Identification of C-geranylated flavonoids from Paulownia catalpifolia Gong Tong fruits by HPLC-DAD-ESI-MS/MS and their anti-aging effects on 2BS cells induced by H2O2

The fruits of Paulownia catalpifolia Gong Tong are used as a Chinese folk herbal medicine for the treatment of enteritis, tonsillitis, bronchitis, and dysentery, etc. Our previous study has identified new C-geranylated flavanones with obvious anti-proliferative effects in lung cancer A549 cells. In the present study, a new C-geranylated flavone, paucatalinone C(1) and five known C-geranylated flavanones(2-6) were isolated. In addition, a total of 34 C-geranylated flavonoids were detected by HPLC-DAD-ESI-MS/MS coupling techniques from the CH_2Cl_2 extract of P. catalpifolia. Futhermore, anti-aging effects of isolated compounds were evaluated in vitro with premature senescent 2BS cells induced by H_2O_2. Phytochemical results indicated that P. catalpifolia was a natural resource of abundant C-geranylated flavonoids. Diplacone(3) and paucatalinone A(5) were the potent anti-aging agents in the premature senescent 2BS cells induced by H_2O_2 and the C-geranyl substituent may be an important factor because of its lipophilic character.

楸叶泡桐果皮的化学成分研究

目的:研究楸叶泡桐果皮的化学成分。方法:采用硅胶、Sephadex LH-20、ODS柱色谱等方法对楸叶泡桐果皮的95%乙醇提取物进行分离纯化,根据化合物的波谱数据进行结构鉴定。结果:从楸叶泡桐果皮中分离纯化得到11个化合物,分别鉴定为:8-羟基-9-甲氧基-1,2,3,5,6,10b-六氢吡咯骈[2,1-a]异喹啉-3-酮(1)、8,9-二甲氧基-1,2,3,5,6,10b-六氢吡咯骈[2,1-a]异喹啉-3-酮(2)、2α,3α,19α-三羟基-12-烯-28-乌苏酸(3)、2α,3α,23-三羟基-12-烯-28-齐墩果酸(4)、myrianthic acid(5)、balanophonin(6)、tomentin B(7)、tomentin D(8)、tomentin E(9)、diplacone(10)、tomentin C(11)。结论:其中,化合物1、2为新的天然化合物,化合物6为首次从泡桐属中分离得到,化合物3~11为首次从该植物中分离得到。

楸叶泡桐种子的苯乙醇苷类化学成分研究

目的研究楸叶泡桐种子的化学成分。方法采用硅胶、Sephadex LH-20、ODS柱色谱等方法对楸叶泡桐种子的70%乙醇提取物进行分离纯化,根据化合物的波谱数据进行结构鉴定。结果从楸叶泡桐种子中分离纯化得到7个苯乙醇苷类化合物,分别鉴定为:ilicifolioside A和isoilicifolioside A混合物(1),isoacteoside(2),plantalloside(3),orobanchoside(4),darendoside B(5),acteoside(6),tomentoside(7)。结论其中,化合物3,4,5为首次从泡桐属中分离得到,化合物1,2,6,7为首次从该植物中分离得到。