Cotton diseases represent a major challenge to cotton growth.Cloning of a cotton pathogen response gene and promoter is of great importance to improve disease resistance.In this study,a
Burkholderia cepacia （Bc） and Pseudomonas aeruginosa （Pa） are both biocontrol agents in agriculture and opportunistic human pathogens in hospitals. Effective management and utilization practice is needed to unders...Burkholderia cepacia （Bc） and Pseudomonas aeruginosa （Pa） are both biocontrol agents in agriculture and opportunistic human pathogens in hospitals. Effective management and utilization practice is needed to understand their characteristics and distribution in rice. During the last decade, the two opportunistic human pathogens were detected in 631 samples of rice seed and 117 samples of rice plant in plain, highland and mountainous rice growing areas of China. Bc and Pa were primarily differentiated by common bacteriological characteristics and pathogenic tests and then identified into species by Biolog and FAME tests. However, the genotypes of Bc still could not be distinguished. It has been noted that the Bc and Pa mainly existed in rice root with the highest distribution frequency in plain areas （ 6.1% and 16.1%） and lowest in the mountainous areas （1.0% and 7.8%）.展开更多
The highly pathogenic avian influenza （HPAI） H5N1 virus has caused several outbreaks in domestic poultry. Despite great efforts to control the spread of this virus, it continues to evolve and poses a substantial thr...The highly pathogenic avian influenza （HPAI） H5N1 virus has caused several outbreaks in domestic poultry. Despite great efforts to control the spread of this virus, it continues to evolve and poses a substantial threat to public health because of a high mortality rate. In this study, we sequenced whole genomes of eight H5N1 avian influenza viruses isolated from domestic poultry in eastern China and compared them with those of typical influenza virus strains. Phylogenetic analyses showed that all eight genomes belonged to clade 2.3.2.1 and clade 7.2, the two main circulating clades in China. Viruses that clustered in clade 2.3.2.1 shared a high degree of homology with H5N1 isolates located in eastern Asian. Isolates that clustered in clade 7.2 were found to circulate throughout China, with an east-to-west density gradient. Pathogenicity studies in mice showed that these isolates replicate in the lungs, and clade 2.3.2.1 viruses exhibit a notably higher degree of virulence compared to clade 7.2 viruses. Our results contribute to the elucidation of the biological characterization and pathogenicity of HPAI H5N1 viruses.展开更多
Gray mold causes considerable economic losses of fruit and vegetable production. The current study on Egyptian population structure of Botrytis cinerea demonstrates that this species is composed of four TE genotypes: ...Gray mold causes considerable economic losses of fruit and vegetable production. The current study on Egyptian population structure of Botrytis cinerea demonstrates that this species is composed of four TE genotypes: transposa, vacuma, boty and flipper types using transposable elements and sensitivity to the hydroxyanilide fungicide, fenhexamid. The results show that transposa is the predominant isolate type (63.6%) in the sampled populations of B. cinerea. However, the four isolate types are fenhexamid-sensitive regardless of location, host plant and plant organ. Additionally, B. cinerea isolates collected from different host plants do not exhibit any host preference using artificial infection test on lettuce. Furthermore, no relation is found between isolate type and aggressiveness and no divergence event has occurred among the isolates collected from different locations and host plants. The results suggest that host specialization of B. cinerea has not been occurred in the current sampled crops.展开更多
Objective:To isolate and characterize the bioactive secondary metabolites from Aspergillus ochraceus(A.ochraceus)MP2 fungi.Methods:The anti bacterial activity of marine sponge derived fungi A.ochraceus MP2 was thoroug...Objective:To isolate and characterize the bioactive secondary metabolites from Aspergillus ochraceus(A.ochraceus)MP2 fungi.Methods:The anti bacterial activity of marine sponge derived fungi A.ochraceus MP2 was thoroughly investigated against antagonistic human pathogens.The optimum inhibitory concentration of the fungi in the elite solvent was also determined.The promising extracts that showed good antimicrobial activity were subjected to further analytical separation to get individual distinct metabolites and the eluants were further identified by GC MS instrumental analysis.The molecular characterization of the elite fungal strains were done by isolating their genomic DNA and amplify the internal transcribed spacer(US)region of 5.8s rRNA using specific ITS primer.The novelty of the strain was proved by homology search tools and elite sequences was submitted to GENBANK.Results:Three bioactive compounds were characterized to reveal their identity,chemical formula and structure.The first elutant was identified asα-Campholene aldehyde with chemical fonnula C_(10)H_(16)O and molecular weight 152 Da.The second elutant was identified as Lucenin-2 and chemical fonnula C_(27)H_(30)O_(16)and molecular weight 610Da.The third elutant was identified as 6-Ethyloct-3-yl-2-ethylhexyl ester with Chemical fonnula C_(26)H_(42)O_4 with molecular weight 418 Da.Conclusions:The isolated compounds showed significant antimicrobial activity against potential human pathogens.Microbial secondary metabolites represent a large source of compounds endowed with ingenious structures and potent biological activities.展开更多
文摘Cotton diseases represent a major challenge to cotton growth.Cloning of a cotton pathogen response gene and promoter is of great importance to improve disease resistance.In this study,a
基金the National Natural Science Foundation of China （Nos. 30370951, 30270891）.
文摘Burkholderia cepacia （Bc） and Pseudomonas aeruginosa （Pa） are both biocontrol agents in agriculture and opportunistic human pathogens in hospitals. Effective management and utilization practice is needed to understand their characteristics and distribution in rice. During the last decade, the two opportunistic human pathogens were detected in 631 samples of rice seed and 117 samples of rice plant in plain, highland and mountainous rice growing areas of China. Bc and Pa were primarily differentiated by common bacteriological characteristics and pathogenic tests and then identified into species by Biolog and FAME tests. However, the genotypes of Bc still could not be distinguished. It has been noted that the Bc and Pa mainly existed in rice root with the highest distribution frequency in plain areas （ 6.1% and 16.1%） and lowest in the mountainous areas （1.0% and 7.8%）.
基金supported in part by the funding from the National Natural Scientific Foundation(81370518)the National High Technology Research and Development Program of China(2015AA020924 and 2013ZX10004003)supported by a grant from the Beijing Nova Program(No.Z141107001814054)
文摘The highly pathogenic avian influenza （HPAI） H5N1 virus has caused several outbreaks in domestic poultry. Despite great efforts to control the spread of this virus, it continues to evolve and poses a substantial threat to public health because of a high mortality rate. In this study, we sequenced whole genomes of eight H5N1 avian influenza viruses isolated from domestic poultry in eastern China and compared them with those of typical influenza virus strains. Phylogenetic analyses showed that all eight genomes belonged to clade 2.3.2.1 and clade 7.2, the two main circulating clades in China. Viruses that clustered in clade 2.3.2.1 shared a high degree of homology with H5N1 isolates located in eastern Asian. Isolates that clustered in clade 7.2 were found to circulate throughout China, with an east-to-west density gradient. Pathogenicity studies in mice showed that these isolates replicate in the lungs, and clade 2.3.2.1 viruses exhibit a notably higher degree of virulence compared to clade 7.2 viruses. Our results contribute to the elucidation of the biological characterization and pathogenicity of HPAI H5N1 viruses.
文摘Gray mold causes considerable economic losses of fruit and vegetable production. The current study on Egyptian population structure of Botrytis cinerea demonstrates that this species is composed of four TE genotypes: transposa, vacuma, boty and flipper types using transposable elements and sensitivity to the hydroxyanilide fungicide, fenhexamid. The results show that transposa is the predominant isolate type (63.6%) in the sampled populations of B. cinerea. However, the four isolate types are fenhexamid-sensitive regardless of location, host plant and plant organ. Additionally, B. cinerea isolates collected from different host plants do not exhibit any host preference using artificial infection test on lettuce. Furthermore, no relation is found between isolate type and aggressiveness and no divergence event has occurred among the isolates collected from different locations and host plants. The results suggest that host specialization of B. cinerea has not been occurred in the current sampled crops.
文摘Objective:To isolate and characterize the bioactive secondary metabolites from Aspergillus ochraceus(A.ochraceus)MP2 fungi.Methods:The anti bacterial activity of marine sponge derived fungi A.ochraceus MP2 was thoroughly investigated against antagonistic human pathogens.The optimum inhibitory concentration of the fungi in the elite solvent was also determined.The promising extracts that showed good antimicrobial activity were subjected to further analytical separation to get individual distinct metabolites and the eluants were further identified by GC MS instrumental analysis.The molecular characterization of the elite fungal strains were done by isolating their genomic DNA and amplify the internal transcribed spacer(US)region of 5.8s rRNA using specific ITS primer.The novelty of the strain was proved by homology search tools and elite sequences was submitted to GENBANK.Results:Three bioactive compounds were characterized to reveal their identity,chemical formula and structure.The first elutant was identified asα-Campholene aldehyde with chemical fonnula C_(10)H_(16)O and molecular weight 152 Da.The second elutant was identified as Lucenin-2 and chemical fonnula C_(27)H_(30)O_(16)and molecular weight 610Da.The third elutant was identified as 6-Ethyloct-3-yl-2-ethylhexyl ester with Chemical fonnula C_(26)H_(42)O_4 with molecular weight 418 Da.Conclusions:The isolated compounds showed significant antimicrobial activity against potential human pathogens.Microbial secondary metabolites represent a large source of compounds endowed with ingenious structures and potent biological activities.
