期刊文献+
共找到126篇文章
< 1 2 7 >
每页显示 20 50 100
A novel phenylpropanoid glycosides and a new derivation of phenolic glycoside from Paris Polyphylla var. yunnanensis 被引量:7
1
作者 Yu Wang Wen Yuan Gao +1 位作者 Tie Jun Zhang Yuan Qiang Guo 《Chinese Chemical Letters》 SCIE CAS CSCD 2007年第5期548-550,共3页
A novel phenylpropanoid glycosides 1, named parispolyside E and a novel derivation of phenolic glycoside 2, named parispolyside G, as well as two known flavonoid glycosides were isolated from the rhizome of Paris poly... A novel phenylpropanoid glycosides 1, named parispolyside E and a novel derivation of phenolic glycoside 2, named parispolyside G, as well as two known flavonoid glycosides were isolated from the rhizome of Paris polyphylla var. yunnanensis. Their structures were elucidaed by spectroscopic methods. 展开更多
关键词 paris polyphylla var. yunnanensis Phenylpropanoid glycosides Phenolic glycoside parispolyside F parispolyside G
下载PDF
Isolation of the Endophytic Fungi of Paris polyphylla var. yunnanensis and Their Effects on the Embryo Development of P. polyphylla var. yunnanensis Seeds 被引量:4
2
作者 程坤 杨艳琼 +4 位作者 赵昶灵 王荔 陈疏影 申毓晗 柴静 《Agricultural Science & Technology》 CAS 2012年第7期1497-1501,1570,共6页
[Objective] This study aimed to isolate the endophytic fungi of Paris polyphylla var. yunnanensis and investigate their effects on the embryo development of P. polyphylla var. yunnanensis seeds. [Method] The endophyti... [Objective] This study aimed to isolate the endophytic fungi of Paris polyphylla var. yunnanensis and investigate their effects on the embryo development of P. polyphylla var. yunnanensis seeds. [Method] The endophytic fungi of P. polyphylla were isolated and identified morphologically, and their effects on the embryo development of P. polyphylla var. yunnanensis seeds were studied by using paraffin sectioning and microphotography. [Result] Nine endophytic fungi, i.e. P. polyphylla var. yunnanensis endophytic fungi PPYEF-1, PPYEF-2, PPYEF-3, PPYEF-4, PPYEF-5, PPYEF-6, PPYEF-7, PPYEF-8 and PPYEF-9 belonging to seven genera in five families, three orders were isolated from the rhizomes. Except PPYEF-4 (Cladosporium sp.), other fungi could promote the embryo development of the P. polyphylla var. yunnanensis seeds, mostly reaching the extremely significant or significant level. PPYEF-9 (Trichoderma sp.) resulted in the highest embryo length and embryo-emerging ratio. [Conclusion] This paper could provide a reference for the application of the endophytic fungi of P. polyphylla var. yunnanensis in the dormancy-breaking of P. polyphylla var. yunnanensis seeds. 展开更多
关键词 paris polyphylla var. yunnanensis Endophytic fungus P. polyphylla var. yunnanensis seed Embryo development EMBRYO
下载PDF
Levels of rhizome endophytic fungi fluctuate in Paris polyphylla var.yunnanensis as plants age 被引量:4
3
作者 Tao Liu Alex Greenslade ShengchaoYang 《Plant Diversity》 SCIE CAS CSCD 北大核心 2017年第1期60-64,共5页
Paris polyphylla var. yunnanensis is an important medicinal plant with abundant saponins that are widely used in the pharmaceuticals industry. It is unclear why the levels of active ingredients increase as these plant... Paris polyphylla var. yunnanensis is an important medicinal plant with abundant saponins that are widely used in the pharmaceuticals industry. It is unclear why the levels of active ingredients increase as these plants age. We speculated that the concentrations of those components in the rhizomes are mediated by fungal endophytes. To test this hypothesis, we took both culture-dependent and-independent(metagenomics) approaches to analyze the communities of endophytic fungi that inhabit those rhizomes in plants of different age classes(four, six, and eight years old). In all, 147 isolates representing 18 fungal taxa were obtained from 270 segments(90 per age class). Based on morphological and genetic characteristics, Fusarium oxysporum(46.55% frequency of occurrence) was the predominant endophyte,followed by Leptodontidium sp.(8.66%) and Trichoderma viride(6.81%). Colonization of endophytic fungi was maximized in the eight-year-old rhizomes(33.33%) when compared with four-year-old(21.21%) and six-year-old(15.15%) rhizomes. Certain fungal species were present only at particular ages. For example,Alternaria sp., Cylindrocarpon sp., Chaetomium sp., Paraphaeosphaeria sporulosa, Pyrenochaeta sp., Penicillium swiecickii, T. viride, and Truncatella angustata were found only in the oldest plants. Analysis of(metagenomics) community DNA extracted from different-aged samples revealed that, at the class level,the majority of fungi had the highest sequence similarity to members of Sordariomycetes, followed by Eurotiomycetes and Saccharomycetes. These results were mostly in accord with those we obtained using culture methods. Fungal diversity and richness also changed over time. Our investigation is the first to show that the diversity of fungi in rhizomes of P. polyphylla var. yunnanensis is altered as plants age, and our findings provide a foundation for future examinations of useful compounds. 展开更多
关键词 Medicinal plants Endophytic fungi paris polyphylla var.yunnanensis
下载PDF
Mycorrhizas Affect Polyphyllin Accumulation of Paris polyphylla var.yunnanensis through Promoting PpSE Expression 被引量:3
4
作者 Hailing Li Lingfeng Xu +4 位作者 Zhuowei Li Shunxin Zhao Dongqin Guo Lu Rui Nong Zhou 《Phyton-International Journal of Experimental Botany》 SCIE 2021年第5期1535-1547,共13页
Paris polyphylla var.yunnanensis is a traditional Chinese medicinal plant,in which polyphyllin as the main medicinal component is an important secondary metabolite with bioactivity.Arbuscular mycorrhizal fungi(AMF)hav... Paris polyphylla var.yunnanensis is a traditional Chinese medicinal plant,in which polyphyllin as the main medicinal component is an important secondary metabolite with bioactivity.Arbuscular mycorrhizal fungi(AMF)have multiple positive effects on plants,while it is not clear whether AMF increase the content of medicinal components in medicinal plants.In this study,a total of nine AMF treatments were laid to analyze the mycorrhizal effect on polyphyllin accumulation and PpHMGR and PpSE expression of P.polyphylla var.yunnanensis.AMF increased the content of polyphyllin in the cultivated variety with low relation to the increase of inoculation intensity.Polyphyllin I,II,and VII were identified and partly improved by AMF inoculation,dependent on AMF treatments and culture environments.Similarly,the PpHMGR and PpSE expression was induced by mycorrhization,dependent on AMF species,whilst the induction was more obvious in PpSE than in PpHMGR after mycorrhization.It concluded that the symbiotic relationship between P.polyphylla var.yunnanensis and AMF increased polyphyllin content level in the plant,which was associated with the up-regulation of PpSE transcripts. 展开更多
关键词 paris polyphylla var.yunnanensis Arbuscular mycorrhizal fungi POLYPHYLLIN 3-hydroxy-3-methylglutaryl CoA reductase(HMGR) squalene epoxidase(SE)
下载PDF
Determination of 10 Kinds of Inorganic Elements in Paris polyphylla Smith var. yunnanensis(Franch.) Hand.-Mazz.(and Polygerm Varieties) 被引量:1
5
作者 Yongxia KE Shaoshan ZHANG +3 位作者 Fang XIAO Pei QUN Zhe LIU Yuan LIU 《Medicinal Plant》 CAS 2018年第4期22-24,共3页
[Objectives] To determine the content of 10 kinds of inorganic elements(Pb,Cu,As,Cd,Hg,Zn,Fe,Mn,Sr and Cr) in samples of Paris polyphylla Smith var. yunnanensis( Franch.) Hand.-Mazz.( and polygerm varieties) produced ... [Objectives] To determine the content of 10 kinds of inorganic elements(Pb,Cu,As,Cd,Hg,Zn,Fe,Mn,Sr and Cr) in samples of Paris polyphylla Smith var. yunnanensis( Franch.) Hand.-Mazz.( and polygerm varieties) produced in different areas. [Methods] The wet digestion and technique of inductively coupled plasma optical emission spectrometry( ICP-OES) were used. [Results] Under the experimental conditions,elements were not related to each other,and several kinds of elements could be measured at the same time. The content of Pb exceeding the standard only in sample S4 of P. polyphylla Smith var. yunnanensis( and polygerm varieties),Hg was not detected in all samples,and the rest heavy metals did not exceed the limit. [Conclusions] This study was expected to provide a certain reference for the development and utilization of P. polyphylla Smith var. yunnanensis( and polygerm varieties). 展开更多
关键词 paris polyphylla Smith var. yunnanensis(Franch.) Hand.-Mazz. Inorganic elements Inductively coupled plasma Heavy metals
下载PDF
Mycorrhizal Fungal Effects on Growth,Antioxidant Capacity,and Medicine Quality of Paris polyphylla var.yunnanensis 被引量:1
6
作者 Nong Zhou Lingfeng Xu +3 位作者 Min Yang Dongqin Guo Qiuxia Gan Jingjing Zhao 《Phyton-International Journal of Experimental Botany》 SCIE 2021年第3期747-759,共13页
A field experiment was conducted to determine the effects of two commercial strains composed of mulple arbuscular mycorrhizal fungi(AMF)species on plant growth,antioxidant capacity,and medicine quality of Paris polyph... A field experiment was conducted to determine the effects of two commercial strains composed of mulple arbuscular mycorrhizal fungi(AMF)species on plant growth,antioxidant capacity,and medicine quality of Paris polyphylla var.yunnanensis in three subtropical soils from Wanzhou,Anshun and Baoshan in fields.The results showed that AMF inoculation enhanced the fungal colonization rate and activities of both succinate dehydrogenase and alkaline phosphatase,thereby,enhancing the mycorrhizal viability of P.polyphylla var.yunnanensis.The concentrations of photosynthetic pigments(chlorophyll a,b,a+b and carotenoids),soluble sugar,soluble protein and photosynthetic capacity were higher in AMF-inoculated plants than in non-AMF-treated plants in field.AMFtreated plants recorded higher activities of catalase,peroxidase and superoxide dismutase,and caused the reduction in malondialdehyde content,indicating lower oxidative damage,compared with non-AMF plants.Polyphyllin I,Polyphyllin II,Polyphyllin III,Polyphyllin IV and total polyphyllin contents were increased by AMF treatment.In conclusion,AMF improved the plant growth,antioxidant capacity and medicinal quality of P.polyphylla var.yunnanensis seedlings.Hereinto,AMF effects on the soil from Wanzhou was relatively greater than on other soils. 展开更多
关键词 paris polyphylla var.yunnanensis arbuscular mycorrhizal fungi growth and development medicine quality
下载PDF
Identification of Paris polyphylla Smith var. yunnanensis(Franch.) Hand.-Mazz.(Polygerm Varieties) Produced in Gaoligong Mountain Areas
7
作者 Zhe LIU Shaoshan ZHANG +3 位作者 Yongxia KE Fang XIAO Pei QUN Yuan LIU 《Medicinal Plant》 CAS 2018年第4期77-79,86,共4页
[Objectives] To make original plant and microscopic identification and study the traits of Paris polyphylla Smith var. yunnanensis( Franch.) Hand.-Mazz.( polygerm varieties) produced in Gaoligong Mountain areas,and pr... [Objectives] To make original plant and microscopic identification and study the traits of Paris polyphylla Smith var. yunnanensis( Franch.) Hand.-Mazz.( polygerm varieties) produced in Gaoligong Mountain areas,and provide experimental data for their identification and application. [Methods] The original plant powder was used,and paraffin sections and physicochemical properties were identified. [Results]P. polyphylla Smith var. yunnanensis( polygerm varieties) produced in Gaoligong Mountain areas showed obvious microscopic features and physicochemical properties. [Conclusions] This study was expected to provide certain reference for identification of original plant and medicine and formulation of quality standard for P. polyphylla Smith var. yunnanensis( polygerm varieties) produced in Gaoligong Mountain areas,and provided basis for their further research and development. 展开更多
关键词 Gaoligong Mountain areas paris polyphylla Smith var.yunnanensis(Franch.) Hand.-Mazz. Polygerm varieties IDENTIFICATION
下载PDF
Epigenetic Factors Altered in Dehisced Anther Correlated to Seed Dormancy in <i>Paris polyphylla</i>var. Yunnanensis
8
作者 Xia Cheng Bin Wang +4 位作者 Linyun Liu Zhen Zhao Xia Ling Feng Zhao Dingkang Wang 《Agricultural Sciences》 2019年第12期1517-1533,共17页
Paris polyphylla var. yunnanensis (Franch.), one of the best-known medicinal plants in China, has a dehiscent anther which physiologically work in pollination, however, the dehiscent anther always closes in response t... Paris polyphylla var. yunnanensis (Franch.), one of the best-known medicinal plants in China, has a dehiscent anther which physiologically work in pollination, however, the dehiscent anther always closes in response to darkness every day, and watering or raining every time. To explore this frequently closing and its unkown physiology, next-generation sequencing was performed, and the transcriptome was de novo assembled. RNA-sequencing was carried out in 15 samples including seven openning samples, four closed samples owing to darkness or watering, and tissue samples (leaf, petal, calyx, and stigma) were used for control. We obtained 72.75 GB data, assembled into 79,815 unigenes. Differentially expressed unigenes (DEGs) between opened and closed anther samples were 6231 and the DEGs between anther and control samples were 2831. Comparation between the two DEGs by KEGG enrichment showed that “plant hormone signal transduction” pathway is the most significant pathway for DEGs from closing anther vs. opening anther, and expression model of DEGs in the pathway might elicit change in germination and seed dormancy. Further examination of the action of the signal pathway on physiology showed “chromatin binding” function was prominent in “DNA binding” function of annotated DEGs between opened and closed anthers, of the 215 “chromatin binding” unigenes, 120 were involved in epigenetic silencing, and 50 of the epigenetic unigenes were directly related to germination or seed dormancy, strongly correlating anther closing to epigenetic modification and seed dormancy. These results were verified that at least three auxins involved in seed dormancy showed same expression patterns occurred in abnormal closing anther and seed embryo in Paris polyphylla var. yunnanensis. In conclusion, the information from transcriptome point out that frequent abnormal closing of dehiscent anthers possibly transfer the impact on seed dormancy, and epigenetic modification happened in closing may be the cause. 展开更多
关键词 Abnormal Closing Dehiscent ANTHER Epigenetic Modification paris polyphylla VAR. yunnanensis Seed DORMANCY
下载PDF
Investigation on the Resources of Paris Polyphylla var.yunnanensis
9
作者 Shicai WU Guo LI +5 位作者 Qiping WANG Wenli YANG Xuemei CHEN Jian FENG Ganghua LI Shaoshan ZHANG 《Medicinal Plant》 CAS 2020年第6期27-30,共4页
In this study,the resource distribution,morphological characteristics,artificial cultivation and market circulation of Paris Polyphylla var.yunnanensis and its polygerm varieties in Yunnan were investigated by referri... In this study,the resource distribution,morphological characteristics,artificial cultivation and market circulation of Paris Polyphylla var.yunnanensis and its polygerm varieties in Yunnan were investigated by referring to the research literature,field investigation and our planting experience.It is found that P.polyphylla var.yunnanensis is widely distributed in Yunnan Province,realizing large-scale artificial cultivation,while the wild resources of polygerm varieties are almost on the verge of extinction and are in urgent need of protection. 展开更多
关键词 paris polyphylla var.yunnanensis Resource distribution CULTIvatION INVESTIGATION
下载PDF
Parisaponin Ⅰ和Parisvietnaside A的NMR数据分析 被引量:5
10
作者 刘奕训 康利平 +6 位作者 赵玉 陈合兵 邹鹏 熊呈琦 赵阳 谭大维 马百平 《波谱学杂志》 CAS CSCD 北大核心 2009年第3期316-326,共11页
从滇重楼中分离得到2个甾体皂苷,利用1H NMR、13C NMR、1H-1H COSY、HSQC、HMBC、1D和2D TOCSY等多种核磁共振方法鉴定其结构分别为(25R)-26-O-β-D-吡喃葡萄糖基-3β,22α,26-三羟基-呋甾-5-烯-3-O-α-L-吡喃鼠李糖基-(1→2)-[α-L-呋... 从滇重楼中分离得到2个甾体皂苷,利用1H NMR、13C NMR、1H-1H COSY、HSQC、HMBC、1D和2D TOCSY等多种核磁共振方法鉴定其结构分别为(25R)-26-O-β-D-吡喃葡萄糖基-3β,22α,26-三羟基-呋甾-5-烯-3-O-α-L-吡喃鼠李糖基-(1→2)-[α-L-呋喃阿拉伯糖基-(1→4)]-β-D-吡喃葡萄糖苷(1,Parisaponin I)和(25R)-3β,5α,6β-三羟基-△7-螺甾烯-3-O-β-D-吡喃葡萄糖基-(1→3)-[α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷(2,paris-vietnaside A).对化合物1和2的1H NMR和13C NMR信号分别进行了归属和详细分析,并纠正了文献中的核磁数据归属错误.化合物2为首次从滇重楼中分离得到. 展开更多
关键词 核磁共振(NMR) 归属 2D NMR 甾体皂苷 滇重楼
下载PDF
Unraveling the serial glycosylation in the biosynthesis of steroidal saponins in the medicinal plant Paris polyphylla and their antifungal action 被引量:1
11
作者 Yuegui Chen Qin Yan +9 位作者 Yunheng Ji Xue Bai Desen Li Rongfang Mu Kai Guo Minjie Yang Yang Tao Jonathan Gershenzon Yan Liu Shenghong Li 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2023年第11期4638-4654,共17页
Sugar-sugar glycosyltransferases play important roles in constructing complex and bioactive saponins.Here,we characterized a series of UDP-glycosyltransferases responsible for biosynthesizing the branched sugar chain ... Sugar-sugar glycosyltransferases play important roles in constructing complex and bioactive saponins.Here,we characterized a series of UDP-glycosyltransferases responsible for biosynthesizing the branched sugar chain of bioactive steroidal saponins from a widely known medicinal plant Paris polyphylla var.yunnanensis.Among them,a 2'-O-rhamnosyltransferase and three 6'-O-glucosyltrasferases catalyzed a cascade of glycosylation to produce steroidal diglycosides and triglycosides,respectively.These UDP-glycosyltransferases showed astonishing substrate promiscuity,resulting in the generation of a panel of 24 terpenoid glycosides including 15 previously undescribed compounds.A mutant library containing 44 variants was constructed based on the identification of critical residues by molecular docking simulations and protein model alignments,and a mutant UGT91AH1^(Y187A)with increased catalytic efficiency was obtained.The steroidal saponins exhibited remarkable antifungal activity against four widespread strains of human pathogenic fungi attributed to ergosterol-dependent damage of fungal cell membranes,and 2'-O-rhamnosylation appeared to correlate with strong antifungal effects.The findings elucidated the biosynthetic machinery for their production of steroidal saponins and revealed their potential as new antifungal agents. 展开更多
关键词 paris polyphylla var.yunnanensis Steroidal saponins GLYCOSYLTRANSFERASES Sugar chain elongation Antifungal activity
原文传递
滇重楼根茎有效成分与土壤钾状况的关系研究 被引量:26
12
作者 王印 何忠俊 +4 位作者 段艳涛 梁社往 熊俊芬 曾睿 范茂攀 《西南农业学报》 CSCD 北大核心 2012年第3期950-953,共4页
通过对滇重楼(Paris polyphylla var.yunnanensis)主要分布区25个县(市)、75个采样点根茎的总皂甙、多糖含量与土壤全K和速效K含量的相关分析,探讨了土壤K素营养在滇重楼品质形成中的作用。结果显示,土壤全K和速效K含量与根茎中总皂甙... 通过对滇重楼(Paris polyphylla var.yunnanensis)主要分布区25个县(市)、75个采样点根茎的总皂甙、多糖含量与土壤全K和速效K含量的相关分析,探讨了土壤K素营养在滇重楼品质形成中的作用。结果显示,土壤全K和速效K含量与根茎中总皂甙含量呈极显著正相关,与根茎中多糖含量呈显著正相关。根茎总皂甙和多糖含量与根茎K含量呈显著正相关。研究表明,丰富的土壤全K和速效K供应有利于滇重楼根茎总皂甙和多糖的合成与积累。 展开更多
关键词 滇重楼 土壤K素 皂甙 多糖
下载PDF
滇重楼高效繁育和高产栽培研究 被引量:41
13
作者 李绍平 杨丽英 +5 位作者 杨斌 谷安宇 严世武 李林玉 王馨 和云 《西南农业学报》 CSCD 2008年第4期956-959,共4页
在丽江市鲁旬乡对云南稀缺名贵药材滇重楼进行种子与根茎繁殖、不同遮荫方武的栽培技术研究。结果显示:根茎切割繁殖与种子繁殖相比,由于种植周期短,根茎增重快,具有更高的经济效益,且带顶芽切块繁殖优于不带顶芽切块繁殖,但繁殖... 在丽江市鲁旬乡对云南稀缺名贵药材滇重楼进行种子与根茎繁殖、不同遮荫方武的栽培技术研究。结果显示:根茎切割繁殖与种子繁殖相比,由于种植周期短,根茎增重快,具有更高的经济效益,且带顶芽切块繁殖优于不带顶芽切块繁殖,但繁殖系数低且易感病害;滇重楼在遮荫网下种植效果较好,其透光率以20%~30%为宜。 展开更多
关键词 滇重楼 繁殖技术 栽培技术
下载PDF
云南重楼种子育苗技术研究 被引量:36
14
作者 陈翠 杨丽云 +2 位作者 吕丽芬 赵琪 袁理春 《中国中药杂志》 CAS CSCD 北大核心 2007年第19期1979-1983,共5页
目的:缩短云南重楼种子萌发和出苗所需时间,提高其出苗率;提出在生产中切实可行的云南重楼种子有性繁殖育苗方法。方法:考察不同温度、不同播种方式(播种时间、覆土厚度、地膜覆盖)等因素对云南重楼种子萌发和出苗的影响。结果:... 目的:缩短云南重楼种子萌发和出苗所需时间,提高其出苗率;提出在生产中切实可行的云南重楼种子有性繁殖育苗方法。方法:考察不同温度、不同播种方式(播种时间、覆土厚度、地膜覆盖)等因素对云南重楼种子萌发和出苗的影响。结果:云南重楼种子胚后熟和萌发的最适温度在18~20℃,在此温度下处理3~4个月以后,转至0~10℃低温处理2~4个月,可在翌年4月出苗;适宜的播种时间是4月份以前,育苗土覆盖厚度为1cm左右,播种后用黑色地膜进行覆盖,可提高出苗率。结论:上述试验结果可为云南重楼种子育苗提供操作参考。 展开更多
关键词 云南重楼 种子 发芽率 出苗率
下载PDF
云南重楼的甾体皂苷类成分 被引量:29
15
作者 刘海 张婷 +2 位作者 陈筱清 黄芸 王强 《中国天然药物》 SCIE CAS CSCD 2006年第4期264-267,共4页
目的:对重楼属(Paris)植物云南重楼(Paris polyphyllaSmith var.yunnanensis)的甾体皂苷类成分进行研究。方法:采用大孔树脂,硅胶柱色谱,Sephadex LH-20及RP-C18等技术分离纯化单体化合物,根据理化性质、波谱数据鉴定其结构。结果:从云... 目的:对重楼属(Paris)植物云南重楼(Paris polyphyllaSmith var.yunnanensis)的甾体皂苷类成分进行研究。方法:采用大孔树脂,硅胶柱色谱,Sephadex LH-20及RP-C18等技术分离纯化单体化合物,根据理化性质、波谱数据鉴定其结构。结果:从云南重楼乙醇提取物中分离并鉴定了9个甾体皂苷类化合物:(23S,25S)-3β,23,27-trihydroxyspirost-5-en-3-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside(1)、重楼皂苷Ⅰ(2)、重楼皂苷Ⅱ(3)、(25R)diosgenin-3-O-β-D-glucopyranoside(4),(25R)diosgenin-3-O-α-L-arabinofuranosyl(1→4)-β-D-glucopy-ranoside(5),(25R)diosgenin-3-O-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside(6),(25R)diosgenin-3-O-β-D-glu-copyranosyl(1→3)[α-L-rhamnopyranosyl(1→2)]-β-D-glucopyranoside(7),(25R)pennogenin-3-O-α-L-arabinofura-nosyl(1→4)[α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside(8),(25R)pennogenin-3-O-α-L-rhamnopyranosyl(1→4)-α-L-rhamnopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glucopyranoside(9)。结论:化合物1为新化合物。 展开更多
关键词 重楼属 云南重楼 甾体皂苷
下载PDF
超声波对滇重楼种子萌发能力的影响 被引量:20
16
作者 周浓 李自勤 +2 位作者 姜北 张采琼 杨绍群 《湖北农业科学》 北大核心 2012年第1期114-116,共3页
研究超声波处理时间、温度、功率对滇重楼(Paris polyphylla var.yunnanensis)种子萌发的影响,寻找其萌发的最佳条件。通过室内常规发芽试验,统计滇重楼种子在不同超声波处理时间、温度、功率条件下的发芽率和发芽指数。结果表明,滇重... 研究超声波处理时间、温度、功率对滇重楼(Paris polyphylla var.yunnanensis)种子萌发的影响,寻找其萌发的最佳条件。通过室内常规发芽试验,统计滇重楼种子在不同超声波处理时间、温度、功率条件下的发芽率和发芽指数。结果表明,滇重楼种子经适当的超声波处理,缩短了萌发时间,提高了发芽率和发芽指数。40 min、50℃、200 W的超声波处理条件最适宜于滇重楼种子萌发。 展开更多
关键词 滇重楼(paris polyphylla var.yunnanensis) 种子 超声波 萌发率 萌发指数
下载PDF
滇重楼的化学成分研究 被引量:32
17
作者 王羽 高文远 +3 位作者 袁理春 刘新桥 王书军 陈翠 《中草药》 CAS CSCD 北大核心 2007年第1期17-20,共4页
目的研究滇重楼Paris polyphylla var.yunnanensis根茎中的化学成分。方法利用正相硅胶柱色谱、葡聚糖凝胶Sephadex LH-20、反相制备色谱等手段进行分离纯化,并通过1H-NMR、13C-NMR、ESI-MS等波谱技术进行结构鉴定。结果从醋酸乙酯层中... 目的研究滇重楼Paris polyphylla var.yunnanensis根茎中的化学成分。方法利用正相硅胶柱色谱、葡聚糖凝胶Sephadex LH-20、反相制备色谱等手段进行分离纯化,并通过1H-NMR、13C-NMR、ESI-MS等波谱技术进行结构鉴定。结果从醋酸乙酯层中分离得到了7个化合物,分别鉴定为豆甾醇-3-O-β-D-葡萄糖苷(Ⅰ)、1-O-(β-D-葡萄糖基)-(2S,3S,4E,8E)-2-[(2′R)-2′-羟基十六酰氨基]-4(E),8(E)-十八二烯-1,3-二醇(Ⅱ)、β-蜕皮激素(Ⅲ)、薯蓣皂苷元-3-O-α-L-呋喃阿拉伯糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-葡萄糖苷(Ⅳ)、薯蓣皂苷元-3-O-α-L-吡喃鼠李糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-葡萄糖苷(Ⅴ)、薯蓣皂苷元-3-O-α-L-吡喃鼠李糖基(1→4)-α-L-吡喃鼠李糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-葡萄糖苷(Ⅵ)和偏诺皂苷元-3-O-α-L-呋喃阿拉伯糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-葡萄糖苷(Ⅶ)。结论化合物Ⅱ为首次从该属植物中分离得到。 展开更多
关键词 滇重楼 鞘脂类化合物 甾体皂苷
下载PDF
ICP-AES法测定滇重楼中的微量元素 被引量:32
18
作者 张金渝 王元忠 +1 位作者 金航 杨维泽 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2009年第8期2247-2249,共3页
探讨滇重楼中多种金属元素的测定方法,采用湿法硝酸-双氧水进行消解,用电感耦合等离子体原子发射光谱法(ICP-AES)同时测定试样中钾、镁及多种微量元素铅、铜、铁、铬、锌和锰等的方法。其检出限为0.0013~0.0846μg.mL-1,变异系数为0.7%... 探讨滇重楼中多种金属元素的测定方法,采用湿法硝酸-双氧水进行消解,用电感耦合等离子体原子发射光谱法(ICP-AES)同时测定试样中钾、镁及多种微量元素铅、铜、铁、铬、锌和锰等的方法。其检出限为0.0013~0.0846μg.mL-1,变异系数为0.7%~4.6%,回收率在93.7%~108.2%,结果令人满意。发现中药滇重楼中含有比较丰富的铁元素,微量元素在中药中可能起到一定的药理作用,用ICP-AES测定中药微量元素以评价中药价值具有重要的意义。 展开更多
关键词 ICP-AES 微量元素 滇重楼 硝酸-双氧水消解
下载PDF
云南重楼植物内生真菌的分离及抗菌活性筛选 被引量:26
19
作者 孙桂丽 陈有为 +6 位作者 夏国兴 吴少华 张琦 李治滢 李绍兰 杨丽源 黄荣 《微生物学杂志》 CAS CSCD 2005年第6期59-62,共4页
从云南重楼[Paris polyphylla Smithvar.yunnnanensis(Franch.)Hand.Mazz.]块状茎中分离出166株内生真菌,对其进行形态分类鉴定归于4目,6科,20个属,体现了云南重楼植物内生真菌的生物多样性特征。同时,选择与人类和植物相关的37株病原... 从云南重楼[Paris polyphylla Smithvar.yunnnanensis(Franch.)Hand.Mazz.]块状茎中分离出166株内生真菌,对其进行形态分类鉴定归于4目,6科,20个属,体现了云南重楼植物内生真菌的生物多样性特征。同时,选择与人类和植物相关的37株病原微生物作为抗菌活性筛选指示菌,进行了云南重楼植物内生真菌抗菌活性的初步研究。结果表明,4株内生真菌对细菌、植物致病真菌、皮肤致病真菌多种病原微生物具有显著抑制生长的作用。 展开更多
关键词 云南重楼 内生真菌 抗菌活性筛选
下载PDF
氮、磷、钾对滇重楼产量及皂苷含量的影响 被引量:20
20
作者 李金龙 熊俊芬 +4 位作者 张海涛 魏少飞 杨生超 何忠俊 张兴芬 《云南农业大学学报(自然科学版)》 CSCD 北大核心 2016年第5期895-901,共7页
滇重楼是名贵道地中药材,过度采挖已使其野生资源濒临灭绝,人工栽培是解决滇重楼资源危机的必然途径。合理施用氮、磷、钾肥可提高其药用部位产量和品质。本文通过"3414"田间小区肥料效应试验,设N:23 kg/667 m^2、P2O5:12 kg/... 滇重楼是名贵道地中药材,过度采挖已使其野生资源濒临灭绝,人工栽培是解决滇重楼资源危机的必然途径。合理施用氮、磷、钾肥可提高其药用部位产量和品质。本文通过"3414"田间小区肥料效应试验,设N:23 kg/667 m^2、P2O5:12 kg/667 m^2、K2O:30 kg/667 m^2为常规施肥水平,研究不同水平氮、磷、钾对滇重楼产量及皂苷含量的影响。结果表明:土壤氮、磷、钾的贡献率分别为77.97%,64.93%和136.52%,土壤供钾量丰富,供氮量中等,供磷量不足;增施氮肥和磷肥,不施钾肥可提高滇重楼的产量。由滇重楼根茎增产率的单因素、双因素和三因素肥料效应函数方程可知:单施氮、磷、钾肥对滇重楼根茎增产率影响较小,氮、磷、钾配施对滇重楼根茎增产率影响最大;三因素肥料效应方程推荐的最佳施肥量(N:34.5 kg/667 m^2,P2O5:18 kg/667 m^2,K2O:0 kg/667 m^2)所得滇重楼根茎增长率最高,为87%;适量的氮肥和磷肥可提高滇重楼新、老根茎的皂苷含量,增施钾肥会使滇重楼新根茎皂苷含量降低。当氮肥和磷肥施用量分别为23 kg/667 m^2和18 kg/667 m^2时,滇重楼新、老根茎总皂苷含量最高;钾肥施用量为0 kg/667 m^2时,滇重楼新根茎总皂苷含量最高。 展开更多
关键词 “3414”肥料试验 滇重楼 皂苷 产量
下载PDF
上一页 1 2 7 下一页 到第
使用帮助 返回顶部