The effects of oxytetracycline on the morphology of the seedling with witches’ broom and global DNA methylation level of Paulownia tomentosa × Paulownia fortunei were investigated.The results indicated that the ...The effects of oxytetracycline on the morphology of the seedling with witches’ broom and global DNA methylation level of Paulownia tomentosa × Paulownia fortunei were investigated.The results indicated that the infected seedlings by phytoplasma recovered to normal morphology of the healthy seedlings after being treated with more than 20 mg L-1 oxytetracycline,and the pathogen could not be detected with PCR in the seedlings.The global DNA methylation level of diseased seedlings was lower than that of healthy one.The oxytetracycline treatment was able to increase the DNA methylation level,and the methylation level of the diseased seedlings increased with the dose of applied oxytetracycline.However the increased methylation of infected seedlings with oxytetracycline treatment could still not reach to level of healthy seedling（CK）,even the seedlings treated with lethal oxytetracycline concentration.展开更多
Inorder to carry out trunk extension by lateral buds ,the laws of trunk extension and branching sprouted from lateral buds were investigated in 1-year-old seedlings of P.tomentosaxP,fortunei 33 under natural condition...Inorder to carry out trunk extension by lateral buds ,the laws of trunk extension and branching sprouted from lateral buds were investigated in 1-year-old seedlings of P.tomentosaxP,fortunei 33 under natural conditions.The results indicated：（1）Under conditions of high density and extensive management,42%of individuals in P.tomentosaxP.fortunei 33 showed pseudo dichotomous branching characteristics and could not extend their trunks in the second year.But other individuals showed the characteristics of sympodial branching and pseudo monopodial branching （not from terminal buds ）and could realize natural trunk extension.All the 3types of branching could be called branching of pseudo asis;（2）The stem form quotieties of extended trunk in all individuals wrer larger in the second year ,but only 14%of them could straight extend their trunks and 86%of them needed2-3years to reach straight trunk estension;（3）Among individuals which could extend their trunks ,from upper lateral buds to lower lateral buds on trunks ,the lengths and diameters of branches sprouted from lateral buds became smaller and smaller ,but the angles of branching up to trunk became larger and larger ,and the fourth pair of lateral branches reached to the largest,Under the fourth pair ,the angles of branches became smaller again and those branches all died.展开更多
文摘The effects of oxytetracycline on the morphology of the seedling with witches’ broom and global DNA methylation level of Paulownia tomentosa × Paulownia fortunei were investigated.The results indicated that the infected seedlings by phytoplasma recovered to normal morphology of the healthy seedlings after being treated with more than 20 mg L-1 oxytetracycline,and the pathogen could not be detected with PCR in the seedlings.The global DNA methylation level of diseased seedlings was lower than that of healthy one.The oxytetracycline treatment was able to increase the DNA methylation level,and the methylation level of the diseased seedlings increased with the dose of applied oxytetracycline.However the increased methylation of infected seedlings with oxytetracycline treatment could still not reach to level of healthy seedling（CK）,even the seedlings treated with lethal oxytetracycline concentration.
基金Foundation of Research Startup for Returned Student From Abroad Science Foundation of Henan Province forDistinguished Young Scholars(No.002).
文摘Inorder to carry out trunk extension by lateral buds ,the laws of trunk extension and branching sprouted from lateral buds were investigated in 1-year-old seedlings of P.tomentosaxP,fortunei 33 under natural conditions.The results indicated：（1）Under conditions of high density and extensive management,42%of individuals in P.tomentosaxP.fortunei 33 showed pseudo dichotomous branching characteristics and could not extend their trunks in the second year.But other individuals showed the characteristics of sympodial branching and pseudo monopodial branching （not from terminal buds ）and could realize natural trunk extension.All the 3types of branching could be called branching of pseudo asis;（2）The stem form quotieties of extended trunk in all individuals wrer larger in the second year ,but only 14%of them could straight extend their trunks and 86%of them needed2-3years to reach straight trunk estension;（3）Among individuals which could extend their trunks ,from upper lateral buds to lower lateral buds on trunks ,the lengths and diameters of branches sprouted from lateral buds became smaller and smaller ,but the angles of branching up to trunk became larger and larger ,and the fourth pair of lateral branches reached to the largest,Under the fourth pair ,the angles of branches became smaller again and those branches all died.
