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基于流式细胞术和K-mer分析的银缕梅属(Parrotia C.A.Mey.)植物基因组大小测定 被引量:7
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作者 张云燕 安宇 +5 位作者 林峰 马秋月 周翔宇 金亮 李朋富 王中生 《植物遗传资源学报》 CAS CSCD 北大核心 2021年第2期561-570,共10页
金缕梅科(Hamamelidaceae)银缕梅属(Parrotia C.A.Mey.)仅包含银缕梅(Parrotia subaequalis(H.T.Chang)R.M.Hao&H.T.Wei)和波斯铁木(Parrotia persica(DC.)C.A.Mey.)两种落叶阔叶乔木,其中银缕梅是我国华东地区特有的Ⅰ级濒危珍稀... 金缕梅科(Hamamelidaceae)银缕梅属(Parrotia C.A.Mey.)仅包含银缕梅(Parrotia subaequalis(H.T.Chang)R.M.Hao&H.T.Wei)和波斯铁木(Parrotia persica(DC.)C.A.Mey.)两种落叶阔叶乔木,其中银缕梅是我国华东地区特有的Ⅰ级濒危珍稀保护植物,属东亚第三纪孑遗成分;其姊妹种波斯铁木则间断分布于伊朗北部,属北极第三纪孑遗植物类群。本研究首次利用流式细胞术和K-mer分析方法对银缕梅属两姊妹种的基因组大小进行了测定,建立和优化了以萝卜(Raphanus sativus L.‘Saxa’)为内标、WPB(Woody plant buffer)为细胞核解离液的两种植物单倍体基因组的DNA含量(DNA C值)流式测定的适宜体系,旨在为金缕梅科银缕梅属植物的全基因组测序、基因组学研究、种质资源开发和利用以及物种保育等提供前期基础数据参考;同时也可为金缕梅科其他属、种的基因组大小测定提供借鉴。主要研究结果如下:(1)通过流式测定银缕梅基因组大小约为971.45±13.91 Mb,波斯铁木基因组大小约为890.52±24.69 Mb;(2)K-mer分析估测银缕梅基因组大小为951.70 Mb,杂合率为1.740%,重复序列比例为77.50%;波斯铁木基因组大小为858.50 Mb,杂合率为0.695%,重复序列占74.30%;(3)银缕梅属于高杂合和高重复基因组,波斯铁木则属于微杂合和高重复基因组。本研究的结果为银缕梅属植物后续基于DNA三代高通量测序技术的全基因组测序、组装及去冗余处理等工作提供了重要的数据参考。 展开更多
关键词 银缕梅 波斯铁木 基因组大小 流式细胞术 K-mer分析 基因组去冗余
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Primary Host Tree Species of the Gypsy Moth Lymantria dispar (Lepidoptera: Lymantriidae)in Hyrcanian Forests
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作者 G. Hajizadeh M. Reza-Kavosi 《Journal of Agricultural Science and Technology(B)》 2011年第3期342-346,共5页
Invasions by exotic insects and diseases are one of the most important threats to the stability and productivity of forest ecosystems around the world. Lymantria dispar (Lepidoptera: Lymantriidae) in Iran was obser... Invasions by exotic insects and diseases are one of the most important threats to the stability and productivity of forest ecosystems around the world. Lymantria dispar (Lepidoptera: Lymantriidae) in Iran was observed for the first time in 1937 by Jalal Afshar in oak forests of the Guilan state region. Since its introduction, gypsy moth has spread to the northern, western and south-western regions of Iran. The largest outbreaks of gypsy moth occurred in the Talesh Oak forests of the Hyrcanian zone in 1975. The gypsy moth is a highly polyphagous folivore species that feeds on over 300 species of woody plants. Among its most preferred hosts are oaks and aspens. Some trees are resistant to the gypsy moth including honey locust, black locust, silver maple, green ash, dogwood, sycamore, horse chestnut, firs, and tulip trees. Defoliation during severe outbreaks alters the appearance of forest habitats and leads to tree mortality and changes in stand composition. This study was conducted during 2008 and 2009 in an effort to determine the primary host tree species that the gypsy moth infests in the Hyrcanian forests of lran. For that purpose all egg masses in the area were counted and conclusively significant differences in defoliation levels among tree species were found. The primary host tree species of gypsy moth in Iran was Persian ironweed, Parrotia persica. 展开更多
关键词 Gypsy moth Lymantria dispar host tree species parrotia persica persian ironweed hyrcanian forests.
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