Determination of 10 Kinds of Inorganic Elements in Paris polyphylla Smith var. yunnanensis(Franch.) Hand.-Mazz.(and Polygerm Varieties)

[Objectives] To determine the content of 10 kinds of inorganic elements(Pb,Cu,As,Cd,Hg,Zn,Fe,Mn,Sr and Cr) in samples of Paris polyphylla Smith var. yunnanensis( Franch.) Hand.-Mazz.( and polygerm varieties) produced in different areas. [Methods] The wet digestion and technique of inductively coupled plasma optical emission spectrometry( ICP-OES) were used. [Results] Under the experimental conditions,elements were not related to each other,and several kinds of elements could be measured at the same time. The content of Pb exceeding the standard only in sample S4 of P. polyphylla Smith var. yunnanensis( and polygerm varieties),Hg was not detected in all samples,and the rest heavy metals did not exceed the limit. [Conclusions] This study was expected to provide a certain reference for the development and utilization of P. polyphylla Smith var. yunnanensis( and polygerm varieties).

Identification of Paris polyphylla Smith var. yunnanensis(Franch.) Hand.-Mazz.(Polygerm Varieties) Produced in Gaoligong Mountain Areas

[Objectives] To make original plant and microscopic identification and study the traits of Paris polyphylla Smith var. yunnanensis( Franch.) Hand.-Mazz.( polygerm varieties) produced in Gaoligong Mountain areas,and provide experimental data for their identification and application. [Methods] The original plant powder was used,and paraffin sections and physicochemical properties were identified. [Results]P. polyphylla Smith var. yunnanensis( polygerm varieties) produced in Gaoligong Mountain areas showed obvious microscopic features and physicochemical properties. [Conclusions] This study was expected to provide certain reference for identification of original plant and medicine and formulation of quality standard for P. polyphylla Smith var. yunnanensis( polygerm varieties) produced in Gaoligong Mountain areas,and provided basis for their further research and development.

A novel phenylpropanoid glycosides and a new derivation of phenolic glycoside from Paris Polyphylla var. yunnanensis

A novel phenylpropanoid glycosides 1, named parispolyside E and a novel derivation of phenolic glycoside 2, named parispolyside G, as well as two known flavonoid glycosides were isolated from the rhizome of Paris polyphylla var. yunnanensis. Their structures were elucidaed by spectroscopic methods.

Isolation of the Endophytic Fungi of Paris polyphylla var. yunnanensis and Their Effects on the Embryo Development of P. polyphylla var. yunnanensis Seeds

[Objective] This study aimed to isolate the endophytic fungi of Paris polyphylla var. yunnanensis and investigate their effects on the embryo development of P. polyphylla var. yunnanensis seeds. [Method] The endophytic fungi of P. polyphylla were isolated and identified morphologically, and their effects on the embryo development of P. polyphylla var. yunnanensis seeds were studied by using paraffin sectioning and microphotography. [Result] Nine endophytic fungi, i.e. P. polyphylla var. yunnanensis endophytic fungi PPYEF-1, PPYEF-2, PPYEF-3, PPYEF-4, PPYEF-5, PPYEF-6, PPYEF-7, PPYEF-8 and PPYEF-9 belonging to seven genera in five families, three orders were isolated from the rhizomes. Except PPYEF-4 (Cladosporium sp.), other fungi could promote the embryo development of the P. polyphylla var. yunnanensis seeds, mostly reaching the extremely significant or significant level. PPYEF-9 (Trichoderma sp.) resulted in the highest embryo length and embryo-emerging ratio. [Conclusion] This paper could provide a reference for the application of the endophytic fungi of P. polyphylla var. yunnanensis in the dormancy-breaking of P. polyphylla var. yunnanensis seeds.

Levels of rhizome endophytic fungi fluctuate in Paris polyphylla var.yunnanensis as plants age

Paris polyphylla var. yunnanensis is an important medicinal plant with abundant saponins that are widely used in the pharmaceuticals industry. It is unclear why the levels of active ingredients increase as these plants age. We speculated that the concentrations of those components in the rhizomes are mediated by fungal endophytes. To test this hypothesis, we took both culture-dependent and-independent(metagenomics) approaches to analyze the communities of endophytic fungi that inhabit those rhizomes in plants of different age classes(four, six, and eight years old). In all, 147 isolates representing 18 fungal taxa were obtained from 270 segments(90 per age class). Based on morphological and genetic characteristics, Fusarium oxysporum(46.55% frequency of occurrence) was the predominant endophyte,followed by Leptodontidium sp.(8.66%) and Trichoderma viride(6.81%). Colonization of endophytic fungi was maximized in the eight-year-old rhizomes(33.33%) when compared with four-year-old(21.21%) and six-year-old(15.15%) rhizomes. Certain fungal species were present only at particular ages. For example,Alternaria sp., Cylindrocarpon sp., Chaetomium sp., Paraphaeosphaeria sporulosa, Pyrenochaeta sp., Penicillium swiecickii, T. viride, and Truncatella angustata were found only in the oldest plants. Analysis of(metagenomics) community DNA extracted from different-aged samples revealed that, at the class level,the majority of fungi had the highest sequence similarity to members of Sordariomycetes, followed by Eurotiomycetes and Saccharomycetes. These results were mostly in accord with those we obtained using culture methods. Fungal diversity and richness also changed over time. Our investigation is the first to show that the diversity of fungi in rhizomes of P. polyphylla var. yunnanensis is altered as plants age, and our findings provide a foundation for future examinations of useful compounds.

Mycorrhizas Affect Polyphyllin Accumulation of Paris polyphylla var.yunnanensis through Promoting PpSE Expression

Paris polyphylla var.yunnanensis is a traditional Chinese medicinal plant,in which polyphyllin as the main medicinal component is an important secondary metabolite with bioactivity.Arbuscular mycorrhizal fungi(AMF)have multiple positive effects on plants,while it is not clear whether AMF increase the content of medicinal components in medicinal plants.In this study,a total of nine AMF treatments were laid to analyze the mycorrhizal effect on polyphyllin accumulation and PpHMGR and PpSE expression of P.polyphylla var.yunnanensis.AMF increased the content of polyphyllin in the cultivated variety with low relation to the increase of inoculation intensity.Polyphyllin I,II,and VII were identified and partly improved by AMF inoculation,dependent on AMF treatments and culture environments.Similarly,the PpHMGR and PpSE expression was induced by mycorrhization,dependent on AMF species,whilst the induction was more obvious in PpSE than in PpHMGR after mycorrhization.It concluded that the symbiotic relationship between P.polyphylla var.yunnanensis and AMF increased polyphyllin content level in the plant,which was associated with the up-regulation of PpSE transcripts.

Mycorrhizal Fungal Effects on Growth,Antioxidant Capacity,and Medicine Quality of Paris polyphylla var.yunnanensis

A field experiment was conducted to determine the effects of two commercial strains composed of mulple arbuscular mycorrhizal fungi(AMF)species on plant growth,antioxidant capacity,and medicine quality of Paris polyphylla var.yunnanensis in three subtropical soils from Wanzhou,Anshun and Baoshan in fields.The results showed that AMF inoculation enhanced the fungal colonization rate and activities of both succinate dehydrogenase and alkaline phosphatase,thereby,enhancing the mycorrhizal viability of P.polyphylla var.yunnanensis.The concentrations of photosynthetic pigments(chlorophyll a,b,a+b and carotenoids),soluble sugar,soluble protein and photosynthetic capacity were higher in AMF-inoculated plants than in non-AMF-treated plants in field.AMFtreated plants recorded higher activities of catalase,peroxidase and superoxide dismutase,and caused the reduction in malondialdehyde content,indicating lower oxidative damage,compared with non-AMF plants.Polyphyllin I,Polyphyllin II,Polyphyllin III,Polyphyllin IV and total polyphyllin contents were increased by AMF treatment.In conclusion,AMF improved the plant growth,antioxidant capacity and medicinal quality of P.polyphylla var.yunnanensis seedlings.Hereinto,AMF effects on the soil from Wanzhou was relatively greater than on other soils.

Epigenetic Factors Altered in Dehisced Anther Correlated to Seed Dormancy in <i>Paris polyphylla</i>var. Yunnanensis

Paris polyphylla var. yunnanensis (Franch.), one of the best-known medicinal plants in China, has a dehiscent anther which physiologically work in pollination, however, the dehiscent anther always closes in response to darkness every day, and watering or raining every time. To explore this frequently closing and its unkown physiology, next-generation sequencing was performed, and the transcriptome was de novo assembled. RNA-sequencing was carried out in 15 samples including seven openning samples, four closed samples owing to darkness or watering, and tissue samples (leaf, petal, calyx, and stigma) were used for control. We obtained 72.75 GB data, assembled into 79,815 unigenes. Differentially expressed unigenes (DEGs) between opened and closed anther samples were 6231 and the DEGs between anther and control samples were 2831. Comparation between the two DEGs by KEGG enrichment showed that “plant hormone signal transduction” pathway is the most significant pathway for DEGs from closing anther vs. opening anther, and expression model of DEGs in the pathway might elicit change in germination and seed dormancy. Further examination of the action of the signal pathway on physiology showed “chromatin binding” function was prominent in “DNA binding” function of annotated DEGs between opened and closed anthers, of the 215 “chromatin binding” unigenes, 120 were involved in epigenetic silencing, and 50 of the epigenetic unigenes were directly related to germination or seed dormancy, strongly correlating anther closing to epigenetic modification and seed dormancy. These results were verified that at least three auxins involved in seed dormancy showed same expression patterns occurred in abnormal closing anther and seed embryo in Paris polyphylla var. yunnanensis. In conclusion, the information from transcriptome point out that frequent abnormal closing of dehiscent anthers possibly transfer the impact on seed dormancy, and epigenetic modification happened in closing may be the cause.

Investigation on the Resources of Paris Polyphylla var.yunnanensis

In this study,the resource distribution,morphological characteristics,artificial cultivation and market circulation of Paris Polyphylla var.yunnanensis and its polygerm varieties in Yunnan were investigated by referring to the research literature,field investigation and our planting experience.It is found that P.polyphylla var.yunnanensis is widely distributed in Yunnan Province,realizing large-scale artificial cultivation,while the wild resources of polygerm varieties are almost on the verge of extinction and are in urgent need of protection.

Parisaponin Ⅰ和Parisvietnaside A的NMR数据分析

从滇重楼中分离得到2个甾体皂苷,利用1H NMR、13C NMR、1H-1H COSY、HSQC、HMBC、1D和2D TOCSY等多种核磁共振方法鉴定其结构分别为(25R)-26-O-β-D-吡喃葡萄糖基-3β,22α,26-三羟基-呋甾-5-烯-3-O-α-L-吡喃鼠李糖基-(1→2)-[α-L-呋喃阿拉伯糖基-(1→4)]-β-D-吡喃葡萄糖苷(1,Parisaponin I)和(25R)-3β,5α,6β-三羟基-△7-螺甾烯-3-O-β-D-吡喃葡萄糖基-(1→3)-[α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷(2,paris-vietnaside A).对化合物1和2的1H NMR和13C NMR信号分别进行了归属和详细分析,并纠正了文献中的核磁数据归属错误.化合物2为首次从滇重楼中分离得到.

Unraveling the serial glycosylation in the biosynthesis of steroidal saponins in the medicinal plant Paris polyphylla and their antifungal action

Sugar-sugar glycosyltransferases play important roles in constructing complex and bioactive saponins.Here,we characterized a series of UDP-glycosyltransferases responsible for biosynthesizing the branched sugar chain of bioactive steroidal saponins from a widely known medicinal plant Paris polyphylla var.yunnanensis.Among them,a 2'-O-rhamnosyltransferase and three 6'-O-glucosyltrasferases catalyzed a cascade of glycosylation to produce steroidal diglycosides and triglycosides,respectively.These UDP-glycosyltransferases showed astonishing substrate promiscuity,resulting in the generation of a panel of 24 terpenoid glycosides including 15 previously undescribed compounds.A mutant library containing 44 variants was constructed based on the identification of critical residues by molecular docking simulations and protein model alignments,and a mutant UGT91AH1^(Y187A)with increased catalytic efficiency was obtained.The steroidal saponins exhibited remarkable antifungal activity against four widespread strains of human pathogenic fungi attributed to ergosterol-dependent damage of fungal cell membranes,and 2'-O-rhamnosylation appeared to correlate with strong antifungal effects.The findings elucidated the biosynthetic machinery for their production of steroidal saponins and revealed their potential as new antifungal agents.

云南重楼种子育苗技术研究

目的：缩短云南重楼种子萌发和出苗所需时间，提高其出苗率；提出在生产中切实可行的云南重楼种子有性繁殖育苗方法。方法：考察不同温度、不同播种方式（播种时间、覆土厚度、地膜覆盖）等因素对云南重楼种子萌发和出苗的影响。结果：云南重楼种子胚后熟和萌发的最适温度在18～20℃，在此温度下处理3～4个月以后，转至0～10℃低温处理2～4个月，可在翌年4月出苗；适宜的播种时间是4月份以前，育苗土覆盖厚度为1cm左右，播种后用黑色地膜进行覆盖，可提高出苗率。结论：上述试验结果可为云南重楼种子育苗提供操作参考。

滇重楼的化学成分研究

目的研究滇重楼Paris polyphylla var.yunnanensis根茎中的化学成分。方法利用正相硅胶柱色谱、葡聚糖凝胶Sephadex LH-20、反相制备色谱等手段进行分离纯化,并通过1H-NMR、13C-NMR、ESI-MS等波谱技术进行结构鉴定。结果从醋酸乙酯层中分离得到了7个化合物,分别鉴定为豆甾醇-3-O-β-D-葡萄糖苷(Ⅰ)、1-O-(β-D-葡萄糖基)-(2S,3S,4E,8E)-2-[(2′R)-2′-羟基十六酰氨基]-4(E),8(E)-十八二烯-1,3-二醇(Ⅱ)、β-蜕皮激素(Ⅲ)、薯蓣皂苷元-3-O-α-L-呋喃阿拉伯糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-葡萄糖苷(Ⅳ)、薯蓣皂苷元-3-O-α-L-吡喃鼠李糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-葡萄糖苷(Ⅴ)、薯蓣皂苷元-3-O-α-L-吡喃鼠李糖基(1→4)-α-L-吡喃鼠李糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-葡萄糖苷(Ⅵ)和偏诺皂苷元-3-O-α-L-呋喃阿拉伯糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-葡萄糖苷(Ⅶ)。结论化合物Ⅱ为首次从该属植物中分离得到。

云南重楼高产栽培施肥研究

为探明肥料施用对云南重楼产量的影响，采用不同梯度施肥水平研究有机肥对云南重楼产量的影响；采用三元二次回归正交设计方法研究NPK无机肥对云南重楼产量的影响。结果表明，云南重楼的高产需要较高的肥力条件，在中等以下的土壤肥力条件下，云南重楼的最佳农家肥施肥量为3000～4500kg/667m2之问。N素和P素是云南重楼生长的养分限制因子。可见，施用适宜有机肥和N、P无机肥可以大幅度提高云南重楼单位面积产量。

超声波对滇重楼种子萌发能力的影响

研究超声波处理时间、温度、功率对滇重楼(Paris polyphylla var.yunnanensis)种子萌发的影响,寻找其萌发的最佳条件。通过室内常规发芽试验,统计滇重楼种子在不同超声波处理时间、温度、功率条件下的发芽率和发芽指数。结果表明,滇重楼种子经适当的超声波处理,缩短了萌发时间,提高了发芽率和发芽指数。40 min、50℃、200 W的超声波处理条件最适宜于滇重楼种子萌发。

云南重楼种子萌发过程内源激素含量及酶活性变化研究

为探讨云南重楼种子萌发不同阶段的酶和内源激素的变化,本研究采用生理生化方法测定云南重楼种子萌发不同阶段超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、苹果酸脱氧酶(MDH)和6-磷酸葡萄糖脱氢酶(G-6-PDH)活性,以及吲哚乙酸(IAA)、赤霉素(GA_3)、脱落酸(ABA)、水杨酸(SA)、反玉米素(TZ)、茉莉酸(JA)6种内源激素的含量,并分析比较其差异。结果表明,云南重楼种子在即将出苗阶段的SOD、POD活性迅速升高,CAT、MDH和G-6-PDH活性降低;IAA、GA_3含量均在即将出苗阶段显著高于其他阶段,种子休眠解除后ABA含量迅速下降。胚根刚突破种皮阶段,其SA、TZ、JA含量均最高,SOD、POD、IAA、GA_3间呈极显著正相关(P<0.01),MDH与G-6-PDH呈极显著正相关(P<0.01),ABA与GA_3呈显著负相关(P<0.05),SA、TZ、JA间呈极显著正相关(P<0.01),IAA/ABA、GA_3/ABA、SA/ABA在种子休眠解除和出苗阶段均呈上升趋势。综上,SOD、POD、GA_3是种子出苗阶段的关键物质,TZ、SA和JA对种子休眠解除具有重要的影响,IAA/ABA、GA_3/ABA及SA/ABA增大对种子休眠解除和出苗均有促进作用。本研究结果为云南重楼种子繁殖提供了理论依据和实践指导。

滇重楼内生真菌的生物学性状及其甾体化合物含量

从滇重楼根状茎中分离到133株内生真菌,对其中的7株真菌进行了生物学性状观察和甾体化合物含量测定。结果表明,2号、8号和12号真菌能合成大量的甾体化合物,培养液中甾体化合物的产率分别为208.62,144.97和198.79 mg/L。

不同海拔、土壤类型及肥力对云南重楼产量和质量的影响研究

研究了不同海拔高度、土壤类型及肥力条件下云南重楼的生长情况。结果表明,在1700～3100 m海拔范围内,云南重楼的产量与海拔高度没有相关关系,但与土壤的类型、肥力和耕作水平有关系;云南重楼在经常翻用、有机质含量或速效肥力较高的壤土中生长可获得较高的产量。不同海拔、土壤类型及肥力对云南重楼质量没有影响。

AM真菌对滇重楼根际土壤微生物数量及酶活性的影响

目的:研究接种AM真菌后滇重楼根际土壤微生物和酶活性的变化规律,为人工栽培滇重楼提供技术基础。方法:通过室温盆栽接种试验与室内分析相结合的方法,观察灭菌土壤条件下接种28种丛枝菌根(AM)真菌对滇重楼根际土壤微生物和土壤酶活性的影响。结果:滇重楼与AM真菌之间存在一定的相互选择性。AM真菌诱导处理后,不同AM真菌对滇重楼根际土壤微生物的数量、微生物生物量碳和土壤酶活性的影响有所差异,AM真菌的施用降低了滇重楼根际土壤中可培养真菌的数量,而对可培养细菌、放线菌数量呈现出增加的趋势,并提高了根际土壤微生物多样性指数、微生物生物量碳含量以及蛋白酶、脲酶、磷酸酶、过氧化氢酶和蔗糖酶活性。结论:滇重楼人工栽培中施用AM真菌能促使滇重楼根际微生物区系从低肥力的"真菌型"向高肥力的"细菌型"转化的趋势,提高根际土壤微生物多样性和酶活性,有助于维持滇重楼根际微生态系统的稳定性与和谐性,证明了田间人工栽培滇重楼施加AM菌剂的有效性及可能性。

氮、磷、钾对滇重楼产量及皂苷含量的影响

滇重楼是名贵道地中药材,过度采挖已使其野生资源濒临灭绝,人工栽培是解决滇重楼资源危机的必然途径。合理施用氮、磷、钾肥可提高其药用部位产量和品质。本文通过"3414"田间小区肥料效应试验,设N:23 kg/667 m^2、P2O5:12 kg/667 m^2、K2O:30 kg/667 m^2为常规施肥水平,研究不同水平氮、磷、钾对滇重楼产量及皂苷含量的影响。结果表明:土壤氮、磷、钾的贡献率分别为77.97%,64.93%和136.52%,土壤供钾量丰富,供氮量中等,供磷量不足;增施氮肥和磷肥,不施钾肥可提高滇重楼的产量。由滇重楼根茎增产率的单因素、双因素和三因素肥料效应函数方程可知:单施氮、磷、钾肥对滇重楼根茎增产率影响较小,氮、磷、钾配施对滇重楼根茎增产率影响最大;三因素肥料效应方程推荐的最佳施肥量(N:34.5 kg/667 m^2,P2O5:18 kg/667 m^2,K2O:0 kg/667 m^2)所得滇重楼根茎增长率最高,为87%;适量的氮肥和磷肥可提高滇重楼新、老根茎的皂苷含量,增施钾肥会使滇重楼新根茎皂苷含量降低。当氮肥和磷肥施用量分别为23 kg/667 m^2和18 kg/667 m^2时,滇重楼新、老根茎总皂苷含量最高;钾肥施用量为0 kg/667 m^2时,滇重楼新根茎总皂苷含量最高。