells. Theoretical analysis indicated that only S arrest could result in a decrease in the SFN-BrdU incorporation rate. Additionally, the decrease in SFN-BrdU incorporation rate and the activation of DNA damage checkpoints further demonstrated that S arrest was induced by diethyl sulfate treatment of 16HBE cells. In conclusion, $FN-BrdU incorporation rate can be used to detecting S arrest.

Improvement of LCM model and determination of model parameters at watershed scale for flood events in Hongde Basin of China

Considering the fact that the original two-parameter LCM model can only be used to investigate rainfall losses during the runoff period because the initial abstraction is not included, the LCM model was redefined as a three-parameter model, including the initial abstraction coefficient l, the initial abstraction Ia, and the rainfall loss coefficient R. The improved LCM model is superior to the original two-parameter model, which only includes r and R, where r is the initial rainfall loss index and can be calculated with l using the Soil Conservation Service curve number （SCS-CN） method, with r = 1/（1 ＋ λ）. The trial method was used to determine the parameter values of the improved LCM model at the watershed scale for 15 flood events in the Hongde Basin in China. The results show that larger r values are associated with smaller R values, and the parameter R ranges widely from 0.5 to 2.0. In order to improve the practicability of the LCM model, r = 0.833 with λ = 0.2 is reasonable for simplifying calculation. When the LCM model is applied to arid and semi-arid regions, rainfall without yielding runoff should be deducted from the total rainfall for more accurate estimation of rainfall-runoff.

Rheological Behaviour for Polymer Melts and Concentrated SolutionsPart Ⅱ: Material Function with Nagai Chain Constraints and Determination of Their Parameters from Flow Curves

An integral constitutive equation and a set of material functions for describing the strain history of polymer melts were formulated in terms of the Cauchy-Green and Finger tensors. A simple memory function and the dependence of ηo and τt on M3.4 were derived from the theory of non-linear viscoelasticity with constraints of entanglements for polymer melts and substituted into the Oldroye-Walters-Fredickson constitutive equation. An integral constitutive equation for polymer melts was consequently obtained. Some material functions of the constitutive equation related to certain 'test flow' are examined as follows : (1) simple steady shear flow; (2) steady elongation flow; (3) small-amplitude oscillatory shear flow; (4) stress growth upon the inception of steady shear elongation flow; (5) stress relaxation (modulus and compllance). These theoretical relations for simple steady shear flow were compared with experimental data from our laboratory and references for various polymer melts and concentrated solutions. A good agreement between the theory and experiment was achieved.

DETERMINATION OF CREEP PARAMETERS FROM INDENTATION CREEP EXPERIMENTS

The possibilities of determining creep parameters for a simple Norton law material are explored from indentation creep testing. Using creep finite element analysis the creep indentation test technique is analyzed in terms of indentation rates at constant loads. Emphasis is placed on the relationships between the steady creep behavior of indentation systems and the creep property of the indented materials. The role of indenter geometry, size effects and macroscopic constraints is explicitly considered on indentation creep experiments. The influence of macroscopic constraints from the material systems becomes important when the size of the indenter is of the same order of magnitude as the size of the testing material. Two methods have been presented to assess the creep property of the indented material from the indentation experimental results on the single-phase-material and two-phase-material systems. The results contribute to a better mechanical understanding and extending the application of indentation creep testing.

Identification of the Discrete Element Model Parameters for Rock-Like Brittle Materials

An inverse method for parameters identification of discrete element model combined with experiment is proposed.The inverse problem of parameter identification is transmitted to solve an optimization problem by minimizing the distance between the numerical calculations and experiment responses.In this method,the discrete element method is employed as numerical calculator for the forward problem.Then,the orthogonal experiment design with range analysis was used to carry out parameters sensitivity analysis.In addition,to improve the computational efficiency,the approximate model technique is used to replace the actual computational model.The intergeneration projection genetic algorithm(IP-GA)is employed as the optimization algorithm.Consequently,the parameters of the discrete element model are determined.To verify the effectiveness and accuracy of the inverse results,the comparisons of shape deviation experiments with discrete element simulations are provided.It indicates that the effective and reliable discrete element model parameters can be quickly obtained through several sets of experimental data.Hence,this inverse method can be applied more widely to determine the parameters of discrete element model for other materials.

Experimental study on parameter determination of fluid replacement equation for volcanic reservoirs

In this study,the parameters of Gassmann equation based on fluid replacement theory are studied by measuring the acoustic velocity during the evaporation process of volcanic rocks in Nanpu area.The experimental data show that with the decrease of porosity of tight volcanic rock,the acoustic velocity difference between dry and wet rock samples increases,which is conducive for the identification of gas bearing reservoirs with acoustic log data.The fluid bulk modulus distribution of volcanic rocks in the study area conforms to Brie model,and the value of empirical coefficient e is related to lithology.The experimental results show that there is a linear relationship between the P-wave transit time of dry and wet rock samples.Using porosity to calculate the acoustic transit time of saturated rock samples,and taking it into the experimental formula,we can get the P-wave transit time and bulk modulus of dry rock samples.According to the bulk modulus of mixed fluid,dry rock and rock matrix determined by experiments,the saturation of volcanic reservoir in Nanpu area is calculated by Gassmann equation,which is in good contrast with the conclusion of gas test.This study provides an experimental basis for quantitative evaluation of volcanic gas reservoirs using seismic and acoustic logging data.

CHRONOABSORPTOMETRY FOR THE DETERMINATION OF KINETIC PARAMETERS OF ELECTRON TRANSFER REACTIONS USING LONG-OPTICAL-PATH ELECTROCHEMICAL CELL

A single potential step chronoabsorptometric method for the determination of ki- netic parameters of simple quasi-reversible reactions is described.It is verified by determining the kinetic parameters for the electroreduction of ferricyanide.A long-optical-path electro- chemical cell with a plug-in electrode is used.The thickness of solution layer is 0.55 mm

Research and Application for the Regularity of Distribution about Curvature Radius and Curvature Center of the Linkage Point Track in a Link Mechanism

It is illustrated that there exists an inflection circle on the linkage rigid body by the principle of relative motion. Confirmed methods of the inflection circle, curvature radius and curvature center of the point track on the linkage rigid body are given in the case of the different contact type of move instantaneous center line and static instantaneous center line. The regularity of distribution of curvature radius and curvature center of the point track is researched. The identification methods called determination parameters and auxiliary vertical line of the diameter and direction of the inflection circle in the four bar mechanism are pointed out. A design method of the crane hoisting mechanism is discussed in the end of this paper.

DETERMINATION OF CREEP PROPERTIES OF THERMAL BARRIER COATING(TBC)SYSTEMS FROM THE INDENTATION CREEP TESTING WITH ROUND FLAT INDENTERS

Indentation creep behavior with cylindrical flat indenters on the thermal barrier coating (TBC) was studied by finite element method (FEM). On ike constant applied indentation creep stress, there is a steady creep rate for each case studied for different creep properties of the TBC system. The steady creep depth rate depends on the applied indentation creep stress and size of the indenters as well as the creep properties of the bond coat of the TBC and the substrate. The possibilities to determine the creep properties of a thermal barrier system from indention creep testing were discussed. As an example, with two different size indenters, the creep properties of bond coat of the TBC system can be derived by an inverse FEM method. This study not only provides a numerical method to obtain the creep properties of the TBC system, but also extends the application of indentation creep method with cylindrical flat indenters.

A Study on the Dynamic Adjustment of Pressure Relief Gas Drainage Drilling in Mined-Out Areas

With the development of coal mine equipment mechanization, the wide application of “hole instead of roadway” technology greatly reduces the cost of gas control engineering, but puts forward higher requirements for the effect of gas drainage. At present, the drainage effect of coal mine inspection boreholes is mainly evaluated by the drilling field, but the flow rate and gas concentration of each borehole in the drilling field are not the same, which causes the gas drainage effect not to be correctly mastered. In the present study, the pressure relief drilling in the goaf of the working face of a typical multi-coal seam group high gas outburst mining area was taken as the research object. Through the newly developed portable drilling inspection device, the pure amount of drilling drainage was investigated, and the drilling design was dynamically adjusted. The enhancement of the goaf pressure relief gas control effect ensures the gas safety of the mining face. At the same time, this improves the gas extraction rate and reduces the emission of greenhouse gases. If the data from the borehole investigation can be transmitted in real time and analyzed in big data, the optimal extraction negative pressure can be predicted through a regression algorithm. Under the control of the negative pressure of each borehole by the actuator, the extraction system can have the function of intelligent judgment.

A Simple Application and Design of Genetic Algorithm in Card Problem

According to traditional card problem solving which is based on the idea of genetic algorithm(GA),a set of algorithms is designed to find final solution.For each process in genetic algorithm,including choices of fitness function,parameters determination and coding scheme selection,classic algorithm is used to realize the various steps,and ultimately to find solution of problems.

Towards combining multiple geophysical datasets to determine earthquake source parameters in China

1.Difficulties of conventional seismic studies on earthquake source parameters Earthquake source parameters,including magnitude,location,focal mechanism,rupture process are key factors for understanding seismogenic environment,mitigating seismic hazards,estimating earthquake triggering,and tectonic analysis.Traditionally,source parameters are determined by seismological methods.For example,Fang L H et al.(2014)relocated the 2012 Ms6.6 Xinjiang Xinyuan earthquake sequence using local seismograms based on the double difference method,and obtained the distribution of

A simplified method for the determination of vertically loaded pile-soil interface parameters in layered soil based on FLAC3D

The numerical analysis of pile-soil interaction commonly requires a lot of trial works to determine the interface parameters and the accuracy cannot be ensured normally. Considering this, this paper first conducts a sensitivity analysis to figure out the influence of interface parameters on the bearing behavior of a single pile in sand. Then, a simplified method for the determination of pile-soil interface parameters in layered soil is proposed based on the parameter studies. Finally, a filed loading test is used for the validation of the simplified method, and the calculated results agree well with the monitoring data. In general, the simplified method proposed in this paper works with higher accuracy and consumes less time compared with the traditional trial works, especially on the determinations ofinterfacial cohesive and interracial friction angle.

Determination of HMO parameters with MS-Xα-SCF method

A technique for determining the HMO parameters with MS-Xα-SCF method has been de- veloped. The ionization potentials of even polyenes and condensed benzene compounds have been calculated. The average deviation from the existent experimental values is 0.34 eV.

Derivative-extremum analysis of current-potential curves showing electrochemical kinetics in the full reversibility range

Derivative-extremum analysis(DEA) of j-E curves is a newly proposed method of half wave potential(E1/2) and activation feature extraction from steady-state voltammetry. Here, the DEA is demonstrated to be valid in the full range of reversibility using numerical simulations with a derived universal electrode equation, providing a novel perspective of electrochemical kinetics in the reversibility domain. The results reveal that E1/2is a better choice of the reference potential instead of equilibrium potential(Eeq) in electrode equations, especially since Eeqis meaningless in an irreversible case. The equations referenced with standard potential, E1/2and Eeq, are summarized in three tables, and their applications in parameter determinations are specified. Finally, reversibility is proved to be a relative measure between kinetic slowness and mass transport of electroactive species, and the reversibility classifications are proposed according to the DEA feature in the reversibility domain. This work, based on the DEA principle, refines the electrode equation forms and generalizes their applicability in the full range of reversibility.

Intrusion Detection Model with Twin Support Vector Machines

Intrusion detection system(IDS) is becoming a critical component of network security. However,the performance of many proposed intelligent intrusion detection models is still not competent to be applied to real network security. This paper aims to explore a novel and effective approach to significantly improve the performance of IDS. An intrusion detection model with twin support vector machines(TWSVMs) is proposed.In this model, an efficient algorithm is also proposed to determine the parameter of TWSVMs. The performance of the proposed intrusion detection model is evaluated with KDD'99 dataset and is compared with those of some recent intrusion detection models. The results demonstrate that the proposed intrusion detection model achieves remarkable improvement in intrusion detection rate and more balanced performance on each type of attacks.Moreover, TWSVMs consume much less training time than standard support vector machines(SVMs).

Establishment and verification of a model for the movement of pulverized sweet sorghum stalks in a rotary drum bioreactor by discrete element method

The mixing of raw materials in a rotary drum bioreactor is important for advanced solid-state fermentation technology.However,the shape,size,and other properties of pulverized sweet sorghum stalk particles are more complicated than those of the spherical particles.In this study,a soft rod-shaped discrete particle model was established and verified to simulate the mixing behavior of sweet sorghum stalk particles in a rotary drum bioreactor.We were inspired by the particle shape and established a rod-shaped particle model by investigating the influence of the shape(length-diameter ratio)and size(diameter)on the particle packing(stack height and bed porosity).We used orthogonal simulations and extremum difference analysis to determine the main factors,optimum level,and groups of other parameters.Based on calibrated parameters,twelve sets of simulations of radial mixing in the drum were performed,and the results were compared with experiments conducted under identical operating conditions.The average relative error between the simulation and the experiment was 10.95%,which indicates that they agreed well and that the simulation could predict the mixing process well.