目的通过实施实验动物支气管鲍特杆菌、嗜肺巴斯德杆菌、多杀巴斯德杆菌和鼠棒状杆菌检测能力验证活动,达到评估实验动物检测机构的检验能力,提高实验动物质量检测水平的目的。方法2021年和2022年按照中国合格评定国家认可委员会(China ...目的通过实施实验动物支气管鲍特杆菌、嗜肺巴斯德杆菌、多杀巴斯德杆菌和鼠棒状杆菌检测能力验证活动,达到评估实验动物检测机构的检验能力,提高实验动物质量检测水平的目的。方法2021年和2022年按照中国合格评定国家认可委员会(China National Accreditation Service for Conformity Assessment,CNAS)相关准则设立NIFDC-PT-309和NIFDC-PT-363两个项目(分别检测实验动物样品中呼吸道病原菌支气管鲍特杆菌/嗜肺巴斯德杆菌、鼠棒状杆菌/多杀巴斯德杆菌),开展能力验证活动。制备4种实验动物呼吸道病原菌和阴性对照大肠埃希菌的冷冻干燥样品;均匀性和稳定性检验合格后,随机分组编号,将样品发放给参加单位,要求在规定时限提交检验报告和原始记录。反馈的结果与样品设置一致的判定为满意结果,不一致或未按时提交的判定为不满意结果。结果2021年共有29家实验室参加了NIFDC-PT-309项目,获得满意结果的实验室为27个,满意率为93.1%。2022年共有30家实验室参加了NIFDC-PT-363项目,满意率为100%。结论国内大多数实验动物质量检测机构具备较强的呼吸道病原菌检测能力,并且通过开展能力验证活动这一检测能力正在稳步提升。展开更多
In order to study the anti-bacterial infection effect of polysaccharide from Agaricus blazei Murrill on chickens, the experimental groups were orally administrated A. blazei polysaccharide at low dose and high dose, r...In order to study the anti-bacterial infection effect of polysaccharide from Agaricus blazei Murrill on chickens, the experimental groups were orally administrated A. blazei polysaccharide at low dose and high dose, respectively, for 14 d continuously, and then, the chickens in various groups were infected with Escherichia coli or Pasteurella pneumotropica , so as to observe the clinical symptoms of chickens and record the change in body weight. Anatomy was performed 14 d later, and the organ indices were determined, so as to study the anti-bacterial infection effect of A. blazei polysaccharide on chickens. The results showed that after bacterial infection, the high-dose A. blazei polysaccharide group was significantly differed from other groups in changes of body weight and organ indices. It indicates that oral administration of high concentration of A. blazei polysaccharide could promote the development of poultry organs, thereby improving the immunity of organisms.展开更多
文摘目的通过实施实验动物支气管鲍特杆菌、嗜肺巴斯德杆菌、多杀巴斯德杆菌和鼠棒状杆菌检测能力验证活动,达到评估实验动物检测机构的检验能力,提高实验动物质量检测水平的目的。方法2021年和2022年按照中国合格评定国家认可委员会(China National Accreditation Service for Conformity Assessment,CNAS)相关准则设立NIFDC-PT-309和NIFDC-PT-363两个项目(分别检测实验动物样品中呼吸道病原菌支气管鲍特杆菌/嗜肺巴斯德杆菌、鼠棒状杆菌/多杀巴斯德杆菌),开展能力验证活动。制备4种实验动物呼吸道病原菌和阴性对照大肠埃希菌的冷冻干燥样品;均匀性和稳定性检验合格后,随机分组编号,将样品发放给参加单位,要求在规定时限提交检验报告和原始记录。反馈的结果与样品设置一致的判定为满意结果,不一致或未按时提交的判定为不满意结果。结果2021年共有29家实验室参加了NIFDC-PT-309项目,获得满意结果的实验室为27个,满意率为93.1%。2022年共有30家实验室参加了NIFDC-PT-363项目,满意率为100%。结论国内大多数实验动物质量检测机构具备较强的呼吸道病原菌检测能力,并且通过开展能力验证活动这一检测能力正在稳步提升。
基金Supported by Tianjin Science and Technology Popularization Project(17KPHDSF00110)Education Reform,Innovation and Guidance Program for Teachers in Universities of TianJin Agricultural University(20171003)
文摘In order to study the anti-bacterial infection effect of polysaccharide from Agaricus blazei Murrill on chickens, the experimental groups were orally administrated A. blazei polysaccharide at low dose and high dose, respectively, for 14 d continuously, and then, the chickens in various groups were infected with Escherichia coli or Pasteurella pneumotropica , so as to observe the clinical symptoms of chickens and record the change in body weight. Anatomy was performed 14 d later, and the organ indices were determined, so as to study the anti-bacterial infection effect of A. blazei polysaccharide on chickens. The results showed that after bacterial infection, the high-dose A. blazei polysaccharide group was significantly differed from other groups in changes of body weight and organ indices. It indicates that oral administration of high concentration of A. blazei polysaccharide could promote the development of poultry organs, thereby improving the immunity of organisms.
