[Objectives] The study aimed to identify the pathogenic E. coli strain that caused diarrhea in foxes and to analyze its drug sensitivity.[Methods] A pathogenic E. coli strain was isolated from dead foxes with diarrhea...[Objectives] The study aimed to identify the pathogenic E. coli strain that caused diarrhea in foxes and to analyze its drug sensitivity.[Methods] A pathogenic E. coli strain was isolated from dead foxes with diarrhea. By conventional bacterial isolation and culture, morphological observation, pathogenicity test and K-B disc method, the isolated strain was identified as pathogenic E. coli .[Results] The isolated pathogen was highly sensitive to ceftriaxone, cefotaxime, ciprofloxacin and lincomycin, moderately sensitive to enrofloxacin, neomycin, gentamycin, spectinomycin, florfenicol, amikacin and polymyxin, and resistant to ampicillin, amoxicillin and doxycycline.[Conclusions] This study provided reference for the prevention and control of diarrheal diseases in foxes in Qinhuangdao region.展开更多
[Objective] The paper was to analyze the carrying status of high pathogenicity island （HPI） in pathogenic Escherichia coli of mink. [Method] Eight strains of E. coli were isolated from dead mink, and conducted patho...[Objective] The paper was to analyze the carrying status of high pathogenicity island （HPI） in pathogenic Escherichia coli of mink. [Method] Eight strains of E. coli were isolated from dead mink, and conducted pathogenicity test of artificial infection. The carrying status of HPI （irp2, fyuA） was detected by PCR. [Result] Eight strains of E. coli were pathogenic E. coli, and the carrying rate of HPI （irp2, fyuA） was 100%, positively correlated with the pathogenicity. [Conclusion] The results lay a foundation for further exploring the pathogenic mechanism of E. coli..展开更多
In order to understand the role of transmembrane signal transduction of host cells in the early steps of infection,the adherence of E. coli to HEp 2 cells and the change of activity of phospholipase C γ (PLC γ) indu...In order to understand the role of transmembrane signal transduction of host cells in the early steps of infection,the adherence of E. coli to HEp 2 cells and the change of activity of phospholipase C γ (PLC γ) induced by the adherence were investigated.The adherence of enteropathogenic E.coli (EPEC), strain E.7, induced a significant increase of inositol triphosphat (IP 3) level in HEp 2 cells. The adherence of the bacteria and the increase of IP 3 was kinetically correlated. Whereas the increase of IP 3 level induced by the adherence of the control strain EPEC (H511), a non piliated strain, was much meager than that by E7, a piliated strain. The results highlighted an important role of transmembrane signals like IP 3 in the pathogenesis of EPEC.展开更多
基金Supported by Project of Hebei Education Department(ZD2017234)Project of Science and Technology Bureau of Shijiazhuang(171500953A)Project of Science and Technology Bureau of Qinhuangdao(201602A046)
文摘[Objectives] The study aimed to identify the pathogenic E. coli strain that caused diarrhea in foxes and to analyze its drug sensitivity.[Methods] A pathogenic E. coli strain was isolated from dead foxes with diarrhea. By conventional bacterial isolation and culture, morphological observation, pathogenicity test and K-B disc method, the isolated strain was identified as pathogenic E. coli .[Results] The isolated pathogen was highly sensitive to ceftriaxone, cefotaxime, ciprofloxacin and lincomycin, moderately sensitive to enrofloxacin, neomycin, gentamycin, spectinomycin, florfenicol, amikacin and polymyxin, and resistant to ampicillin, amoxicillin and doxycycline.[Conclusions] This study provided reference for the prevention and control of diarrheal diseases in foxes in Qinhuangdao region.
基金Supported by Project of Hebei Department of Education(ZD2017234)Project of Shijiazhuang Science and Technology Bureau(171500953A)Project of Qinhuangdao Science and Technology Bureau(201602A046)
文摘[Objective] The paper was to analyze the carrying status of high pathogenicity island （HPI） in pathogenic Escherichia coli of mink. [Method] Eight strains of E. coli were isolated from dead mink, and conducted pathogenicity test of artificial infection. The carrying status of HPI （irp2, fyuA） was detected by PCR. [Result] Eight strains of E. coli were pathogenic E. coli, and the carrying rate of HPI （irp2, fyuA） was 100%, positively correlated with the pathogenicity. [Conclusion] The results lay a foundation for further exploring the pathogenic mechanism of E. coli..
基金This research was supported by the National Foundation ofNatural Sciences(NO.3947001)
文摘In order to understand the role of transmembrane signal transduction of host cells in the early steps of infection,the adherence of E. coli to HEp 2 cells and the change of activity of phospholipase C γ (PLC γ) induced by the adherence were investigated.The adherence of enteropathogenic E.coli (EPEC), strain E.7, induced a significant increase of inositol triphosphat (IP 3) level in HEp 2 cells. The adherence of the bacteria and the increase of IP 3 was kinetically correlated. Whereas the increase of IP 3 level induced by the adherence of the control strain EPEC (H511), a non piliated strain, was much meager than that by E7, a piliated strain. The results highlighted an important role of transmembrane signals like IP 3 in the pathogenesis of EPEC.
